Transcriptome Sequencing and Analysis of Seabuckthorn (Hippophae Sp.)

Abstract

Seabuckthorn (Hippophae Sp.) is known for its immense medicinal, nutritional, and agronomical properties and has been utilized for centuries as traditional medicine and food supplements in various countries of Asia and Europe. The revolutionary development in the field of sequencing from first to the third generation opens a whole new horizon to explore the transcriptome of non-crop but important plant species. Many next-generation sequencing (NGS)-based transcriptome studies exploring different prospects such as therapeutic uses, stress management, and sex determination have been conducted for seabuckthorn in the last decade. Currently, a complete transcriptome profile from leaf and root tissues along with other transcriptomes profiled under different environmental conditions is available in the public domain for seabuckthorn. In the current chapter, we have highlighted the recent developments and techniques used for NGS-based transcriptome profiling for seabuckthorn. We have also discussed the challenges and prospects of NGS-based transcriptome studies in seabuckthorn. The transcriptome profiles generated for seabuckthorn could further be exploited to isolate molecular markers, microRNAs, other small and long non-coding RNAs, and gene families specific to this plant. Finally, a strategy for the utilization of third (next to next) generation sequencing to explore the seabuckthorn transcriptome is also discussed. The current chapter could serve as a good reference for transcriptome studies in seabuckthorn and other related plant species.