Transcriptome sequencing and comparative analysis of the gametophyte thalli of Pyropia tenera under normal and high temperature conditions

Choi, San; Hwang, Mi Sook; Im, Sungoh; Kim, Namju; Jeong, Won-Joong; Park, Eunjeong; Gong, Yong-Gun; Choi, Dong-Woog

doi:10.1007/s10811-012-9921-2

Cited by 38 publications

(27 citation statements)

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“…The transcriptome sequences from P. tenera gametophyte thalli have been generated under normal growth and heat stress conditions (Choi et al 2013), and attempts were made to identify the differentially expressed genes (DEG) under heat stress conditions. Among the identified DEGs, the most popular and well-known genes were small heat shock proteins (sHSPs), which constitute a small and highly conserved family in eukaryotes and prokaryotes and are highly involved in the heat shock response (Choi et al 2013;Waters, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Among the identified DEGs, the most popular and well-known genes were small heat shock proteins (sHSPs), which constitute a small and highly conserved family in eukaryotes and prokaryotes and are highly involved in the heat shock response (Choi et al 2013;Waters, 2013). The transcriptome sequences were screened for sHSP members, and 19 contigs encoding putative alpha crystalline domain (α-crystalline, ACD) were obtained.…”

Section: Resultsmentioning

confidence: 99%

“…Transcriptome sequence reads generated from P. tenera gametophyte thalli were analyzed for the sHSP gene family (Choi et al 2013). The contigs encoding putative sHSPs were analyzed to determine if they covered the full open reading frame (ORF).…”

Section: Ptshsp 193 Cdna Isolation and Sequence Analysismentioning

confidence: 99%

“…Pyropia grow naturally in the intertidal zone, where they are frequently exposed to a variety of potentially stressful environmental including variations in humidity, changes in temperature, osmotic shock and different light intensities (Hwang et al 1997;Blouin et al 2011). Therefore, Pyopia have developed a variety of strategies and mechanisms to respond to and survive through those environmental stresses (Choi et al 2013;Im et al 2015). Pyropia exhibits a exhibits a heteromorphic life cycle with an alternation of generation between foliose thallus gametophyte and filamentous sporophyte; this is referred to as conchocelis phase (Sahoo et al 2002, Blouin et al 2011.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of the Small Heat Shock Protein, PtsHSP19.3 from Marine Red Algae, Pyropia tenera (Bangiales, Rhodophyta) Enhances Abiotic Stress Tolerance in Chlamydomonas

Jin

Yang

et al. 2017

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or H 2 O 2 . When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Ptshsp 193 Cdna Isolation and Sequence Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Overexpression of the Small Heat Shock Protein, PtsHSP19.3 from Marine Red Algae, Pyropia tenera (Bangiales, Rhodophyta) Enhances Abiotic Stress Tolerance in Chlamydomonas

Jin

Yang

et al. 2017

J Plant Biotechnol

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“…With the advent of next-generation sequencing (NGS) methods such as Roche/454 and Illumina/Hi-seq, large-scale transcriptome sequences can be generated economically from a wider range of organisms (Cheung et al 2008;Huang et al 2012;Lister et al 2008;Qin et al 2008). NGS has also been used in marine algae to determine differentially expressed genes (DEG) between different culture conditions, including abiotic stressors (Choi et al 2013;Pearson et al 2010;Yang et al 2011), and for transcriptome characterization of Pyropia and Porphyra (Chan et al 2011(Chan et al , 2012aStiller et al 2012).…”

Section: Introductionmentioning

confidence: 99%

De novo assembly of transcriptome from the gametophyte of the marine red algae Pyropia seriata and identification of abiotic stress response genes

Choi

Hwang

et al. 2014

J Appl Phycol

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Pyropia seriata, a marine red alga belonging to the order Bangiales (Rhodophyta), is one of the most valuable and widely cultivated Pyropia species. Water temperature and salinity are major factors affecting the growth and yield of Pyropia cultivation. Currently, there is limited data regarding the regulatory pathways and molecular mechanisms of the abiotic stress responses in red algae. In this study, we generated 1,403,321 expression sequence tags (ESTs) using 454 sequencing technology from the gametophyte thalli under control and high temperature conditions to identify the abiotic stress response genes. De novo assembly of the transcriptome reads generated 11,218 contigs, whereas 135,292 sequences remained as unassembled reads. Approximately 61.9 % of the contigs shared significant homology with known genes in our database, including the NCBI RefSeq database, plus Pyropia and Porphyra sequences. Sequence analyses demonstrated that the Pyropia transcriptome has a relatively high guaninecytosine (GC) content with predominant trinucleotide repeats (93.9 %). GGC was the most common motif in identified simple sequence repeats (SSRs). We selected 754 differentially expressed genes (DEGs) response to abiotic stress based on reads per kilobase per million reads (RPKM) values, many of which have no significant homology with known sequences in public database. These transcriptome sequences will facilitate future studies to understand the common processes and novel mechanisms involved in abiotic stress tolerance in red algae.

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Recent Advances in the Transcriptomics of Seaweeds

Tanna

Mishra

2020

Encyclopedia of Marine Biotechnology

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Transcriptome sequencing and comparative analysis of the gametophyte thalli of Pyropia tenera under normal and high temperature conditions

Cited by 38 publications

References 40 publications

Overexpression of the Small Heat Shock Protein, PtsHSP19.3 from Marine Red Algae, Pyropia tenera (Bangiales, Rhodophyta) Enhances Abiotic Stress Tolerance in Chlamydomonas

Overexpression of the Small Heat Shock Protein, PtsHSP19.3 from Marine Red Algae, Pyropia tenera (Bangiales, Rhodophyta) Enhances Abiotic Stress Tolerance in Chlamydomonas

De novo assembly of transcriptome from the gametophyte of the marine red algae Pyropia seriata and identification of abiotic stress response genes

Recent Advances in the Transcriptomics of Seaweeds

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