Transcriptome Sequencing and De Novo Analysis of a Cytoplasmic Male Sterile Line and Its Near-Isogenic Restorer Line in Chili Pepper (Capsicum annuum L.)

Liu, Chen; Ma, Ning; Wang, Ping-Yong; Fu, Nan; Shen, Huolin

doi:10.1371/journal.pone.0065209

Cited by 66 publications

(55 citation statements)

References 70 publications

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“…The only source of CMS in this species is S cytoplasm from the ‘PI 164835’ line introduced from India (Peterson , Liu et al. ). Kim and Kim () developed two CMS‐specific SCAR markers to distinguish N cytoplasm from S cytoplasm by PCR.…”

Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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Section: Pollination Control Methods In Different Vegetable Cropsmentioning

confidence: 99%

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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“…Furthermore, partial fertility restoration has been reported (Lee, 2001;Lee and Park, 2008) with multiple haplotypes in existence (Min et al, 2008). Liu et al (2013) found differential expression of several PPR genes between near-isogenic lines for Rf and CMS in chile pepper. Although most Rf genes have been found to encode PPR proteins, some non-PPR genes have been identified to be closely associated with restoration-of-fertility in chile pepper.…”

mentioning

confidence: 90%

Genome-wide Identification of Chile Pepper Pentatricopeptide Repeat Domains Provides Insight into Fertility Restoration

Barchenger¹,

Said²,

Zhang³

et al. 2018

J. Amer. Soc. Hort. Sci.

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ADDITIONAL INDEX WORDS. bioinformatics, chile pepper, cytoplasmic male sterility, hybrid breeding, PPR, restorer of fertility, single nucleotide polymorphism ABSTRACT. Pentatricopeptide repeat (PPR) proteins constitute one of the largest protein families in plants. They are typically targeted to the mitochondria or chloroplast and bind to one or more organellar transcripts, influencing expression. Genes responsible for inhibiting the mitochondrial genes that cause cytoplasmic male sterility (CMS), restorer-of-fertility (Rf) genes, often encode PPRs. Using in silico techniques, 552 PPR domains were identified throughout the chile pepper (Capsicum annuum) genome. The domains were mapped across 12 chromosomes and were found to be largely distally or proximally located. About 28% of the chile pepper PPR domains identified in this study have high structural similarity to previously reported PPRs in arabidopsis (Arabidopsis thaliana). In addition, 11 candidate Rf genes clustered on chromosome 6, and 1 on chromosome 1 were identified that were characterized in 16 A-(S rfrf), B-(N rfrf), and C-line (N RfRf) backgrounds. These findings support a multigene model for fertility restoration and broaden our understanding of the restoration of fertility. This may be an explanation for the lack of widely applicable molecular markers for this important trait. With this new information, specific Rf markers may be developed and will facilitate the implementation of hybrid breeding programs in chile pepper. In addition, this work provides a basis for future research in PPRs, an increasingly important gene family.

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“…Recently, the transcriptome profiles of sterile lines and their fertility restorers have been investigated in various species during specific developmental stages, thereby demonstrating that a large number of differentially expressed genes (DEGs) participate in many metabolic pathways, e.g., in Brassica napus (An et al, 2014), cotton (Suzuki et al, 2013), cabbage (Wang et al, 2016), chili pepper ( Capsicum annuum ) (Liu et al, 2013), kenaf (Chen et al, 2014), watermelon (Rhee et al, 2015), radish (Mei et al, 2016), rice (Bai et al, 2015), and Welsh onion (Liu et al, 2016). However, the complexity of the wheat genome and the lack of complete reference genome mean that transcriptome sequencing in male sterile wheat remains difficult.…”

Section: Introductionmentioning

confidence: 99%

Identification of Candidate Genes and Biosynthesis Pathways Related to Fertility Conversion by Wheat KTM3315A Transcriptome Profiling

Duan

Gan

et al. 2017

Front. Plant Sci.

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The Aegilops kotschyi thermo-sensitive cytoplasmic male sterility (K-TCMS) system may facilitate hybrid wheat (Triticum aestivum L.) seed multiplication and production. The K-TCMS line is completely male sterile during the normal wheat-growing season, whereas its fertility can be restored in a high-temperature environment. To elucidate the molecular mechanisms responsible for male sterility/fertility conversion and candidate genes involved with pollen development in K-TCMS, we employed RNA-seq to sequence the transcriptomes of anthers from K-TCMS line KTM3315A during development under sterile and fertile conditions. We identified 16840 differentially expressed genes (DEGs) in different stages including15157 known genes (15135 nuclear genes and 22 plasmagenes) and 1683 novel genes. Bioinformatics analysis identified possible metabolic pathways involved with fertility based on KEGG pathway enrichment of the DEGs expressed in fertile and sterile plants. We found that most of the genes encoding key enzyme in the phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were significant upregulated in uninucleate, binuclate or trinucleate stage, which both interact with MYB transcription factors, and that link between all play essential roles in fertility conversion. The relevant DEGs were verified by quantitative RT-PCR. Thus, we suggested that phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were involved in fertility conversion of K-TCMS wheat. This will provide a new perspective and an effective foundation for the research of molecular mechanisms of fertility conversion of CMS wheat. Fertility conversion mechanism in thermo-sensitive cytoplasmic male sterile/fertile wheat involves the phenylpropanoid biosynthesis pathway, jasmonate biosynthesis pathway, and MYB transcription factors.

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Transcriptome Sequencing and De Novo Analysis of a Cytoplasmic Male Sterile Line and Its Near-Isogenic Restorer Line in Chili Pepper (Capsicum annuum L.)

Cited by 66 publications

References 70 publications

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Genome-wide Identification of Chile Pepper Pentatricopeptide Repeat Domains Provides Insight into Fertility Restoration

Identification of Candidate Genes and Biosynthesis Pathways Related to Fertility Conversion by Wheat KTM3315A Transcriptome Profiling

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