Transcriptome profiling reveals the immune response of goose T cells under selenium stimuli

Cao, Nan; Li, Wanyan; Li, Bingxin; Tian, Yunbo; Xu, Danning

doi:10.1111/asj.12861

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…It has antioxidant properties [122] and serves in homeostasis and distribution of selenium [123]. In birds, selenoprotein has been shown to be important in immune responses [124] and to be protective against growth inhibition, including nutritional muscular dystrophy [125], as well as oxidative damage and apoptosis in response to fluorine [126]. Phylogenetically, Sepp1 is believed to have appeared in early metazoan species [127].…”

Section: Discussionmentioning

confidence: 99%

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Phillips

Kraev

Lange

2020

Biology

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Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the identification of biomarkers for the assessment of their health status is of considerable importance. Peptidylarginine deiminases (PADs) post-translationally convert arginine into citrulline in target proteins in an irreversible manner. PAD-mediated deimination can cause structural and functional changes in target proteins, allowing for protein moonlighting in physiological and pathophysiological processes. PADs furthermore contribute to the release of extracellular vesicles (EVs), which play important roles in cellular communication. In the present study, post-translationally deiminated protein and EV profiles of plasma were assessed in eight seabird species from the Antarctic, representing two avian orders: Procellariiformes (albatrosses and petrels) and Charadriiformes (waders, auks, gulls and skuas). We report some differences between the species assessed, with the narrowest EV profiles of 50–200 nm in the northern giant petrel Macronectes halli, and the highest abundance of larger 250–500 nm EVs in the brown skua Stercorarius antarcticus. The seabird EVs were positive for phylogenetically conserved EV markers and showed characteristic EV morphology. Post-translational deimination was identified in a range of key plasma proteins critical for immune response and metabolic pathways in three of the bird species under study; the wandering albatross Diomedea exulans, south polar skua Stercorarius maccormicki and northern giant petrel. Some differences in Gene Ontology (GO) biological and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for deiminated proteins were observed between these three species. This indicates that target proteins for deimination may differ, potentially contributing to a range of physiological functions relating to metabolism and immune response, as well as to key defence mechanisms. PAD protein homologues were identified in the seabird plasma by Western blotting via cross-reaction with human PAD antibodies, at an expected 75 kDa size. This is the first study to profile EVs and to identify deiminated proteins as putative novel plasma biomarkers in Antarctic seabirds. These biomarkers may be further refined to become useful indicators of physiological and immunological status in seabirds—many of which are globally threatened.

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Section: Discussionmentioning

confidence: 99%

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Phillips

Kraev

Lange

2020

Biology

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“…Spleens were collected in the four groups and the procedure of isolation of lymphocytes was similar to the one published by Cao [ 29 ]. PHA (15 μg·mL −1 ; Sigma, St. Louis, MO, USA) and LPS (25 μg·mL −1 ; Sigma, St. Louis, MO, USA) were added into the culture medium to activate T-cell and B-cell proliferation, respectively.…”

Section: Methodsmentioning

confidence: 99%

Polysaccharide of Atractylodes macrocephala Koidz (PAMK) Relieves Immunosuppression in Cyclophosphamide-Treated Geese by Maintaining a Humoral and Cellular Immune Balance

Guo

et al. 2018

Molecules

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Polysaccharide of Atractylodes macrocephala Koidz (PAMK) has been well recognized as an immune enhancer that can promote lymphocyte proliferation and activate immune cells. The purpose of this study was to evaluate the effects of PAMK on humoral and cellular immune functions in immunosuppressed geese. Geese of the Control group were provided with normal feed, the PAMK group was provided with 400 mg·(kg body weight)−1 PAMK, the cyclophosphamide (CTX) group was injected with 40 mg·(kg body weight)−1 cyclophosphamide, while the CTX+PAMK group received the combination of PAMK and CTX. Spleen development and percentages of leukocytes in peripheral blood were examined. Principal component analysis was conducted to analyze correlations among humoral and cellular immune indicators. The results showed that PAMK alleviated the damage to the spleen, the decrease in T- and B-cell proliferation, the imbalance of leukocytes, and the disturbances of humoral and cellular immunity caused by CTX. Principal component analysis revealed that the relevance of humoral-immunity-related indicators was greater, and the CTX+PAMK group manifested the largest difference from the CTX group but was close to the Control group. In conclusion, PAMK alleviates the immunosuppression caused by CTX in geese, and the protective effect on humoral immunity is more obvious and stable.

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“…Chung et al 2015;Mueller et al 2015). Wright et al (2014) [3] {18, 22, 23}; Harrison et al (2015) [3] {18, 22, 23}; Li et al (2015) [2] {3, 22, 24}; Smith et al (2015) [2] {12, 16}; Wright et al (2015) [3] {18, 22, 23} Anser cygnoides Swan Goose a Harrison et al (2015) [3] {18, 22, 23}; Lu et al (2015) [1, 2] {15}; Tariq et al (2015) [2] {28}; Wright et al (2015) [3] {18, 22, 23}; Chen et al (2017b) [2] {28}; Cao et al (2017) Harrison et al (2015) [3] {18, 22, 23}; Wright et al (2014) [3] {18, 22, 23}; Wright et al (2015) [3] {18, 22, 23} Numida meleagris Helmeted Guineafowl Harrison et al (2015) [3] {18, 22, 23}; Wright et al (2015) [3] {18, 22, 23} Pavo cristatus Indian Peafowl Harrison et al (2015) [3] {18, 22, 23}; Wright et al (2015) [3] {18, 22, 23}…”

Section: Characterisation Of Transcriptomesmentioning

confidence: 99%

Avian transcriptomics: opportunities and challenges

Jax

Kraus

2018

J Ornithol

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Recent developments in next-generation sequencing technologies have greatly facilitated the study of whole transcriptomes in model and non-model species. Studying the transcriptome and how it changes across a variety of biological conditions has had major implications for our understanding of how the genome is regulated in different contexts, and how to interpret adaptations and the phenotype of an organism. The aim of this review is to highlight the potential of these new technologies for the study of avian transcriptomics, and to summarise how transcriptomics has been applied in ornithology. A total of 81 peer-reviewed scientific articles that used transcriptomics to answer questions within a broad range of study areas in birds are used as examples throughout the review. We further provide a quick guide to highlight the most important points which need to be take into account when planning a transcriptomic study in birds, and discuss how researchers with little background in molecular biology can avoid potential pitfalls. Suggestions for further reading are supplied throughout. We also discuss possible future developments in the technology platforms used for ribonucleic acid sequencing. By summarising how these novel technologies can be used to answer questions that have long been asked by ornithologists, we hope to bridge the gap between traditional ornithology and genomics, and to stimulate more interdisciplinary research.

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Transcriptome profiling reveals the immune response of goose T cells under selenium stimuli

Cited by 7 publications

References 32 publications

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Polysaccharide of Atractylodes macrocephala Koidz (PAMK) Relieves Immunosuppression in Cyclophosphamide-Treated Geese by Maintaining a Humoral and Cellular Immune Balance

Avian transcriptomics: opportunities and challenges

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