Transcriptome profiling reveals auxin suppressed anthocyanin biosynthesis in red-fleshed apple callus (Malus sieversii f. niedzwetzkyana)

Ji, Xiaohao; Zhang, Rui; Wang, Nan; Yang, Long; Chen, Xuesen

doi:10.1007/s11240-015-0843-y

Cited by 48 publications

(28 citation statements)

References 83 publications

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“…An alignment of the amino acid sequences of MYB12 and MYB22 showed that MYB12 contained the C‐terminal SG5 motif (V[I/x][R/x]T[K/x]Ax[R/K][L/C][T/S]) and MYB22 contained the C‐terminal SG7 motif ([K/R][R/x][R/K]xGRT[S/x][R/G]xx[M/x]K), suggesting that they had similar functions to the flavonoid regulators of other plants. However, some MYB TFs reported to be involved in flavonoid metabolism, such as MdMYB3 (Vimolmangkang et al ., ), MdMYB4 (Hellens et al ., ), MdMYB6 (Gao et al ., ) and MdMYB113 (Ji et al ., 2015b), did not contain SG5 or SG7 motif. Interestingly, Czemmel et al .…”

Section: Discussionmentioning

confidence: 97%

“…In red‐fleshed apple callus, some members of the MYB, bHLH and WD40 families involved in flavonoid synthesis were analyzed using RNA sequencing. The results showed that MYB10, MYB113, TT2 and TT8, which control anthocyanin and PA biosynthesis, were downregulated by high concentrations of naphthalene acetic acid and 2, 4‐dichlorophenoxyacetic acid (Ji et al ., 2015b). In this study, overexpression of MYB12 promoted the accumulation of PAs in apple callus, but not the accumulation of anthocyanins.…”

Section: Discussionmentioning

confidence: 99%

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MYB12 and MYB22 play essential roles in proanthocyanidin and flavonol synthesis in red‐fleshed apple (Malus sieversii f. niedzwetzkyana)

et al. 2017

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Flavonoids are major polyphenol compounds in plant secondary metabolism. Wild red-fleshed apples (Malus sieversii f. niedzwetzkyana) are an excellent resource because of their much high flavonoid content than cultivated apples. In this work, R6R6, R6R1 and R1R1 genotypes were identified in an F segregating population of M. sieversii f. niedzwetzkyana. Significant differences in flavonoid composition and content were detected among the three genotypes by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis. Furthermore, two putative flavonoid-related genes encoding R2R3-MYB transcription factors, designated MYB12 and MYB22, were cloned and characterized. The expression patterns of MYB12 and MYB22 directly correlated with those of leucoanthocyanidin reductase and flavonol synthase, respectively. Their roles in flavonoid biosynthesis were identified by overexpression in apple callus and ectopic expression in Arabidopsis. MYB12 expression in the Arabidopsis TT2 mutant complemented its proanthocyanidin-deficient phenotype. Likewise, MYB22 expression in an Arabidopsis triple mutant complemented its flavonol-deficient phenotype. MYB12 could interact with bHLH3 and bHLH33 and played an essential role in proanthocyanidin synthesis. MYB22 was found to activate flavonol pathways by combining directly with the flavonol synthase promoter. Our findings provide a valuable perspective on flavonoid synthesis and provide a basis for breeding elite functional apples with a high flavonoid content.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

MYB12 and MYB22 play essential roles in proanthocyanidin and flavonol synthesis in red‐fleshed apple (Malus sieversii f. niedzwetzkyana)

et al. 2017

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“…Plant hormones may be important factors regulating the light-induced anthocyanin accumulation. For example, auxin has been found to retard anthocyanin accumulation in apple and Arabidopsis [ 57 , 58 ]. In the present study, the transcript level of one auxin-associated gene ( Rsa1.0_02398.1_g00002.1 ) decreased (Additional file 2 : Table S2), implying that it may participate in anthocyanin biosynthesis under UV-A + HRW.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of radish sprouts hypocotyls reveals the regulatory role of hydrogen-rich water in anthocyanin biosynthesis under UV-A

Zhang

et al. 2018

BMC Plant Biol

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BackgroundHydrogen gas (H2) is the most abundant element in the universe, and has been reported to act as a novel beneficial gaseous molecule in plant adaptive responses. Radish sprouts are popular because they contain substantial amounts of antioxidants and health-promoting compounds, such as anthocyanin and glucosinolates. Although radish sprouts accumulated more anthocyanin under UV-A after treatment with hydrogen-rich water (HRW), the molecular mechanism responsible is still elusive. To explore these mechanisms, RNA-seq analysis was used.ResultsFour cDNA libraries from radish sprout hypocotyls were constructed, and a total of 14,564 differentially expressed genes (DEGs) were identified through pairwise comparisons. By Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, these unigenes were found to be implicated in light signal perception and transduction, starch and sucrose metabolism, photosynthesis, nitrogen metabolism and biosynthesis of secondary metabolites. The MYB-bHLH-WD40 complex accounted for the majority of the transcription factors found to be involved in anthocyanin biosynthesis, and levels of transcripts for this complex were in accordance with the anthocyanin concentrations observed. In addition, other transcription factors (such as NAC, bZIP and TCP) might participate in HRW-promoted anthocyanin biosynthesis. Furthermore, the signaling processes of plant hormones, MAPKs and Ca2+ might be involved in HRW-promoted anthocyanin biosynthesis under UV-A. The expression patterns of 16 selected genes were confirmed using qRT-PCR analysis.ConclusionsTaken together, the results of this study may expand our understanding of HRW-promoted anthocyanin accumulation under UV-A in radish sprouts.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1449-4) contains supplementary material, which is available to authorized users.

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“…Ji et al (2015) investigated the effects of auxin treatment on anthocyanin biosynthesis in calli derived from leaves of the red-fleshed apple M. niedzwetzkyana (type 1). They found that adding 1-naphthalene acetic acid and 2,4-D to the growth media inhibited anthocyanin production and downregulated the expression of anthocyanin biosynthetic and related TF genes in the calli.…”

Section: ) Other Factorsmentioning

confidence: 99%

Anthocyanin Biosynthesis in Apple Fruit

Honda

Moriya

2018

The Hortic J

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Anthocyanin accumulation is responsible for the red color of the skin and flesh of apple fruits (Malus × domestica Borkh.), and redder fruits are more marketable. Pigmentation in the skin of apple fruit varies among different cultivars and is influenced by environmental factors, including temperature conditions and the level of sunlight irradiation. Because warmer temperatures suppress anthocyanin synthesis in the skin of apple fruit, there are increasing concerns that global warming may be detrimental to fruit pigmentation. Recent molecular studies have revealed that the MdMYB1 gene, which encodes a transcription factor, plays a critical role in regulating anthocyanin synthesis in both the skin and flesh of apple fruits. A marker-assisted selection process has been developed to identify MdMYB1 genotypes and predict those fruits that will develop redder skin. These apples may be better adapted to a warmer global climate. The application of hormones can also increase the level of pigmentation in fruit skin, and plant growth regulators such as ethylene and jasmonate are commercially available. The mechanisms that regulate anthocyanin biosynthesis in the flesh of red-fleshed apple fruit appear to partially differ from those that function in the skin of red-skinned fruit. In the flesh of red-fleshed fruit, the pigment accumulates under dark conditions, whereas no anthocyanin is synthesized in the skin of bagged apple fruit. Conversely, in both red-skinned and red-fleshed apple fruits, warmer temperatures inhibit anthocyanin accumulation. Further studies on the regulation of anthocyanin synthesis in the flesh of red-fleshed apple fruit are necessary.

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Transcriptome profiling reveals auxin suppressed anthocyanin biosynthesis in red-fleshed apple callus (Malus sieversii f. niedzwetzkyana)

Cited by 48 publications

References 83 publications

MYB12 and MYB22 play essential roles in proanthocyanidin and flavonol synthesis in red‐fleshed apple (Malus sieversii f. niedzwetzkyana)

MYB12 and MYB22 play essential roles in proanthocyanidin and flavonol synthesis in red‐fleshed apple (Malus sieversii f. niedzwetzkyana)

Transcriptome analysis of radish sprouts hypocotyls reveals the regulatory role of hydrogen-rich water in anthocyanin biosynthesis under UV-A

Anthocyanin Biosynthesis in Apple Fruit

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