Transcriptome reveals the important role of metabolic imbalances, immune disorders and apoptosis in the treatment of Procambarus clarkii at super high temperature

Physiological and Histological Changes in Crayfish Procambarus clarkii after Acute Temperature Stress

“…HSPs are a classic stress proteins family act as molecular chaperones to reduce the misfolding of damaged proteins (Wu et al, 2011). High mRNA expression of HSP70 and HSP90 gene in the hepatopancreas was induce by high or low temperature was confirmed in our former report (Luo et al, 2021). Similarly, was found in Litopenaeus vannamei under the both short-term and longterm heat stress (Ulaje et al, 2020).…”

“…Thermal stress indeed causes gill tissues were deformed, red blood cells were necrosis, and respiratory cells were expensed in carp (Ashaf-ud-Doulah et al, 2019). Our molecular evidence also supports that high temperature treatment triggers apoptosis (Luo et al, 2021). Besides, the rupture of the gill membrane will increase the infection rate of bacteria and viruses.…”

“…(Feder and Hofmann, 1999;Wu et al, 2011), revealing a complex physiological and molecular process. Though we have shown that super high temperature (35°C) cause metabolic imbalance, immune disorders and apoptosis of P. clarkii by transcriptome, the other changes in the organisms need to be analysed (Luo et al, 2021). In order to understand the effects of acute temperature stress on the immunity, metabolism, and stress proteins and tissue structure of P. clarkii, the antioxidant, immune, metabolism-related enzyme activities and response of the hepatopancreas and gill tissues of P. clarkii under temperature stress were detected and the microstructure of hepatopancreas and gill tissue sections were observed in this study.…”

Combined effects of ration levels and temperature on immune responses of the triangle sail mussel Hyriopsis cumingii

“…Immune-related genes in the present study displayed upregulated mRNA levels in H. cumingii after cold stress, which was supposed to create an inflammatory milieu in liver tissues, and stimulate the immune response of mussels. At high temperature, decreased expression of HcIL-17 and IAP in H. cumingii showed that high temperature changed expression of immune-related genes, hence supporting that hyperthermia causes immune disorders, metabolic imbalance and triggered apoptosis at last (Luo et al, 2020). Non-specific immunity decreases after 30 days of starvation, and expression of IL-17 is reduced in hybrid grouper (Liu et al, 2020).…”

“…At high temperature, decreased expression of HcIL‐17 and IAP in H . cumingii showed that high temperature changed expression of immune‐related genes, hence supporting that hyperthermia causes immune disorders, metabolic imbalance and triggered apoptosis at last (Luo et al, 2020). Non‐specific immunity decreases after 30 days of starvation, and expression of IL‐17 is reduced in hybrid grouper (Liu et al, 2020).…”