Transcriptome profiling reveals response genes for downy mildew resistance in cucumber

Gao, Xinbin; Guo, Pei; Wang, Zhiyuan; Chen, Chunhua; Ren, Zhonghai

doi:10.1007/s00425-021-03603-6

Cited by 14 publications

(16 citation statements)

References 64 publications

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“…The production of cellular ROS, including H 2 O 2 and superoxide anion, can be induced by pathogen invasion [18]. In plants, lignin plays important role in resistance to pathogen infection [15]. In the present study, lignin and H 2 O 2 significantly accumulated at the early stages of P. cubensis infection in DADS treated cucumbers (Figure 2B,C).…”

Section: Dads Induced H 2 O 2 and Lignin Response To P Cubensis Infectionsupporting

confidence: 56%

“…In the present study, lignin and H 2 O 2 significantly accumulated at the early stages of P. cubensis infection in DADS treated cucumbers (Figure 2B,C). The H 2 O 2 accumulation might be balanced by its increased synthesis or decreased degradation [15]. For H 2 O 2 , its biosynthesis is mainly controlled by SOD, while its degradation is primarily controlled by POD and CAT.…”

Section: Dads Induced H 2 O 2 and Lignin Response To P Cubensis Infectionmentioning

confidence: 99%

“…Although some resistant germplasms have been identified, the regulating genes and the pathways involved in mediating downy mildew resistance in cucumber are still unclear [14]. Several quantitative trait loci associated with downy mildew resistance have been mapped onto five of the seven cucumber chromosomes, suggesting that downy mildew resistance in cucumber is likely due to the associative effects of multiple genes [15]. Recently, CsSGR was reported as a major-effect resistant gene for cucumber downy mildew.…”

Section: Introductionmentioning

confidence: 99%

“…In cucumber, transcriptome data revealed that resistance to downy mildew is associated with an earlier response to the pathogen, hormone signaling, and regulation of nutrient supply [14]. Additionally, the pathways of pathogen-associated molecular pattern (PAMP) recognition, signal transduction, ROS and lignin accumulation, and some transcription regulators are all involved in the resistance of cucumber to downy mildew [15]. Although some research on germplasm identification, regulatory genes, and pathways of cucumber resistance to downy mildew were performed, studies on the use of allelopathic substances to induce cucumber resistance to downy mildew are rarely reported, and its potential regulatory mechanism is also unknown.…”

Section: Introductionmentioning

confidence: 99%

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Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew

Yang

Wang

Zhi

et al. 2021

IJMS

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Allicin compositions in garlic are used widely as fungicides in modern agriculture, in which diallyl disulfide (DADS) is a major compound. Downy mildew, caused by Pseudoperonospora cubensis (P. cubensis), is one of the most destructive diseases and causes severe yield losses in cucumbers. To explore the potential mechanism of DADS-induced cucumber resistance to downy mildew, cucumber seedlings were treated with DADS and then inoculated with P. cubensis at a 10-day interval. Symptom observation showed that DADS significantly induced cucumber resistance to downy mildew. Furthermore, both lignin and H2O2 were significantly increased by DADS treatment to responding P. cubensis infection. Simultaneously, the enzyme activities of peroxidase (POD) in DADS-treated seedlings were significantly promoted. Meanwhile, both the auxin (IAA) and salicylic acid (SA) contents were increased, and their related differentially expressed genes (DEGs) were up-regulated when treated with DADS. Transcriptome profiling showed that many DEGs were involved in the biological processes of defense responses, in which DEGs on the pathways of ‘phenylpropanoid biosynthesis’, ‘phenylalanine metabolism’, ‘MAPK signaling’, and ‘plant hormone signal transduction’ were significantly up-regulated in DADS-treated cucumbers uninoculated with the pathogen. Based on the results of several physiological indices and transcriptomes, a potential molecular mechanism of DADS-induced cucumber resistance to downy mildew was proposed and discussed. The results of this study might give new insight into the exploration of the induced resistance mechanism of cucumber to downy mildew and provide useful information for the subsequent mining of resistance genes in cucumber.

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Section: Dads Induced H 2 O 2 and Lignin Response To P Cubensis Infectionsupporting

confidence: 56%

Section: Dads Induced H 2 O 2 and Lignin Response To P Cubensis Infectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew

Yang

Wang

Zhi

et al. 2021

IJMS

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“…In cucumber, DM caused by P. cubensis is a serious disease that results in severe damage to the production of cucumber around the world [41,42]. We then analyzed the expression of CsGLP genes to explore their possible roles in response to DM infection.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Germin-like Proteins (GLPs) and Their Expression in Response to Abiotic and Biotic Stresses in Cucumber

Liao

Liu

et al. 2021

Horticulturae

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Germins and germin-like proteins (GLPs) are glycoproteins closely associated with plant development and stress response in the plant kingdom. Here, we carried out genome-wide identification and expression analysis of the GLP gene family in cucumber to study their possible functions. A total of 38 GLP genes were identified in cucumber, which could be mapped to six out of the seven cucumber chromosomes. A phylogenetic analysis of the GLP members from cucumber, Arabidopsis and rice showed that these GLPs could be divided into six groups, and cucumber GLPs in the same group had highly similar conserved motif distribution and gene structure. Gene duplication analysis revealed that six cucumber GLP genes were located in the segmental duplication regions of cucumber chromosomes, while 14 genes were associated with tandem duplications. Tissue expression profiles of cucumber GLP genes showed that many genes were preferentially expressed in specific tissues. In addition, some cucumber GLP genes were differentially expressed under salt, drought and ABA treatments, as well as under DM inoculation. Our results provide important information for the functional identification of GLP genes in the growth, development and stress response of cucumber.

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