Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

Schemberger, Michelle Orane; Stroka, Marília Aparecida; Reis, Lúcia Margarete dos; Los, Kamila Karoline de Souza; Araujo, Gillize Aparecida Telles de; Sfeir, Michelle Zibetti Tadra; Galvão, Carolina Weigert; Etto, Rafael Mazer; Baptistão, Amanda Regina Godoy; Ayub, Ricardo Antônio

doi:10.1186/s12864-020-6667-0

Cited by 18 publications

(10 citation statements)

References 65 publications

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“…In the phenotypic characterization of climacteric and non-climacteric cultivars the colorimetric results showed similar behavior of hue angle for peel and pulp in 'Yellow' melon grown in Brazil and Canada as well as to those found by Schemberger et al (Schemberger et al 2020). For 'Gaúcho' melons, a few differences between melons grown in Brazil and Canada were observed, being the hue angle of the melon peel of 15 and 25 DAP melons and in the melon pulp of 35 and 45 DAP melons.…”

Section: Discussionsupporting

confidence: 80%

“…Expression of CmLOX9 decreased from 35 DAP to 45 DAP in 'Yellow' melons as the fruit ripened. Similar results were found in RNA-Seq analysis of 'Yellow' melon, where expression of the CmLOX9 gene was higher in 10 DAP than 40 DAP melons (Schemberger et al 2020). In 'Gaúcho' melons, the expression of this gene remained relatively constant at 35 DAP and 45 DAP and was lower than expression in 'Yellow' melons at both stages.…”

Section: Lipid Metabolismsupporting

confidence: 82%

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Comparative Analyses of Volatile Metabolites Associated With Gene Expression From Non-climacteric and Climacteric Melon.

Los

Schemberger

Reis

et al. 2021

Preprint

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Melon (Cucumis melo L.) is an important species in the cucurbits family with a large economic importance in the world. Two melon cultivars commercially important in Brazil are the cultivars ‘Yellow’ and ‘Gaúcho’. In addition to the economic importance, these two cultivars display phenotypic differences in aroma, a major trait determining fruit quality. Volatile organic compounds (VOC) impart the different aroma found in these fruit and its biosynthesis is associated with fatty acid and amino acid metabolism. Using SPME-GC–MS and RT-qPCR techniques, volatile production and expression of seven genes (CmLOX9, CmLOX18, CmBCAT1, CmArAT1, CmPDC1, CmADH1 and CmAAT1) were determined during maturation and ripening. The climacteric melon ‘Gaúcho’ had a greater number and higher concentration of volatiles than that in the non-climacteric ‘Yellow’ melon. 2-Methylallyl acetate, 4-amino-1-butanol, 2-methylbutanol and ethyl 2-methylpropanoate were found in high concentrations in ripe climacteric ‘Gaúcho’ melons and were major contributors to its strong fruity aroma, but high concentrations of these volatiles were not found in non-climacteric ‘Yellow’ melons. The lipid pathway played a strong role in determining aroma composition in non-climacteric ‘Yellow’ melons. Most volatiles decreased during maturation and ripening explaining the non-aromatic characteristic of this cultivar. In climacteric ‘Gaúcho’ melons, the amino acid pathway was the main one related to the biosynthesis of esters, which contribute to the aroma of this cultivar. Volatile products of the branched chain amino acid pathway correlated with CmADH1 and CmAAT1 expression demonstrating their role in volatile synthesis of this climacteric melon cultivar. In addition, CmPDC1 contributes to the formation of aldehydes at the beginning of this pathway.

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Section: Discussionsupporting

confidence: 80%

Section: Lipid Metabolismsupporting

confidence: 82%

Comparative Analyses of Volatile Metabolites Associated With Gene Expression From Non-climacteric and Climacteric Melon.

Los

Schemberger

Reis

et al. 2021

Preprint

Self Cite

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“…S10 ) of phloem unloading in the expanding stage versus the premature stage in sweet melons. High anabolism and catabolism of sugar are observed at the beginning of fruit development, which are the metabolic processes required for carbon skeleton construction and energy supply in plants [ 50 ]. During the expanding stage of melon, NAG2, NAG3, AAG1, and AAG2 initially hydrolyze RFOs and then NIN1 and NIN4 hydrolyze sucrose into hexoses.…”

Section: Discussionmentioning

confidence: 99%

Phloem unloading in cultivated melon fruits follows an apoplasmic pathway during enlargement and ripening

Zhou

Wen

et al. 2023

Horticulture Research

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Melon (Cucumis melo L.) has a long history of cultivation worldwide. During cultivation, domestication, and selection breeding, the sugar content of mature melon fruits has been significantly increased. Compared with un-sweet melon and wild melon, rapid sucrose accumulation can occur in the middle and late stages of sweet melon fruit development. During the evolution and development of melon fruit, the phloem unloading pathway has not been identified and analyzed. In this study, the phloem unloading pathway and the function of related sugar transporters in cultivated and wild melon fruits were analyzed by CFDA and esculin tracing, cytological pathway observation, qRT-PCR, and gene function analysis, etc. Results show that phloem unloading pathway of wild melon fruit is largely symplastic, whereas phloem unloading pathway of cultivated melon fruit shifts from symplastic to apoplasmic during development. According to fruit grafting experiment, fruit sink accumulates sugars independently. The results of the correlation analysis showed that the expression amounts of several sucrose transporter genes were positively correlated with the sucrose content of melon fruit. Furthermore, CmSWEET10 was proved to be a sucrose transporter located on the plasma membrane of the phloem and highly expressed in the premature stage of sweet melon fruits, which means it may be involved in phloem apoplast unloading and sucrose accumulation in sweet melon fruits. Finally, we summarized a functional model of related enzymes and sugar transporters involved in the apoplast unloading of sweet melon fruits during enlargement and sucrose accumulation.

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“…Phenylalanine ammonia-lyase is one of the important enzymes associated with fruit ripening in mango ( Palafox-Carlos et al, 2014 ). Galactokinase was reported to play important role in cell wall stability in the ripening process of yellow melon ( Schemberger et al, 2020 ). It was further established that circRNAs were involved in determining the shelf-life of cucumber fruits in the natural variant, DC-48 with exceptionally high shelf-life and minimum post-harvest degradation through the regulation of the important genes associated with fruit ripening and cell wall stability.…”

Section: Discussionmentioning

confidence: 99%

Genome wide identification of lncRNAs and circRNAs having regulatory role in fruit shelf life in health crop cucumber (Cucumis sativus L.)

et al. 2022

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Cucumber is an extremely perishable vegetable; however, under room conditions, the fruits become unfit for consumption 2–3 days after harvesting. One natural variant, DC-48 with an extended shelf-life was identified, fruits of which can be stored up to 10–15 days under room temperature. The genes involved in this economically important trait are regulated by non-coding RNAs. The study aims to identify the long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) by taking two contrasting genotypes, DC-48 and DC-83, at two different fruit developmental stages. The upper epidermis of the fruits was collected at 5 days and 10 days after pollination (DAP) for high throughput RNA sequencing. The differential expression analysis was performed to identify differentially expressed (DE) lncRNAs and circRNAs along with the network analysis of lncRNA, miRNA, circRNA, and mRNA interactions. A total of 97 DElncRNAs were identified where 18 were common under both the developmental stages (8 down regulated and 10 upregulated). Based on the back-spliced reads, 238 circRNAs were found to be distributed uniformly throughout the cucumber genomes with the highest numbers (71) in chromosome 4. The majority of the circRNAs (49%) were exonic in origin followed by inter-genic (47%) and intronic (4%) origin. The genes related to fruit firmness, namely, polygalacturonase, expansin, pectate lyase, and xyloglucan glycosyltransferase were present in the target sites and co-localized networks indicating the role of the lncRNA and circRNAs in their regulation. Genes related to fruit ripening, namely, trehalose-6-phosphate synthase, squamosa promoter binding protein, WRKY domain transcription factors, MADS box proteins, abscisic stress ripening inhibitors, and different classes of heat shock proteins (HSPs) were also found to be regulated by the identified lncRNA and circRNAs. Besides, ethylene biosynthesis and chlorophyll metabolisms were also found to be regulated by DElncRNAs and circRNAs. A total of 17 transcripts were also successfully validated through RT PCR data. These results would help the breeders to identify the complex molecular network and regulatory role of the lncRNAs and circRNAs in determining the shelf-life of cucumbers.

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Transcriptome profiling of non-climacteric ‘yellow’ melon during ripening: insights on sugar metabolism

Cited by 18 publications

References 65 publications

Comparative Analyses of Volatile Metabolites Associated With Gene Expression From Non-climacteric and Climacteric Melon.

Comparative Analyses of Volatile Metabolites Associated With Gene Expression From Non-climacteric and Climacteric Melon.

Phloem unloading in cultivated melon fruits follows an apoplasmic pathway during enlargement and ripening

Genome wide identification of lncRNAs and circRNAs having regulatory role in fruit shelf life in health crop cucumber (Cucumis sativus L.)

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