Transcriptome profiling of the newborn mouse lung after hypoxia and reoxygenation: hyperoxic reoxygenation affects mTOR signaling pathway, DNA repair, and JNK-pathway regulation

Wollen, Embjørg J.; Sejersted, Yngve; Wright, Marianne; Bik-Multanowski, Mirosław; Madetko‐Talowska, Anna; Günther, Clara‐Cecilie; Nygård, Ståle; Kwinta, Przemko; Pietrzyk, Jacek J; Saugstad, Ola Didrik

doi:10.1038/pr.2013.140

Cited by 21 publications

(45 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our hypoxia model resulted in physiological stress, demonstrated with significant metabolic acidosis (17) and cardiac and respiratory changes in line with the definition of intrapartum fetal asphyxia (18). A significant increase of microglial cells was observed after hypoxia-reoxygenation both in striatal tissue and in the hippocampal CA1, but with no differences between the reoxygenation regimes.…”

Section: Discussionmentioning

confidence: 79%

“…The microarray data were preprocessed using the R/Bioconductor package Robust Multiarray Average (RMA) (Fred Hutchinson Cancer Research Center, Seattle, WA) as previously described (17). In RMA, raw intensity values are background corrected, log2 transformed and then quantile normalized, before an additive linear model is applied to the normalized data.…”

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

“…Primers were designed for each transcript (Hmox1, Stat3, Osmr, Igf1r, Mt2, Cox6a, and Hdac5) using Primer Express 3.0 Software (Applied Biosystems, Grand Island, NY) (see Supplementary Table S7 online). Total mRNA was extracted, reverse transcribed, amplified, and analyzed as described previously (17). Briefly, RT-PCR was performed with 50 ng of cDNA and 400 nmol/l primers for the target genes and reference gene PPIA (peptidylprolyl isomerase A).…”

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

“…Microarray analysis was performed according to the manufacturer's protocol (GeneChip Whole Transcript sense Target Labeling Assay Manual, version 4), and described previously (17). For further statistical analysis, **.cel files generated by GeneChip Operating Software (Affymetrix) were used (see Supplementary Methods online).…”

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

“…The olfactory bulb and the cerebellum were removed, and the forebrain was subsequently preserved in RNA later stabilization reagent at −20 °C (Qiagen, Hilden, Germany). RNA extraction was performed as described previously (17).…”

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

See 4 more Smart Citations

Transcriptome profiling of the newborn mouse brain after hypoxia–reoxygenation: hyperoxic reoxygenation induces inflammatory and energy failure responsive genes

et al. 2013

Self Cite

View full text Add to dashboard Cite

Background: Supplemental oxygen used during resuscitation can be detrimental to the newborn brain. The aim was to determine how different oxygen therapies affect gene transcription in a hypoxia-reoxygenation model. Methods: C57BL/6 mice (n = 56), postnatal day 7, were randomized either to 120 min of hypoxia 8% O 2 followed by 30 min of reoxygenation with 21, 40, 60, or 100% O 2 , or to normoxia followed by 30 min of 21 or 100% O 2 . Affymetrix 750k expression array was applied with RT-PCR used for validation. Histopathology and immunohistochemistry 3 d after hypoxiareoxygenation compared groups reoxygenated with 21 or 100% O 2 with normoxic controls (n = 22). results: In total, ~81% of the gene expression changes were altered in response to reoxygenation with 60 or 100% O 2 and constituted many inflammatory-responsive genes (i.e., C5ar2, Stat3, and Ccl12). Oxidative phosphorylation was downregulated after 60 or 100% O 2 . Iba1 + cells were significantly increased in the striatum and hippocampal CA1 after both 21 and 100% O 2 . conclusion: In the present model, hypoxia-reoxygenation induces microglial accumulation in subregions of the brain. The transcriptional changes dominating after applying hyperoxic reoxygenation regimes include upregulating genes related to inflammatory responses and suppressing the oxidative phosphorylation pathway.

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

Section: Gene Expression In the Newborn Brainmentioning

confidence: 99%

See 3 more Smart Citations