Transcriptome profiling of Microbacterium resistens MZT7 reveals mechanisms of 17β-estradiol response and biotransformation

Hao, Peng; Lv, Zongshuo; Wu, Sicheng; Zhang, Xiqing; Gou, Changlong; Wang, Lixia; Zhu, Yanbin; Basang, Wangdui; Gao, Yunhang

doi:10.1016/j.envres.2022.114963

Cited by 5 publications

(5 citation statements)

References 45 publications

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“…However, reports on E2 degradation by SDRs are still limited, especially studies on E2 removal from wastewater. In a prior investigation, it was discovered that E2 induction increase the expression of 17-HSD-0095 in the strain MZT7, and heterologously expressed recombinant cells had the capacity to degrade E2 [ 34 ]. In this study, the 17β-HSD-0095 protein was purified and its enzymatic properties were investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The E. coli BL21 (DE3) cells containing recombinant plasmid (pET-0095) were already preserved in our lab [ 34 ]. The strains were cultured in Luria–Bertani (LB) medium.…”

Section: Methodsmentioning

confidence: 99%

“…Degradation and relative enzyme activity experiments were performed using Mineral Salt Medium (MSM). LB and MSM were prepared as previously described [ 34 ]. E2 (> 99% purity) was purchased from Solarbio Scientific Co., Ltd. (Beijing, China), nicotinamide adenine dinucleotide trihydrate (NAD + ) from Sangon Biotech Co., Ltd. (Shanghai, China) and acetonitrile and methanol of HPLC quality were supplied by Thermo Fisher Scientific Co., Ltd (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…The extraction and HPLC (Shimadzu LC-2030 plus, Japan) detection of E2 in the degradation system was performed as previously described with minor modifications [ 34 ]. Briefly, an equal volume of methanol was added to the degradation system, the mixture was dissolved by ultrasonication and centrifuged at 10,000 rpm for 5 min, and the supernatant was filtered through a 0.22 μm filter and detected by HPLC/UV for estrogen concentration.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown that strain MZT7 can degrade E2 to E1 [ 22 ]. In another recent study, a heterologously expressed short-chain dehydrogenase gene (MZT7_GM000095, locus_tag = K8F61_00480) was confirmed to transform E2 to E1 [ 34 ]. In this work, an E2-degrading enzyme was expressed from strain MZT7 and characterized by biochemical characterization experiments.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

Hao

Pan

et al. 2023

Microb Cell Fact

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Background 17β-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies having great potential for removal E2 residues from the environment. However, limited literature is available on the removal of E2 from wastewater using short-chain dehydrogenase. Results In this study, 17β-estradiol degrading enzyme (17β-HSD-0095) was expressed and purified from Microbacterium sp. MZT7. The optimal pH and temperature for reaction was 7 and 40 °C, respectively. Molecular docking studies have shown that the ARG215 residue form a hydrogen bond with oxygen atom of the substrate E2. Likewise, the point mutation results have revealed that the ARG215 residue play an important role in the E2 degradation by 17β-HSD-0095. In addition, 17β-HSD-0095 could remediate E2 contamination in synthetic livestock wastewater. Conclusions These findings offer some fresh perspectives on the molecular process of E2 degradation and the creation of enzyme preparations that can degrade E2. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

“…The E. coli BL21 (DE3) cells containing recombinant plasmid (pET-0095) were already preserved in our lab [ 34 ]. The strains were cultured in Luria–Bertani (LB) medium.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

Hao

Pan

et al. 2023

Microb Cell Fact

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Biodegradation of 17β‐estradiol by drug‐resistant Stenotrophomonas maltophilia MN08 and Pseudomonas aeruginosa KL10 isolated from the sediment sample

Farraj,

Gawwad,

Elshikh

et al. 2024

Environmental Quality Mgmt

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Abstract17 β‐Estradiol is produced by animals, and humans and excreted through excreta, and has endocrine‐disrupting activity. Bacterial degradation is one of the suitable methods used for the removal of 17 β‐estradiol (E2) from the natural environment. In this study, E2‐degrading bacteria were screened from the enriched sediment sample from the wastewater environment. Two bacterial strains of E2‐degrading bacteria (Stenotrophomonas maltophilia MN08 and Pseudomonas aeruginosa KL10) were determined and >70% of E2‐degradation was achieved. The strain MN08 could rapidly remove approximately 86.5% ± 1.3% of E2 within 7 days of treatment. In addition, the strain KL10 removed 79.5% ± 1% of E2 within the same period. Optimized culture temperature and pH of the medium could improve E2‐degradation. The results demonstrated that the strain MN08 exhibited maximum E2‐degradation activity at 35°C, and pH 8.0. The optimal pH for strain KL10 to degrade E2 ranged from 7.0 to 9.0. The E2‐degradation level for the mixture culture systems can exhibit synergistic E2‐degradation activity. The degradation of the MN08 and KL10 individual strains was 87.3% ± 1.2% and 81.4% ± 1.5%, respectively. The E2‐degradation potential for the mixed culture system was 91.3% ± 2.9% under optimized conditions in which 100 mg/L of E2 served as the sole source of carbon and energy. The present finding demonstrates that the co‐culture of S. maltophilia MN08 and P. aeruginosa KL10 may provide a promising bio‐treatment strategy for the removal of steroids from wastewater.

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Microbial bioremediation—A sustainable technique of pollution abatement

Hashem,

Dawoud,

Almutairi

et al. 2024

Microbiome-Assisted Bioremediation

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Transcriptome profiling of Microbacterium resistens MZT7 reveals mechanisms of 17β-estradiol response and biotransformation

Cited by 5 publications

References 45 publications

Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

Biodegradation of 17β‐estradiol by drug‐resistant Stenotrophomonas maltophilia MN08 and Pseudomonas aeruginosa KL10 isolated from the sediment sample

Microbial bioremediation—A sustainable technique of pollution abatement

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