Transcriptome sequencing of <i>Verticillium dahliae</i> from a cotton farm reveals positive correlation between virulence and tolerance of sugar-induced hyperosmosis

Li, Jin; Perucho, Juan; Liu, Yuanyuan; Xia, Wenwen; Cheng, Fengfeng; Tian, Wenhui; Zhang, Lin; Zhu, Jianbo; Wang, Aiying

doi:10.7717/peerj.8035

Cited by 2 publications

(2 citation statements)

References 69 publications

(72 reference statements)

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“…Regarding the interaction between V. dahliae and cotton, previous studies have mostly focused on either the pathogen or the host at the early infection stage 20 . In the present study, we found two distinct stages during the molecular interaction through time-course transcriptome analysis.…”

Section: Discussionmentioning

confidence: 99%

Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis

Qiu,

Li,

Zhang

et al. 2023

Nat Commun

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Verticillium dahliae is a soil-borne hemibiotrophic fungal pathogen that threatens cotton production worldwide. In this study, we assemble the genomes of two V. dahliae isolates: the more virulence and defoliating isolate V991 and nondefoliating isolate 1cd3-2. Transcriptome and comparative genomics analyses show that genes associated with pathogen virulence are mostly induced at the late stage of infection (Stage II), accompanied by a burst of reactive oxygen species (ROS), with upregulation of more genes involved in defense response in cotton. We identify the V991-specific virulence gene SP3 that is highly expressed during the infection Stage II. V. dahliae SP3 knock-out strain shows attenuated virulence and triggers less ROS production in cotton plants. To control the disease, we employ polyethyleneimine-coated MXene quantum dots (PEI-MQDs) that possess the ability to remove ROS. Cotton seedlings treated with PEI-MQDs are capable of maintaining ROS homeostasis with enhanced peroxidase, catalase, and glutathione peroxidase activities and exhibit improved tolerance to V. dahliae. These results suggest that V. dahliae trigger ROS production to promote infection and scavenging ROS is an effective way to manage this disease. This study reveals a virulence mechanism of V. dahliae and provides a means for V. dahliae resistance that benefits cotton production.

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Section: Discussionmentioning

confidence: 99%

Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis

Qiu,

Li,

Zhang

et al. 2023

Nat Commun

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show abstract

“…Regarding the interaction between V. dahliae and cotton, previous studies have mostly focused on either the pathogen or the host at the early infection stage 20 . Here, we found two distinct stages in molecular interaction through a longer-term transcriptome analysis.…”

Section: Discussionmentioning

confidence: 99%

Polyethyleneimine coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis

Qiu

Zhang

et al. 2022

Preprint

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Verticillium dahliae is a soil-borne hemi-biotrophic fungal pathogen and inflicts major losses in agricultural production worldwide. Although the pathogenicity of the fungus and plant immunity have been extensively studied, how cotton leaf necrosis is induced by defoliating strains of V. dahliae is poorly understood. In this study, reference-grade genomes were determined for two representative V. dahliae isolates: V991, which is defoliating and aggressive, and 1cd3-2, which is non-defoliating and has weak pathogenicity. Transcriptome analysis showed that cotton resistance to V. dahliae mainly depends on cell wall structure or physical barrier generation in the early stage of infection (3-9 days post inoculation, dpi). However, a large number of pathogenic factors could be detected from 12 dpi and accumulated quickly in cotton stems, accompanied by the burst of reactive oxygen species (ROS), leaf necrosis and defoliation. A V991 specific virulence gene SP3 was identified through comparative genome analysis and found to be highly expressed after colonization. Knock-out of SP3 clearly attenuated pathogenicity, with less ROS produced. These results indicate that disease symptoms in cotton may be due to abnormal immune activation and excessive ROS induced by the pathogen. To further investigate this, Polyethyleneimine coated MXene quantum dots (PEI-MQDs), a type of nano material that possesses the ability to remove ROS in vitro, were used. Cotton seedlings maintain ROS homeostasis with enhanced peroxidase (POD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities after exogenous treatment with PEI-MQDs and show significant tolerance to V. dahliae isolate V991. Our results suggest that excessive ROS and constitutive immune activation in cotton induced by the defoliating strain of V. dahliae is responsible for disease symptoms such as leaf necrosis, and PEI-MQDs application improve cotton tolerance to V. dahliae by maintaining ROS homeostasis.

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Transcriptome sequencing of Verticillium dahliae from a cotton farm reveals positive correlation between virulence and tolerance of sugar-induced hyperosmosis

Cited by 2 publications

References 69 publications

Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis

Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis

Polyethyleneimine coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis

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