Transcriptome profiling reveals genes involved in spine development during CsTTG1-regulated pathway in cucumber (Cucumis sativus L.)

Guo, Pei; Chang, Hualin; Li, Qiang; Wang, Lina; Ren, Zhonghai; Ren, Huazhong; Chen, Chunhua

doi:10.1016/j.plantsci.2019.110354

Cited by 16 publications

(11 citation statements)

References 54 publications

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“…Besides, XTH23 show different treads. The protein sequences of XTH23 in pepper [ 46 ], cucumber [ 47 ], Arabidopsis [ 48 ] and C. oleifera of these species were aligned to construct a phylogenetic tree (electronic supplementary material, figure S1). XTH23-1 in C. oleifera was similar to CaXTH23 .…”

Section: Discussionmentioning

confidence: 99%

“…In pepper, CaXTH23 was highly expressed in the early stages of fruit development and then decreased gradually with fruit ripening [ 46 ], which was consistent with the expression trend of XTH23-1 . XTH23-3 was a close relative of CsXTH23 , which was related to the rapid expansion of cells in cucumber fruit spines [ 47 ]. In C. oleifera seeds , the expression of XTH23-3 increased 420-fold in the T4 period compared with the T1 period in C. oleifera seeds .…”

Section: Discussionmentioning

confidence: 99%

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Hormone analysis and candidate genes identification associated with seed size in Camellia oleifera

Song

et al. 2022

R. Soc. open sci.

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Camellia oleifera is an important woody oil species in China. Its seed oil has been widely used as a cooking oil. Seed size is a crucial factor influencing the yield of seed oil. In this study, the horizontal diameter, vertical diameter and volume of C. oleifera seeds showed a rapid growth tendency from 235 days after pollination (DAP) to 258 DAP but had a slight increase at seed maturity. During seed development, the expression of genes related to cell proliferation and expansion differ greatly. Auxin plays an important role in C. oleifera seeds; YUC4 and IAA17 were significantly downregulated. Weighted gene co-expression network analysis screened 21 hub transcription factors for C. oleifera seed horizontal diameter, vertical diameter and volume. Among them, SPL4 was significantly decreased and associated with all these three traits, while ABI4 and YAB1 were significantly increased and associated with horizontal diameter of C. oleifera seeds. Additionally, KLU significantly decreased (2040-fold). Collectively, our data advances the knowledge of factors related to seed size and provides a theoretical basis for improving the yield of C. oleifera seeds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hormone analysis and candidate genes identification associated with seed size in Camellia oleifera

Song

et al. 2022

R. Soc. open sci.

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“…CsGL1 can directly bind to the promoters of CsACS genes and regulate their expression leading to ethylene accumulation changes and spine base development (Zhang et al, 2021). A number of ethylene-related genes were also identified in CsTTG1 overexpression lines and CsSBS1-RNAi lines (Guo et al, 2020;Fig. S9c).…”

Section: Cssbs1 Promotes Increases In Spine Base Size Through Interac...mentioning

confidence: 97%

“…Zhang et al (2021) found that CsGL1/CsTBH can directly bind to the promoters of cucumber 1-Aminocyclopropane-1-Carboxylate Synthase (CsACS) genes and regulate their expression leading to changes in ethylene accumulation and spine development. In previous studies, we have revealed that the WD-repeat protein CsTTG1 and MIXTA-like protein CsMYB6 regulated fruit spines initiation and further differentiation in cucumber (Chen et al, 2016;Yang et al, 2018Yang et al, , 2019Guo et al, 2020;Zhao et al, 2020). Recently, Wang et al (2021) identified a bHLH gene, CsHEC2, that regulated fruit spine density through mediating cytokinin biosynthesis and metabolism in cucumber.…”

Section: Introductionmentioning

confidence: 99%

A novel HD‐Zip I/C2H2‐ZFP/WD‐repeat complex regulates the size of spine base in cucumber

et al. 2022

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Fruit spine is an important trait in cucumber, affecting not only commercial quality, but also fruit smoothness, transportation and storage. Spine size is determined by a multi-cellular base. However, the molecular mechanism underlying the regulation of cucumber spine base remains largely unknown. Here, we report map-based cloning and characterization of a spine base size 1 (SBS1) gene, encoding a C2H2 zinc-finger transcription factor.Near-isogenic lines of cucumber were used to map, identify and quantify cucumber spine base size 1 (CsSBS1). Yeast-hybrid, bimolecular fluorescence complementation (BiFC), coimmunoprecipitation (Co-IP) and RNA-sequencing assays were used to explore the molecular mechanism of CsSBS1 in regulating spine base size development.CsSBS1 was specifically expressed in cucumber ovaries with particularly high expression in fruit spines. Overexpression of CsSBS1 resulted in large fruit spine base, while RNAinterference silencing of CsSBS1 inhibited the expansion of fruit spine base. Sequence analysis of natural cucumber accessions revealed that CsSBS1 was lost in small spine base accessions, resulting from a 4895 bp fragment deletion in CsSBS1 locus. CsSBS1 can form a trimeric complex with two positive regulators CsTTG1 and CsGL1 to regulate spine base development through ethylene signaling.A novel regulator network is proposed that the CsGL1/CsSBS1/CsTTG1 complex plays a significant role in regulating spine base formation and size, which offers a strategy for cucumber breeders to develop smooth fruit.

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“…In addition, as a comprehensive and accurate tool, high-throughput RNA sequencing (RNA-seq) provides an opportunity to quantify the transcriptome. Transcriptome profiling based on the RNA-seq is used to analyze gene expression patterns and the molecular mechanism in plant development, response to abiotic stresses and to map the response pathways [ 14 , 15 , 16 ]. A recent study comprised of both physiological and transcriptome analyses showed that glutathione (GSH) is a downstream signal of hydrogen sulfide (H 2 S)-induced tolerance to chilling stress in cucumber [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Unveiling Molecular Mechanisms of Nitric Oxide-Induced Low-Temperature Tolerance in Cucumber by Transcriptome Profiling

Kong

Bian

et al. 2022

IJMS

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Cucumber (Cucumis sativus L.) is one of the most popular cultivated vegetable crops but it is intrinsically sensitive to cold stress due to its thermophilic nature. To explore the molecular mechanism of plant response to low temperature (LT) and the mitigation effect of exogenous nitric oxide (NO) on LT stress in cucumber, transcriptome changes in cucumber leaves were compared. The results showed that LT stress regulated the transcript level of genes related to the cell cycle, photosynthesis, flavonoid accumulation, lignin synthesis, active gibberellin (GA), phenylalanine metabolism, phytohormone ethylene and salicylic acid (SA) signaling in cucumber seedlings. Exogenous NO improved the LT tolerance of cucumber as reflected by increased maximum photochemical efficiency (Fv/Fm) and decreased chilling damage index (CI), electrolyte leakage and malondialdehyde (MDA) content, and altered transcript levels of genes related to phenylalanine metabolism, lignin synthesis, plant hormone (SA and ethylene) signal transduction, and cell cycle. In addition, we found four differentially expressed transcription factors (MYB63, WRKY21, HD-ZIP, and b-ZIP) and their target genes such as the light-harvesting complex I chlorophyll a/b binding protein 1 gene (LHCA1), light-harvesting complex II chlorophyll a/b binding protein 1, 3, and 5 genes (LHCB1, LHCB3, and LHCB5), chalcone synthase gene (CSH), ethylene-insensitive protein 3 gene (EIN3), peroxidase, phenylalanine ammonia-lyase gene (PAL), DNA replication licensing factor gene (MCM5 and MCM6), gibberellin 3 beta-dioxygenase gene (GA3ox), and regulatory protein gene (NPRI), which are potentially associated with plant responses to NO and LT stress. Notably, HD-ZIP and b-ZIP specifically responded to exogenous NO under LT stress. Taken together, these results demonstrate that cucumber seedlings respond to LT stress and exogenous NO by modulating the transcription of some key transcription factors and their downstream genes, thereby regulating photosynthesis, lignin synthesis, plant hormone signal transduction, phenylalanine metabolism, cell cycle, and GA synthesis. Our study unveiled potential molecular mechanisms of plant response to LT stress and indicated the possibility of NO application in cucumber production under LT stress, particularly in winter and early spring.

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Transcriptome profiling reveals genes involved in spine development during CsTTG1-regulated pathway in cucumber (Cucumis sativus L.)

Cited by 16 publications

References 54 publications

Hormone analysis and candidate genes identification associated with seed size in Camellia oleifera

Hormone analysis and candidate genes identification associated with seed size in Camellia oleifera

A novel HD‐Zip I/C2H2‐ZFP/WD‐repeat complex regulates the size of spine base in cucumber

Unveiling Molecular Mechanisms of Nitric Oxide-Induced Low-Temperature Tolerance in Cucumber by Transcriptome Profiling

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