Transcriptome regulation of extracellular vesicles derived from porcine uterine flushing fluids during peri-implantation on endometrial epithelial cells and embryonic trophoblast cells

Hua, Renwu; Liu, Qiaorui; Lian, Weisi; Gao, Dengying; Lei, Minggang

doi:10.1016/j.gene.2022.146337

Cited by 9 publications

(7 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ULF-EVs labeled by PKH67 have been reported to be observed in endometrial epithelial cells and PTr2 cells [ 33 ]. In addition, EVs derived from embryonic trophoblast cells were found to act on endometrial epithelial cells to promote embryo implantation [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Ding

Gan

et al. 2022

Animals

View full text Add to dashboard Cite

Extracellular vesicles (EVs) in uterine luminal fluid (ULF) can reportedly affect the proliferation and migration function of porcine trophoblast cells (PTr2 cells) by mediating the maternal–fetal exchange of information. miR-143-3p is considered a crucial miRNA in early pregnancy in mammals; however, little is currently known about how it regulates the function of PTr2 cells. This study aimed to investigate the effects of ssc-miR-143-3p in ULF-EVs on the function of PTr2 cells during porcine embryo implantation. The uptake of ULF-EVs by PTr2 cells was confirmed, which significantly increased the expression of ssc-miR-143-3p. Ssc-miR-143-3p was found to facilitate the proliferation and migration of PTr2 cells in the CCK-8, EdU and wound-closure assays, while the opposite findings were observed after the knockdown of ssc-miR-143-3p. Bioinformatics analysis and the luciferase reporter assay showed that glycerol-3 phosphate dehydrogenase 2 (GDP2) was directly targeted by miR-143-3p. Inhibition of miR-143-3p was validated in mice to inhibit embryo implantation. In summary, ssc-miR-143-3p in ULF-EVs affects the proliferation and migration of PTr2 cells by mediating GPD2, thereby affecting embryo implantation.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The particles in ULF were separated by centrifugation at different speeds [ 33 ]. The supernatant was collected by centrifugation at 2000× g for 20 min to remove cells and blood and at 10,000× g for 30 min to remove cell debris, large granular vesicles and apoptotic vesicles.…”

Section: Methodsmentioning

confidence: 99%

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Ding

Gan

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…The study described, identified, and characterized different types of RNAs in EVs during different stages of gestation and revealed how some of these RNAs were related to important processes such as immunization, endometrial receptivity, and embryonic development. Hua et al [ 162 ] focused on the importance of interactions between signaling molecules in the uterus and the porcine conceptus for successful implantation. They described how EVs in the uterine luminal fluid play a role in conceptus development and how these EVs are primarily derived from endometrial epithelial cells.…”

Section: Extracellular Vesicles and Mirna In Pig Gestationmentioning

confidence: 99%

Changes in Immune Response during Pig Gestation with a Focus on Cytokines

Velez,

Williamson,

Cánovas

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

Pigs have the highest percentage of embryonic death not associated with specific diseases of all livestock species, at 20–45%. During gestation processes, a series of complex alterations can arise, including embryonic migration and elongation, maternal immunological recognition of pregnancy, and embryonic competition for implantation sites and subsequent nutrition requirements and development. Immune cells and cytokines act as mediators between other molecules in highly complex interactions between various cell types. However, other non-immune cells, such as trophoblast cells, are important in immune pregnancy regulation. Numerous studies have shed light on the crucial roles of several cytokines that regulate the inflammatory processes that characterize the interface between the fetus and the mother throughout normal porcine gestation, but most of these reports are limited to the implantational and peri-implantational periods. Increase in some proinflammatory cytokines have been found in other gestational periods, such as placental remodeling. Porcine immune changes during delivery have not been studied as deeply as in other species. This review details some of the immune system cells actively involved in the fetomaternal interface during porcine gestation, as well as the principal cells, cytokines, and molecules, such as antibodies, that play crucial roles in sow pregnancy, both in early and mid-to-late gestation.

show abstract

“…EVs present in the uterine lumen on Day 13 of pregnancy, delivered in vitro to both epithelial endometrial (Day 12 of the estrous cycle) and trophoblast cells (PTr2 cell line; origin—Day 12 filamentous conceptus), altered the expression of thousands of genes in recipient cells. Enrichment analysis indicated that these genes are involved in immune system regulation (CCL family, e.g., CCL1 , CCL2 , and CXCL family, e.g., CXCL10 , CXLCL11 ), cellular processes such as migration (MMPs, e.g., MMP9 , MMP11 , and TIMPs, e.g., TIMP1 and TIMP3 ) and adhesion (integrin α genes, e.g., ITGA2 , ITGA5 , integrin β genes, e.g., ITGB3 , ITGB5 , cadherin genes, e.g., CDH1 , CDH3 ), as well as EV secretion and uptake ( TSG101 and MX1 ) (Hua et al, 2022). Other studies have shown that uterine EVs derived from Day 15 pregnant pigs inhibit migration and proliferation of immortalized Day 12 porcine trophoblast cells (Hu et al, 2022).…”

Section: Evs As Messengers In Early Pregnancy Eventsmentioning

confidence: 99%

Extracellular vesicles: Focus on peri‐implantation period of pregnancy in pigs

Guzewska

Szuszkiewicz

Kaczmarek

2023

Molecular Reproduction Devel

View full text Add to dashboard Cite

The establishment of cell‐to‐cell communication between the endometrium and the developing embryo is the most important step in successful mammalian pregnancy. Close interaction between the uterine luminal epithelium and trophoblast cells requires triggering timely molecular dialog for successful maternal recognition of pregnancy, embryo implantation, and placenta development. Quite recently, extracellular vesicles (EVs) carrying unique molecular cargo emerged as evolutionarily conserved mediators of cell‐to‐cell communication during early pregnancy. To date, the presence of EVs at the embryo–maternal interface has been demonstrated in numerous mammals, including domestic livestock, such as pigs. However, few studies have focused on revealing the mechanism of EV‐mediated crosstalk between developing early embryos and receptive endometrium. Over the past years, it has appeared that understanding the role of EVs in mammalian reproduction can substantially improve our understanding of the biological challenges of successful reproductive performance. This review describes current knowledge of EVs, specifically in relation to the peri‐implantation period in pigs, characterized by common features of embryo implantation and high embryonic mortality in mammals.

show abstract

Transcriptome regulation of extracellular vesicles derived from porcine uterine flushing fluids during peri-implantation on endometrial epithelial cells and embryonic trophoblast cells

Cited by 9 publications

References 57 publications

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Changes in Immune Response during Pig Gestation with a Focus on Cytokines

Extracellular vesicles: Focus on peri‐implantation period of pregnancy in pigs

Contact Info

Product

Resources

About