Transcriptome sequencing reveals the effect of selenium nanoparticles on primary hepatocytes of rainbow trout

Heat stress exposure cause alterations in intestinal microbiota, transcriptome, and metabolome of broilers

“…The FoxO signaling pathway is involved in various cellular functions, including cell proliferation, apoptosis, autophagy, oxidative stress, and metabolic disorders ( Xing et al, 2018 ). According to reports, under environmental stress conditions, the FoxO signaling pathway in fish is significantly enriched, which is consistent with our research results ( Shang et al, 2023 ; Sun J. et al, 2023 ). The ROS produced by heat stress can regulate the expression of FoxO at the levels of transcription, protein activation, phosphorylation, and acetylation and overactivation and overexpression of FoxO can lead to the occurrence of various diseases ( Liu et al, 2023 ).…”

A review of the quantitative real-time PCR and Omics approaches applied to study the effects of dietary selenium nanoparticles (nano-Se) on fish

“… [98] 19 RNA-seq Rainbow trout ( Oncorhynchus mykiss ) Hepatocytes Analyzing the transcriptome profile of nano-Se spiked hepatocytes after heat stress Differentially expressed genes belong to five immune-related pathways including FoxO signaling, RIG-I-like receptor signaling, C-type lectin receptor signaling, Toll-like receptor signaling, and Rachidonic acid metabolism. [96] 20 qRT-PCR Onychostoma macrolepis Liver Evaluating the antibacterial effects of the nano-Se extracted from Bacillus subtilis and its efficacy in inhibition of ferroptosis Up-regulation of the GPx4, Nrf2, and FPN1 genes and inhibition of ferroptosis in fish liver [100] Proteomics 21 2DE, MALDI-TOF/TOF MS Rainbow trout ( Oncorhynchus mykiss ) Liver Analysis of nano-Se effects on liver proteome Up-regulation of genes involved in aerobic glycolytic pathways, amino acid metabolism, and energy-producing pathways, up-regulation of two anti-tumor proteins [124] 22 DIA, SWATH Rainbow trout ( Oncorhynchus mykiss ) Liver Effects of nano-Se as an anti-heat stress micronutrient on liver proteome Inhibition of apoptosis by up-regulation of the HSP family and selenoproteins and down-regulation of proteins related to lipid metabolism [125] Metabolomics/ Metabonomics 23 LC-MS Rainbow trout ( Oncorhynchus mykiss ) Liver The protective effect of nano-Se against heat stress Nano-Se alleviates heat stress via the down-regulation of galactose metabolism and activation of pathways responsible for glutamate and glutamine metabolism [129] Muti-omics 24 Proteomics: LC/Orbitrap MS Transcriptomics: RNA-seq Zebrafish ( Danio rerio ) Proteomics: serum Transcriptomics: liver and kidney Identification of molecular mechanisms underlying immunomodulation of nano-Se in diseased fish Nano-Se exerts beneficial effects via the regulation of genes involved in antioxidant defense, lipid metabolism, and innate immunity [132]...…”

“…This conclusion was supported by the higher expression rates of apoptosis-related genes (caspase3, caspase9, Cyt-c, Bax, and Bax/Bcl-2) and lower mRNA levels of P13K and AKT genes in the liver of heat-stressed fish without nano-Se supplementation. These researchers also added 5 μg/mL of nano-Se to cultured hepatocytes of rainbow trout and studied the response of hepatocytes to heat stress using transcriptome sequencing [96] . Transcriptome profiling of the nano-Se-treated hepatocytes demonstrated that most of the differentially expressed genes (DEGs) were related to immune function and belong to five immune-related pathways, including FoxO signaling, RIG-I-like receptor signaling, C-type lectin receptor signaling, Toll-like receptor signaling, and Rachidonic acid metabolism.…”