Transcriptome profiling of pluripotent pig embryonic stem cells originating from uni- and biparental embryos

Abstract

Objectives: Pig pluripotent stem cells have tremendous potential because the pig is a valuable animal as both an agricultural resource and as a preclinical model of human therapy. To date, a lack of understanding of pig pluripotency has inhibited the derivation of embryonic stem cells (ESCs) and transgene-free induced pluripotent stem cells. Therefore, there has been no accessible or reliable transcriptome data for researching the genuine pig pluripotency network. Our previous study isolated authentic pig ESCs, which had teratoma-forming and direct differentiation ability, that were derived by activating the FGF2, ACTIVIN A, and WNT pathways. Here, we aimed to provide detailed information on transcriptome data of the newly derived pig ESCs and perform a comparative analysis with pig preimplantation embryo transcriptomes in a public database. Data description: The transcriptome data of ESCs derived from in vitro fertilized and parthenogenetic embryos were generated by HiSeq 2500. Then, differentially expressed genes (DEGs) from each sample were compared with fibroblasts, and gene expression profiling was carried out for comparative analysis. Our data, as the first transcriptome dataset for genuine pig pluripotent cells, could be a general reference for explaining the molecular mechanism of species-specific pluripotency and improving understanding of the embryo development of domestic animals.

Melatonin promotes the proliferation of primordial germ cell‐like cells derived from porcine skin‐derived stem cells: A mechanistic analysis

“…In the reproductive area, the generation of PGCLCs in vitro would assist in understanding the mechanisms involved in cellular differentiation and the epigenetic changes. PGCLCs, formed from nonembryonic sources, would be a suitable alternative for studies which are difficult to perform in humans 13,18–20 . In addition, due to the advantages of easy obtainment and abundant material sources, the differentiation of SDSCS into GCs has received extensive attention 17,21–23 .…”

“…PGCLCs, formed from nonembryonic sources, would be a suitable alternative for studies which are difficult to perform in humans. 13,[18][19][20] In addition, due to the advantages of easy obtainment and abundant material sources, the differentiation of SDSCS into GCs has received extensive attention. 17,[21][22][23] As early as 2006, Dyce et al 24 demonstrated that SDSCs isolated from porcine fetal skin can be induced to PGCLCs in vitro, but the efficiency was very low.…”

The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances

“…This medium not only allowed for the expansion of SOX2-expressing cells from the ICM outgrowths, but also enabled the derivation of stable pESC lines from both IVF- and parthenogenetically-derived embryos ( Choi et al, 2019 ). pESC lines were stably maintained for more than 1 year while maintaining stemness and a normal karyotype ( Choi, Lee, Oh, Kim, Lee, Woo, et al, 2020 ). Interestingly, RNA-seq analysis showed that pESCs are transcriptionally closer to an epiblast-like state than to the ICM state ( Secher et al, 2017 ; Choi, Lee, Oh, Kim, Lee, Kim, et al, 2020 ).…”

“…pESC lines were stably maintained for more than 1 year while maintaining stemness and a normal karyotype ( Choi, Lee, Oh, Kim, Lee, Woo, et al, 2020 ). Interestingly, RNA-seq analysis showed that pESCs are transcriptionally closer to an epiblast-like state than to the ICM state ( Secher et al, 2017 ; Choi, Lee, Oh, Kim, Lee, Kim, et al, 2020 ).…”

Identification of key modules and hub genes involved in regulating the feather follicle development of Wannan chickens using WGCNA

“…The Wannan Chickens is a local breed of chicken native to China, with a distribution that extends along the river in southern and eastern Anhui. In recent years, transcriptome analysis has been employed extensively applied to study of various species and traits ( Choi, et al, 2020 ; Cassar-Malek, et al, 2022 ; Jin, et al, 2022 ), Furthermore, it has been successfully employed in the study of poultry traits ( Ren, et al, 2021 ; Pham, et al, 2022 ; Sun, et al, 2023 ). Weighted gene co-expression network analysis ( WGCNA ) has also been widely used to identify key modules of traits and important genes in a multitude of species ( Fan, et al, 2023 ; Yu, et al, 2023 ).…”