Transcriptome sequencing analysis of sorghum callus with various regeneration capacities

Zhou, Chao; Wang, Sijia; Zhou, Hanlin; Zhu, Yanfei; Zhou, Tao; Zhang, Yonghong; Xiang, Sen; Yang, Fang; Shen, Xiangling

doi:10.1007/s00425-021-03683-4

Cited by 8 publications

(8 citation statements)

References 51 publications

(69 reference statements)

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“…This observation further supports the findings of previous authors working on different species [98,99]. Recently, it was proven that there is a close relationship between lignin metabolic processes and the metabolism of starch and sucrose as the main factors associated with callus regeneration [100], which could be further investigated for different processes of cell differentiation. Another easily noticed difference when we analyzed the XLEs is that they were less developed in MS62 compared to the N69 mineral composition.…”

Section: Histological Analysis Of Callus: Parenchymatic Cells Full Of...supporting

confidence: 92%

De Novo Shoot Development of Tropical Plants: New Insights for Syngonium podophyllum Schott.

Sand

Antofie

2022

Horticulturae

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Syngonium podophyllum Schott. cv. ‘White Butterfly’ is recognized as a valuable ornamental plant, and today it is also an important plant species of medicinal interest due to its high contents of phenolic compounds. The purpose of this article is to review the main scientific publications from our laboratory with regard to new scientific achievements dealing with Syngonium species or topics of interest, such as callus formation and further de novo shoot regeneration. The principles and stages necessary to start an industrial-level micropropagation protocol are discussed based on our experience. Different media compositions induced different morphogenetic responses inside the callus—particularly those related to the development of xylematic elements in the organogenetic areas, such as those for rooting, protocorms, and de novo shoot formation. The re-evaluation of old histological images revealed for the first time that xylematic elements are constantly closely positioned to all organogenetic centers, and that their development is closely dependent on the composition of the culture medium. Separate protocorms can be identified only when xylematic tracheary elements are well developed and closely connected to them. The formation of protocorms is strongly dependent on the mineral composition of the culture medium and the balance of plant growth regulators.

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Section: Histological Analysis Of Callus: Parenchymatic Cells Full Of...supporting

confidence: 92%

De Novo Shoot Development of Tropical Plants: New Insights for Syngonium podophyllum Schott.

Sand

Antofie

2022

Horticulturae

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“…It plays an important role as an energy source and osmosis in the process of organogenesis (Verma and Dougall, 1977;Cui et al, 2019). Moreover, the correlation between starch metabolism and shoot regeneration has been reported in rice (Lee and Huang, 2013;Lee and Huang, 2014) and sorghum (Zhou et al, 2021). Previous studies indicated that SWEET13 and SWEET14 proteins were linked to sugar transport and involved in gibberellin-mediated physiological processes at the seedling stage of Arabidopsis (Kanno et al, 2016).…”

Section: Analysis Of Starch and Sucrose Metabolism In Callus Inductionmentioning

confidence: 99%

Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum

Shu

Altaf

et al. 2022

Front. Plant Sci.

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Regeneration is extremely important to pepper genetic development; however, the molecular mechanisms of how the callus reactivates cell proliferation and promotes cell reprogramming remain elusive in pepper. In the present study, C. baccatum (HNUCB81 and HNUCB226) and C. chinense (HNUCC22 and HNUCC16) were analyzed to reveal callus initiation by in vitro regeneration, histology, and transcriptome. We successfully established an efficient in vitro regeneration system of two cultivars to monitor the callus induction of differential genotypes, and the regenerated plants were obtained. Compared to C. chinense, there was a higher callus induction rate in C. baccatum. The phenotype of C. baccatum changed significantly and formed vascular tissue faster than C. chinense. The KEGG enrichment analysis found that plant hormone transduction and starch and sucrose metabolism pathways were significantly enriched. In addition, we identified that the WOX7 gene was significantly up-regulated in HNUCB81 and HNUCB226 than that in HNUCC22 and HNUCC16, which may be a potential function in callus formation. These results provided a promising strategy to improve the regeneration and transformation of pepper plants.

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“…Glucose metabolism, avonoid biosynthesis and Genes related to stress have been shown to be involved in the response of wheat cells to Agrobacterium tumefaciens. Many carbohydrate and amino acid metabolites were also found in maize embryo callus induced by maize immature embryos [48][49][50]. Genes related to starch/sucrose metabolism were identi ed in rice and camphor embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Genes related to starch/sucrose metabolism were identi ed in rice and camphor embryos. Furthermore, lignin metabolism occurred in sorghum callus with high regenerative capacity, carbon metabolism, amino acid biosynthesis, glycolysis/gluconeogenesis and so on were identi ed in maize somatic embryos [50][51][52].…”

Section: Discussionmentioning

confidence: 99%

Integration of Transcriptome and Metabolome Provides Unique Insights to Pathways Associated with Wheat Immature Embryos Infected by Agrobacterium tumefaciens

Wang

Guo

Jiayang

et al. 2023

Preprint

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Background: The transformation efficiency (TE) of wheat is slow due to its recalcitrance nature to in vitro regeneration and genetic transformation. Nowadays, the TE of wheat immature embryo from cultivar Fielder in the PureWheat technique was greatly improved by a series of special chemical and physical methods. To explore the mechanisms of wheat high TE in this protocol, we strictly followed the procedures to obtain wheat immature embryos after Agrobacterium infection for 24 and 48 h, observed the surface of immature embryos using scanning electron microscope (SEM), and conducted comprehensive transcriptome and metabolome analysis. Results: SEM showed that Agrobacterium tumefaciens were deposited under the damaged cortex of wheat immature embryos caused by pretreatment and contacted the receptor cells to improve the TE. Transcriptome analysis showed that the differentially expressed genes were mainly enriched in phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant-pathogen interaction, plant hormone signal transduction, MAPK signaling pathway. By analyzing the correlation between differentially expressed genes and metabolites, the expression of many genes and accumulation of metabolites are changed in glucose metabolism and the TCA cycle, as well as amino acid metabolism, it suggests that the wheat embryo infected with Agrobacterium is an energy demanding process. The shikimate pathway may act as a hub between glucose metabolism and phenylpropane metabolism during Agrobacterium infection. The down-regulation of F5H gene and up-regulation of CCR gene led to the accumulation of lignin precursor through phenylpropane metabolism. In addition, several metabolic pathways and oxidases were found to be involved in the infection treatment, including melatonin biosynthesis, benzoxazinoid biosynthesis, betaine biosynthesis, superoxide dismutase, peroxidase, which suggest that wheat embryo may be under the stress of Agrobacterium and thus undergo oxidative stress response. Conclusion: Our findings contribute to understand the underlying the mysterious mechanism of the high TE of wheat immature embryos stimulated by Agrobacteriumby exploring valuable genes and metabolites.

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Transcriptome sequencing analysis of sorghum callus with various regeneration capacities

Abstract: Main conclusionThe possible molecular mechanisms regulating sorghum callus regeneration were revealed by RNA-sequencing.

Cited by 8 publications

References 51 publications

De Novo Shoot Development of Tropical Plants: New Insights for Syngonium podophyllum Schott.

De Novo Shoot Development of Tropical Plants: New Insights for Syngonium podophyllum Schott.

Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum

Integration of Transcriptome and Metabolome Provides Unique Insights to Pathways Associated with Wheat Immature Embryos Infected by Agrobacterium tumefaciens

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