Transcriptome Sequencing Data of Bacillus anthracis Vollum Δ
            <i>htrA</i>
            and Its Parental Strain, Isolated under Oxidative Stress

Chitlaru, Theodor; Cohen-Gihon, Inbar; Israeli, Ofir; Elia, Uri; Zaide, Galia; Israeli, Moshe; Beth-Din, Adi; Lazar, Shirley; Ehrlich, Sharon; Zvi, Anat; Cohen, Ofer

doi:10.1128/mra.00618-20

Cited by 2 publications

(4 citation statements)

References 10 publications

(11 reference statements)

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“…The resulting phylogenetic tree, depicted in the Supplementary Fig. 1 [23] was shaped with the iTOL: interactive tree of life platform [24]. This analysis established that the novel MWH2021 strain is adjacent in the phylogenetic clustering to strain MAA2018 [10,11], in line with their common geographic origin (India).…”

Section: Data Descriptionmentioning

confidence: 81%

“…Bioinformatic DNA similarity analysis of the genomic sequences of the two novel clinical isolates, for determining their phylogenetic relation to other B. pseudomallei strains [23,28], was conducted using 131 complete B. pseudomallei genomes present in the NCBI databank (as of June 2023). It is conceivable that conducting the analysis by comparison to all available B. pseudomallei genomes (not only complete ones), may have provided a more comprehensive phylogenetic profile.…”

Section: Limitationmentioning

confidence: 99%

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Genome sequence of two novel virulent clinical strains of Burkholderia pseudomallei isolated from acute melioidosis cases imported to Israel from India and Thailand

Cohen-Gihon,

Zaide,

Amit

et al. 2024

BMC Genom Data

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Objective Burkholderia pseudomallei, the etiological cause of melioidosis, is a soil saprophyte endemic in South-East Asia, where it constitutes a public health concern of high-priority. Melioidosis cases are sporadically identified in nonendemic areas, usually associated with travelers or import of goods from endemic regions. Due to extensive intercontinental traveling and the anticipated climate change-associated alterations of the soil bacterial flora, there is an increasing concern for inadvertent establishment of novel endemic areas, which may expand the global burden of melioidosis. Rapid diagnosis, isolation and characterization of B. pseudomallei isolates is therefore of utmost importance particularly in non-endemic locations. Data description We report the genome sequences of two novel clinical isolates (MWH2021 and MST2022) of B. pseudomallei identified in distinct acute cases of melioidosis diagnosed in two individuals arriving to Israel from India and Thailand, respectively. The data includes preliminary genetic analysis of the genomes determining their phylogenetic classification in rapport to the genomes of 131 B. pseudomallei strains documented in the NCBI database. Inspection of the genomic data revealed the presence or absence of loci encoding for several documented virulence determinants involved in the molecular pathogenesis of melioidosis. Virulence analysis in murine models of acute or chronic melioidosis established that both strains belong to the highly virulent class of B. pseudomalleii.

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Section: Data Descriptionmentioning

confidence: 81%

Section: Limitationmentioning

confidence: 99%

Genome sequence of two novel virulent clinical strains of Burkholderia pseudomallei isolated from acute melioidosis cases imported to Israel from India and Thailand

Cohen-Gihon,

Zaide,

Amit

et al. 2024

BMC Genom Data

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“…The expression data of each particular gene from duplicated or triplicated experimental groups were averaged prior to the analysis. The transcriptomic data have been deposited to the NCBI database [55]. The transcriptomes determined in the absence of H 2 O 2 (i.e., samples 1 and 3 for the WT strain, and 2 and 5 for the ∆htrA strain, see Figure 2A) were highly similar.…”

Section: Overview Of the Transcriptome Analysis In Response To Oxidat...mentioning

confidence: 98%

“…Default settings in all analytical software programs were used. The transcriptomic data have been deposited to the NCBI database [55]. GEO (Gene Expression Omnibus) accession of the entire transcriptome series: GSE151208.…”

Section: Rna Sequencing Transcriptome Assembly and Differential Expre...mentioning

confidence: 99%

Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis htrA-Disrupted and Parental Wild Type Strains

et al. 2020

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We previously demonstrated that the HtrA (High Temperature Requirement A) protease/chaperone active in the quality control of protein synthesis, represents an important virulence determinant of Bacillus anthracis. Virulence attenuation of htrA-disrupted Bacillus anthracis strains was attributed to susceptibility of ΔhtrA strains to stress insults, as evidenced by affected growth under various stress conditions. Here, we report a comparative RNA-seq transcriptomic study generating a database of differentially expressed genes in the B. anthracishtrA-disrupted and wild type parental strains under oxidative stress. The study demonstrates that, apart from protease and chaperone activities, HtrA exerts a regulatory role influencing expression of more than 1000 genes under stress. Functional analysis of groups or individual genes exhibiting strain-specific modulation, evidenced (i) massive downregulation in the ΔhtrA and upregulation in the WT strains of various transcriptional regulators, (ii) downregulation of translation processes in the WT strain, and (iii) downregulation of metal ion binding functions and upregulation of sporulation-associated functions in the ΔhtrA strain. These modulated functions are extensively discussed. Fifteen genes uniquely upregulated in the wild type strain were further interrogated for their modulation in response to other stress regimens. Overexpression of one of these genes, encoding for MazG (a nucleoside triphosphate pyrophosphohydrolase involved in various stress responses in other bacteria), in the ΔhtrA strain resulted in partial alleviation of the H2O2-sensitive phenotype.

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Transcriptome Sequencing Data of Bacillus anthracis Vollum Δ htrA and Its Parental Strain, Isolated under Oxidative Stress

Cited by 2 publications

References 10 publications

Genome sequence of two novel virulent clinical strains of Burkholderia pseudomallei isolated from acute melioidosis cases imported to Israel from India and Thailand

Genome sequence of two novel virulent clinical strains of Burkholderia pseudomallei isolated from acute melioidosis cases imported to Israel from India and Thailand

Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress of Bacillus anthracis htrA-Disrupted and Parental Wild Type Strains

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