Transcriptome responses involved in artemisinin production in Artemisia annua L. under UV-B radiation

Pan, Wei; Li, Zheng; Tian, Hao; Li, Wan Yi; Wang, Jian Wen

doi:10.1016/j.jphotobiol.2014.08.013

Cited by 59 publications

(31 citation statements)

References 46 publications

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Section: Discussionmentioning

confidence: 99%

“…Previous reports have also shown that ultraviolet (UV) light significantly induced the expression of key genes in artemisinin biosynthesis and led to an increase in the concentration of artemisinin (Rai et al, 2011; Pandey and Pandey-Rai, 2014; Pan et al, 2014). In 2014, Pan et al reported that UV-B treatment not only stimulated the biosynthesis of artemisinin, but also induced the generation of reactive oxygen species (ROS), enhanced peroxidase activity and endogenous content of abscisic acid (ABA) in the seedlings (Pan et al, 2014). Thus, they detected the transcriptomic changes during UV-B radiation (wavelength range 280–320 nm) in A. annua using an Agilent GeneChip with 43,692 probe sets and they found that the expression of AaADS, AaCYP71AV1 , and related WRKY TFs was up-regulated significantly for artemisinin biosynthesis (Pan et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In 2014, Pan et al reported that UV-B treatment not only stimulated the biosynthesis of artemisinin, but also induced the generation of reactive oxygen species (ROS), enhanced peroxidase activity and endogenous content of abscisic acid (ABA) in the seedlings (Pan et al, 2014). Thus, they detected the transcriptomic changes during UV-B radiation (wavelength range 280–320 nm) in A. annua using an Agilent GeneChip with 43,692 probe sets and they found that the expression of AaADS, AaCYP71AV1 , and related WRKY TFs was up-regulated significantly for artemisinin biosynthesis (Pan et al, 2014). In our study, we also found that the expression of artemisinin biosynthetic genes was influenced by the light and they all reduced in the dark for 24 h relative to the expression level under the light.…”

Section: Discussionmentioning

confidence: 99%

“…A. annua is a famous traditional Chinese medicinal plant which is the main source of the antimalarial drug artemisinin. There are some reports on transcript profiling and transcriptome sequencing of the A. annua before (Wang et al, 2009; Graham et al, 2010; Maes et al, 2011; Soetaert et al, 2013; Pan et al, 2014). In our study, we found that the improvement of the MeJA to the artemisinin biosynthesis is dependent on the light.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis of Genes Associated with the Artemisinin Biosynthesis by Jasmonic Acid Treatment under the Light in Artemisia annua

Hao

Zhong

et al. 2017

Front. Plant Sci.

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Artemisinin is a sesquiterpene lactone endoperoxide extracted from a traditional Chinese medicinal plant Artemisia annua. Artemisinin-based combination therapies (ACTs) are recommended as the best treatment of malaria by the World Health Organization (WHO). Both the phytohormone jasmonic acid (JA) and light promote artemisinin biosynthesis in A. annua. Interestingly, we found that the increase of artemisinin biosynthesis by JA was dependent on light. However, the relationship between the two signal pathways mediated by JA and light remains unclear. Here, we collected the A. annua seedlings of 24 h continuous light (Light), 24 h dark treatment (Dark), 4 h MeJA treatment under the continuous light conditions (Light-MeJA-4h) and 4 h MeJA treatment under the dark conditions (Dark-MeJA-4h) and performed the transcriptome sequencing using Illumina HiSeq 4000 System. A total of 266.7 million clean data were produced and assembled into 185,653 unigenes, with an average length of 537 bp. Among them, 59,490 unigenes were annotated and classified based on the public information. Differential expression analyses were performed between Light and Dark, Light and Light-MeJA-4h, Dark and Dark-MeJA-4h, Light-MeJA-4h, and Dark-MeJA-4h, respectively. Furthermore, transcription factor (TF) analysis revealed that 1588 TFs were identified and divided into 55 TF families, with 284 TFs down-regulated in the Dark relative to Light and 96 TFs up-regulated in the Light-MeJA-4h relative to Light. 8 TFs were selected as candidates for regulating the artemisinin biosynthesis and one of them was validated to be involved in artemisinin transcriptional regulation by Dual-Luciferase (Dual-LUC) assay. The transcriptome data shown in our study offered a comprehensive transcriptional expression pattern influenced by the MeJA and light in A. annua seedling, which will serve as a valuable resource for further studies on transcriptional regulation mechanisms underlying artemisinin biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome Analysis of Genes Associated with the Artemisinin Biosynthesis by Jasmonic Acid Treatment under the Light in Artemisia annua

Hao

Zhong

et al. 2017

Front. Plant Sci.

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“…On such basis, the biotechnological method elicitation which mimics the natural induction of plant stress is the method of choice to enhance the production of artemisinin. The positive effects of UV-B and DMSO on the production of artemisinin had been verified by previous studies on A. annua seedlings [28, 29], in vitro propagated plantlets [30] and shoot cultures [31]. Former studies had also shown that artemisinin production can be enhanced by different elicitors in A. annua cell cultures [32–35].…”

Section: Introductionmentioning

confidence: 56%

Successes of artemisinin elicitation in low-artemisinin producing Artemisia annua cell cultures constrained by repression of biosynthetic genes

Yee

Ping

2019

Preprint

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10The sesquiterpene phytolactone derived from Artemisia annua, artemisinin is 11 associated with a variety of novel biological properties, such as immunoregulatory and 12 anticancer effects, and therapeutic applications, apart from its main function as an 13 4 57 Naturally, artemisinin biosynthesis occurs in glandular secretory trichomes that are 58 present on foliage, stems and inflorescences of the A. annua plant [15, 16]. Due to its 59 compartmentalized synthesis and influence by physiological, seasonal and environmental 60 factors, artemisinin concentration is highly variable with reported values of 0.1 -10 mg g -1 61 dry weight (DW) [11, 17]. With the biosynthetic pathway of artemisinin formation fully 62 elucidated over the past decade, it was learnt that within the species A. annua two contrasting 63 chemotypes can be distinguished, which are characterized by the differing contents of 64 artemisinin and its precursor (Fig 1). While both possess artemisinin, the high-artemisinin 65 producing (HAP) chemotype contains relatively higher levels of dihydroartemisinic acid 66 (DHAA) and artemisinin, and the low-artemisinin producing (LAP) chemotype has higher 67 contents of artemisinic acid and arteannuin B (a non-antimalarial product) [18, 19]. Despite 68 the difference in biochemical phenotype which is attributed to the differential expression of 69 one artemisinin biosynthesis specific gene artemisinic aldehyde Δ11(13) reductase (DBR2), 70 both chemotypes undergo similar spontaneous photooxidation reactions to produce 71 artemisinin or arteannuin B from its direct precursors [20-23]. 72 Fig 1. Schematic representation of artemisinin biosynthesis. 73 Synthesis occurs in the glandular trichome secretory cells of A. annua and sequestration of 74 artemisinin takes place in the subcuticular space. The two branching pathways leading to 75 artemisinin and arteannuin B of the artemisinin biosynthetic pathway (dashed line box) are 76 depicted and the final non-enzymatic photo-oxidation reaction is shown in red. MEP, 77 methylerythritol 4-phosphate; MVA, mevalonate; GST, glandular secretory trichome; ADS, 78 amorpha-4,11-diene synthase; CYP71AV1, cytochrome P450 monooxygenase; DBR2, 79 artemisinic aldehyde Δ11(13) reductase; ALDH1, aldehyde dehydrogenase 1. 80 As a secondary metabolite used mainly in defence mechanisms, production of 81 artemisinin would naturally be induced and enhanced under environmental stresses. Under 5 82 such adverse conditions the elevation of secondary oxidative stress, which is a consequence 83 of primary biotic/abiotic stress, is in a direct synergistic relationship with artemisinin 84 production [24-27]. On such basis, the biotechnological method elicitation which mimics the 85 natural induction of plant stress is the method of choice to enhance the production of 86 artemisinin. The positive effects of UV-B and DMSO on the production of artemisinin had 87 been verified by previous studies on A. annua seedlings [28, 29], in vitro propagated plantlets 88 [30] and shoot cultures [31]. Former st...

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Impact of Abiotic Stresses on Production of Secondary Metabolites in Medicinal and Aromatic Plants

Jampílek,

Kráľová

2023

Environmental Science and Engineering

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Transcriptome responses involved in artemisinin production in Artemisia annua L. under UV-B radiation

Cited by 59 publications

References 46 publications

Transcriptome Analysis of Genes Associated with the Artemisinin Biosynthesis by Jasmonic Acid Treatment under the Light in Artemisia annua

Transcriptome Analysis of Genes Associated with the Artemisinin Biosynthesis by Jasmonic Acid Treatment under the Light in Artemisia annua

Successes of artemisinin elicitation in low-artemisinin producing Artemisia annua cell cultures constrained by repression of biosynthetic genes

Impact of Abiotic Stresses on Production of Secondary Metabolites in Medicinal and Aromatic Plants

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