Transcriptome profiling reveals the occurrence mechanism of bisexual flowers in melon (Cucumis melo L.)

Ge, Chutian; Zhao, Wensheng; Niu, Shan-Ce; Fang, Siyu; Yong, Duan; Zhao, Jiateng; Guo, Kedong; Zhang, Qian

doi:10.1016/j.plantsci.2020.110694

Cited by 12 publications

(21 citation statements)

References 45 publications

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“…Exogenous ethephon treatment of melon plants were performed as previously described ( Ge et al, 2020 ). During the development period of 4–6 internodes, ethephon treatment was performed and 5 mL of 200 ppm ethephon dissolving in water was uniformly sprayed on the shoot apex of main stem by the atomizer.…”

Section: Methodsmentioning

confidence: 99%

“…CmACS11 can repress the expression of CmWIP1 and the plants with loss-of-function of CmACS11 were transformed into androecy ( Boualem et al, 2015 ). In addition, plentiful regulators related to “plant hormone signal transduction”, “MAPK signaling pathway” and “carbon metabolism” were found to different expressed during the process of the occurrence of bisexual flowers ( Ge et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Distinct metabolic profiling is correlated with bisexual flowers formation resulting from exogenous ethephon induction in melon (Cucumis melo L.)

Fang

Yong

Zhao

et al. 2022

PeerJ

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Melon (Cucumis melo L.) is an agronomically important vegetable. Most cultivars of melon are andromonoecious and bisexual flowers only emerged from the leaf axil of lateral branches. However, the regulatory mechanism contributing to the occurrence of bisexual flowers were still obscure. In this study, ethephon was applied in two common cultivars of melon. In control without ethephon treatment, no bisexual flower was made in the main stem. However, 6.56 ± 1.42 and 6.63 ± 0.55 bisexual flowers were respectively induced in main stem of ‘Yangjiaocui-QX’ and ‘Lvbao’ after ethephon treatment, and induced bisexual flowers distributed in 12–20 nodes of main stem. During the formation of bisexual flowers, 41 metabolites were significantly up-regulated and 98 metabolites were significantly down-regulated. According to the KEGG enrichment analysis of 139 different metabolites, a total of 30 pathways were mapped and KEGG terms of “Phenylalanine, tyrosine and tryptophan biosynthesis”, “Phenylalanine metabolism” and “Flavone and flavonol biosynthesis” were significantly enriched. In three significantly enriched KEGG terms, shikimic acid, L-tryptophan, L-phenylalanine, and kaempferol were significantly up-regulated while L-tyrosine, 4-hydroxycinnami acid and luteolin were significantly down-regulated in ET compared to CK. Different metabolites were also classified depend on major class features and 14 classes were acquired. The results of metabonomics and endogenous hormone identification indicated that ethylene could enhance the concentration of salicylic acid, methyl jasmonate, ABA and IAA. This study provided an important theoretical foundation for inducing bisexual flowers in main stem and breeding new varieties of melon in future.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Distinct metabolic profiling is correlated with bisexual flowers formation resulting from exogenous ethephon induction in melon (Cucumis melo L.)

Fang

Yong

Zhao

et al. 2022

PeerJ

Self Cite

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“…Moreover, the GA has a higher tendency for promoting maleness in the andromonoecious cucumber and could regulate the sex- ethephon treated samples and therefore, identified the molecular mechanism of bisexual flowers in melons (Ge et al, 2020). However, CmERF1 showed a significant difference in the expression pattern and had a higher expression in bisexual buds rather than male buds (Ge et al, 2020).…”

Section: Protein-protein Interaction Networkmentioning

confidence: 96%

“…Generally, commercially grown melon is andromonoecious where male flowers develop on both the main stem as well as on the lateral branches but bisexual flowers only emerged from the leaf axils of lateral branches on the same plant through a well-determined developmental gradient (Papadopoulou & Grumet, 2005;Grumet et al, 2007;Girek et al, 2013;Gao et al, 2015;Latrasse et al, 2017;Revanasidda & Belavadi, 2019;Ge et al, 2020). Additionally, recessive mutants develop the bisexual or female flowers at every node have been identified as hermaphrodite and gynoecious sex phenotypes, respectively, in melon (Abdelmohsin et al, 2015;Boualem et al, 2015;Schaffer & Paris, 2003).…”

mentioning

confidence: 99%

“…Recently, Ge et al (2020) performed the transcriptomic studies to unravel the occurrence of bisexual flower mechanism in the melon (C. melo L.) (Ge et al, 2020). The latest research findings related to sex determination, sex expression, potential genes involved, and the molecular pathways regulating the sex expression in melon and other cucurbits have been summarized in Table 1.…”

mentioning

confidence: 99%

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A novel insight into transcriptional and epigenetic regulation underlying sex expression and flower development in melon (Cucumis melo L.)

Aamir

Karmakar

Singh

et al. 2021

Physiologia Plantarum

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Melon (Cucumis melo L.) is an important cucurbit and has been considered as a model plant for studying sex determination. The four most common sexual morphotypes in melon are monoecious (A-G-M), gynoecious (−-ggM-), andromonoecious (A-G-mm), and hermaphrodite (--ggmm). Sex expression in melons is complex, as the genes and associated networks that govern the sex expression are not fully explored. Recently, RNA-seq transcriptomic profiling, ChIP-qPCR analysis integrated with gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathways predicted the differentially expressed genes including sex-specific ACS and ACO genes, in regulating the sex-expression, phytohormonal cross-talk, signal transduction, and secondary metabolism in melons. Integration of transcriptional control through genetic interaction in between the ACS7, ACS11, and WIP1 in epistatic or hypostatic manner, along with the recruitment of H3K9ac and H3K27me3, epigenetically, overall determine sex expression. Alignment of protein sequences for establishing phylogenetic evolution, motif comparison, and protein-protein interaction supported the structural conservation while presence of the conserved hydrophilic and charged residues across the diverged evolutionary group predicted the functional conservation of the ACS protein. Presence of the putative cis-binding elements or DNA motifs, and its further comparison with DAP-seq-based cistrome and epicistrome of Arabidopsis, unraveled strong ancestry of melons with Arabidopsis. Motif comparison analysis also characterized putative genes and transcription factors involved in ethylene biosynthesis, signal transduction, and hormonal cross-talk related to sex expression. Overall, we have comprehensively reviewed research findings for a deeper insight into transcriptional and epigenetic regulation of sex expression and flower development in melons. | INTRODUCTIONMelon (Cucumis melo L.), which belongs to the family Cucurbitaceae, is a highly diversified (fruit type and other related attributes) and important vegetable crop, cultivated worldwide for its sweet taste, and distinct flavor and aroma (Girek et al., 2013;Revanasidda & Belavadi, 2019). Apart from its economic value, C. melo has been regarded as a model cucurbit and is widely used for studying sex expression (Girek

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CmERF1 acts as a positive regulator of fruits and leaves growth in melon (Cucumis melo L.)

Sun,

Yang,

Ren

et al. 2024

Plant Mol Biol

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Transcriptome profiling reveals the occurrence mechanism of bisexual flowers in melon (Cucumis melo L.)

Cited by 12 publications

References 45 publications

Distinct metabolic profiling is correlated with bisexual flowers formation resulting from exogenous ethephon induction in melon (Cucumis melo L.)

Distinct metabolic profiling is correlated with bisexual flowers formation resulting from exogenous ethephon induction in melon (Cucumis melo L.)

A novel insight into transcriptional and epigenetic regulation underlying sex expression and flower development in melon (Cucumis melo L.)

CmERF1 acts as a positive regulator of fruits and leaves growth in melon (Cucumis melo L.)

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