Transcriptome/Proteome Analysis of Corynebacterium glutamicum

Wendisch, Volker F.; Polen, Tino

doi:10.1007/978-3-642-29857-8_6

Cited by 3 publications

(1 citation statement)

References 210 publications

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“…Beech wood (Demirbas, 2007) as the sole carbon source for microbial growth (Collins & Kornberg, 1960;Gerstmeir, Cramer, Dangel, Schaffer, & Eikmanns, 2004;Hayashi et al, 2002;Kornberg, 1966;Kornberg & Madsen, 1958;Reinscheid, Eikmanns, & Sahm, 1994a, 1994bWendisch, Graaf, Sahm, & Eikmanns, 2000;Wendisch et al, 1997). However, many bacteria lack the key glyoxylate cycle enzyme ICL.…”

Section: Acetic Acid Formic Acid Propionic Acidmentioning

confidence: 99%

Evaluation of small organic acids present in fast pyrolysis bio‐oil from lignocellulose as feedstocks for bacterial bioconversion

et al. 2019

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Small organic acids derived from fast pyrolysis of lignocellulosic biomass represent a significant proportion of microbially accessible carbon in bio‐oil. However, using bio‐oil for microbial cultivation is a highly challenging task due to its strong adverse effects on microbial growth as well as its complex composition. In this study, the main small organic acids present in bio‐oil as acetate, formate and propionate were evaluated with respect to their suitability as feedstocks for bacterial growth. For this purpose, the growth behavior of four biotechnological production hosts—Escherichia coli, Pseudomonas putida, Bacillus subtilis, and Corynebacterium glutamicum—was quantified and compared. The bacteria were cultivated on single acids and mixtures of acids in different concentrations and evaluated using common biotechnological efficiency parameters. In addition, cultivation experiments on pretreated fast pyrolysis‐derived bio‐oil fractions were performed with respect to the suitability of the bacterial strains to tolerate inhibitory substances. Results suggest that both P. putida and C. glutamicum metabolize acetate—the major small organic acid generated during fast pyrolysis of lignocellulosic biomass—as sole carbon source over a wide concentration range, are able to grow on mixtures of small organic acids present in bio‐oil and can, to a limited extent, tolerate the highly toxic inhibitory substances within bio‐oil. This work provides an important step in search of suitable bacterial strains for bioconversion of lignocellulosic‐based feedstocks and thus contributes to establishing efficient bioprocesses within a future bioeconomy.

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