Transcriptome Profiling Revealed Basis for Growth Heterosis in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Zhu, Jinglin; Zou, Zhiying; Li, Dayu; Xiao, Wei; Yu, Jie; Chen, Binglin; Xue, Liyan; Yang, Hong

doi:10.3390/fishes7010043

Cited by 5 publications

(5 citation statements)

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“…In a previous study conducted by Xu et al [42], it was found that hybrid tilapia inherited all of the lactate dehydrogenase isoenzyme bands from parent Nile tilapia and only some of the lactate dehydrogenase isoenzyme bands from parent blue tilapia during electrophoresis. Similarly, Zhong et al [19] observed that GH genes exhibited asymmetric expression in hybrid tilapia, with the expression of the maternal sub-genome from Nile tilapia dominating in hybrid tilapia under both feeding and fasting conditions.…”

Section: Role Of Cis-acting Element Variants In P38 Allele-specific E...mentioning

confidence: 92%

“…These studies have made some progress and helped us to understand the mechanism of MAPK's role in fish. In our previous study, transcriptome analysis revealed that p38 showed non-additive expression in hybrid tilapia and played a key role in multiple enriched pathways such as the endocrine system, disease immunity, and the oxidative metabolism [25,26]. Hu et al [3] found that, in Nile tilapia and hybrid tilapia, S. agalactiae infection can activate the p38 MAPK pathway, and the rapid response and high level of protein expression of p38 MAPK may be the reason why hybrid tilapia have stronger bacterial resistance than Nile tilapia.…”

Section: Introductionmentioning

confidence: 99%

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Non-Additive and Asymmetric Allelic Expression of p38 mapk in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Liu,

Chen,

Zou

et al. 2024

Animals

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Hybridization is a widely used breeding technique in fish species that enhances desirable traits in cultured species through heterosis. However, the mechanism by which hybrids alter gene expression to form heterosis remains unclear. In this study, a group of hybrid tilapia was used to elucidate heterosis through interspecies crossing. Specifically, p38 was analyzed to describe the regulation of gene expression variation in hybrid tilapia. Transcripts from the Nile tilapia allele were found to be significantly higher than those from the blue tilapia allele in hybrid individuals, indicating that the expression of p38 was dominated by Nile tilapia sub-genomic alleles. The study also found a compensatory interaction of cis- and trans-acting elements of the Nile tilapia and blue tilapia sub-genomes, inducing a non-additive expression of p38 in hybrids. Eight specific SNPs were identified in the p38 promoter regions of Nile tilapia and blue tilapia, and were found to be promoter differences leading to differences in gene expression efficiencies between parental alleles using a dual-luciferase reporter system. This study provides insights into the non-additive expression patterns of key functional genes in fish hybrids related to growth and immunity response.

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Section: Role Of Cis-acting Element Variants In P38 Allele-specific E...mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Non-Additive and Asymmetric Allelic Expression of p38 mapk in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Liu,

Chen,

Zou

et al. 2024

Animals

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“…Ribosome biogenesis in eukaryotes includes the maturation of ribosomal RNA and assembly into ribosomal subunits [45]. Ribosome biogenesis determines the rate of protein synthesis and regulates the accumulation of cell mass or cell growth by controlling the major anabolic cellular processes [46]. The endoplasmic reticulum is involved in protein synthesis, folding, and processing and directs misfolded proteins to the proteasome for degradation [47].…”

Section: Effects Of High Stocking Density On Protein Metabolism-relat...mentioning

confidence: 99%

“…ACACA is involved in the first step of fatty acid synthesis and is also a key rate-limiting enzyme in this reaction, facilitating the catalytic conversion of acetyl CoA to malonyl CoA [71,72]. Subsequently, malonyl CoA undergoes successive condensation reactions with acetyl CoA in the presence of FASN, another key rate-limiting enzyme for fatty acid synthesis, to produce long-chain saturated fatty acids [46,71]. SCD1 and FADS2 exhibit fatty acid desaturation activity, wherein they can convert long-chain saturated fatty acids into their unsaturated counterparts.…”

Section: Effects Of High Stocking Density On Lipid Metabolism-related...mentioning

confidence: 99%

Transcriptome-Based Analysis of the Liver Response Mechanism of Black Porgy (Acanthopagrus schlegelii) to Stocking Density

Zhou

Jia

Meng

et al. 2023

Fishes

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Long-term high stocking density often brings negative effects such as decreased body weight, decreased immunity, and increased mortality to cultured fish, while the effects of short-term stocking are relatively less studied. In this experiment, we characterized spatial and temporal gene expression in black porgy (Acanthopagrus schlegelii) liver by establishing two stocking density groups—high (H: 26.5 kg/m3) and low (L: 2.95 kg/m3)—and conducting transcriptome sequencing before collecting liver samples at two time points: 6 h (AL and AH) and 60 d (CL and CH) into the culture trial. There were 648 and 550 differentially expressed genes (DEGs) in the AL-vs-AH and CL-vs-CH groups, respectively. The DEGs in the AL-vs-AH group were significantly enriched in steroid biosynthesis, terpenoid backbone biosynthesis, the PPAR signaling pathway, proteasome, aminoacyl-tRNA biosynthesis, and ribosome biogenesis in eukaryotes. The DEGs in the CL-vs-CH group were more significantly enriched in lipid metabolism-related pathways, such as the PPAR signaling pathway, fatty acid metabolism, and cholesterol metabolism. In addition, immune-related pathways such as the phagosome and complement and coagulation cascades were also enriched in the CL-vs-CH group. The expression changes concerning the DEGs in each group were further analyzed. The DEGs related to steroid synthesis, protein synthesis, and the degradation pathways were down-regulated, but immune-related genes were generally up-regulated in the AH group. The DEGs related to lipid synthesis were significantly up-regulated in the CH group, but the DEGs related to lipid consumption and utilization were down-regulated. The expression of immune-related DEGs was also negatively affected in the CH group. These results suggest that acute and chronic crowding stress affects lipid metabolism and immune regulation in the liver of black porgy. Acute stress particularly affected hepatic protein metabolism, while chronic stress showed more effects on hepatic lipid metabolism. The results of this study provide a theoretical basis for understanding the effects of high stocking densities upon black porgy and may also provide a reference for crowding stress regulation studies in other fish species.

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“…The Nile tilapia (♀) × blue tilapia (♂) hybrid is one of the most popular farmed tilapia varieties in China, due to its fast growth performance and strong resistance to low temperature ( 28 ). This tilapia variety also showed higher appetite ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

Nonadditive and allele-specific expression of ghrelin in hybrid tilapia

Zhong,

Ren,

Lou

et al. 2023

Front. Endocrinol.

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BackgroundInterspecies hybridization is an important breeding method to generate fishes with heterosis in aquaculture. Using this method, hybrid Nile tilapia (Oreochromis niloticus, ♀) × blue tilapia (Oreochromis aureus, ♂) has been produced and widely farmed due to its growth and appetite superiorities. However, the genetic mechanism of these advanced traits is still not well understood. Ghrelin is a crucial gene that regulates growth and appetite in fishes. In the present study, we focused on the expression characteristics and its regulation of ghrelin in the hybrid.ResultsThe tissue distribution analysis showed that ghrelin was predominantly expressed in the stomach in the hybrid. Ghrelin was more highly expressed in the stomach in the hybrid and Nile tilapia, compared to blue tilapia, showing a nonadditive pattern. Two single-nucleotide polymorphism (SNP) sites were identified including T/C and C/G from the second exon in the ghrelin gene from Nile tilapia and blue tilapia. By pyrosequencing based on the SNP sites, the allele-specific expression (ASE) of ghrelin in the hybrid was assayed. The result indicated that ghrelin in the hybrid showed higher maternal allelic transcript ratios. Fasting significantly increased ghrelin overall expression at 4, 8, 12, 24, and 48 h. In addition, higher maternal allelic transcript ratios were not changed in the fasting hybrids at 48 h. The cis and trans effects were determined by evaluating the overall expression and ASE values in the hybrid. The expression of ghrelin was mediated by compensating cis and trans effects in hybrid.ConclusionIn summary, the present lines of evidence showed the nonadditive expression of ghrelin in the hybrid tilapia and its regulation by subgenomes, offering new insight into gene expression characteristics in hybrids.

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Transcriptome Profiling Revealed Basis for Growth Heterosis in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Cited by 5 publications

References 50 publications

Non-Additive and Asymmetric Allelic Expression of p38 mapk in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Non-Additive and Asymmetric Allelic Expression of p38 mapk in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Transcriptome-Based Analysis of the Liver Response Mechanism of Black Porgy (Acanthopagrus schlegelii) to Stocking Density

Nonadditive and allele-specific expression of ghrelin in hybrid tilapia

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