Transcriptome profiling of lung schistosomula,in vitro cultured schistosomula and adult Schistosoma japonicum

Chai, M.; McManus, Donald P.; McInnes, Russell; Moertel, Luke; Tran, Minh; Loukas, Alex; Jones, Malcolm K.; Gobert, Geoffrey N.

doi:10.1007/s00018-005-5578-1

Cited by 71 publications

(74 citation statements)

References 42 publications

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“…In vitro cultured and ex vivo lung-stage schistosomula did not differ greatly in respect to IF reactivity or intensity with anti-SG3PDH sera, suggesting that SG3PDH expression was not sensitive to the impossibility of reproducing the host environmental factors in vitro (Chai et al 2006). We have used antibodies to peptides based on the amino acid sequence of SG3PDH, and not to the recombinant molecule, to ascertain the specificity of the antigenantibody reaction and avoid possible artifacts resulting from the binding of antibodies directed to traces of bacterial proteins.…”

Section: Discussionmentioning

confidence: 95%

Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase is a lung-stage schistosomula surface membrane antigen

Tallima¹,

Ridi²

2008

FOLIA PARASIT

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Abstract. We have previously reported that Schistosoma mansoni larvae emerging from host lung at pH 7.5-7.8 and then fixed with diluted formaldehyde (HCHO) readily bind radiation-attenuated cercariae (RA) vaccine serum antibodies, as assessed by indirect membrane immunofluorescence (IF). Here we show that S. mansoni schistosomula emerging from lung pieces under 5% CO 2 (pH ≤7.3) readily bind RA vaccine serum antibodies, provided they have been incubated for 12 h at pH 7.5-7.8 in foetal calf serum-free RPMI medium, and fixed with diluted HCHO. Ex vivo larvae exposed during incubation to GW4869, a specific inhibitor of tegument-bound, neutral sphingomyelinase (nSMase) displayed significantly diminished binding of RA vaccine serum antibodies, thus suggesting that nSMase activity at pH ≥7.5 leads to exposure of lung-stage larvae surface membrane antigens to specific antibody detection. More importantly, ex vivo larvae readily bound antibodies directed to dipeptidic multiple antigen peptide constructs, based on S. mansoni-specific sequences in S. mansoni glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). Lung-stage schistosomula IF reactivity was diminished following antiserum absorption with recombinant SG3PDH. The data together indicate that intact ex vivo, as well as, 5-day-old in vitro-grown larvae express SG3PDH on their surface membrane. The findings are discussed in relation to the importance of surface membrane proteins as candidate vaccine antigens.

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Section: Discussionmentioning

confidence: 95%

Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase is a lung-stage schistosomula surface membrane antigen

Tallima¹,

Ridi²

2008

FOLIA PARASIT

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“…The use of DNA microarrays has been widely applied to schistosomes and is considered the method-of-choice for "global" or genome-wide evaluation of gene expression among the mammalian host stages (Fitzpatrick et al, 2004;Fitzpatrick et al, 2005;Chai et al, 2006;DeMarco et al, 2006;Gobert et al, 2006;Moertel et al, 2006) and those involved in larval development within the snail host (Vermeire et al, 2006). However, a major drawback of microarrays is their dependence upon pre-selection of probes.…”

Section: Introductionmentioning

confidence: 99%

Profiling Schistosoma mansoni development using serial analysis of gene expression (SAGE)

Williams

Sayed

Bernier

et al. 2007

Experimental Parasitology

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Despite the widespread use of chemotherapy and other control strategies over the past 50 years, transmission rates for schistosomiasis have changed little. Regardless of the approach used, future control efforts will require a more complete understanding of fundamental parasite biology. Schistosomes undergo complex development involving an alteration of parasite generations within a mammalian and freshwater molluscan host in the completion of its lifecycle. Little is known about factors controlling schistosome development, but understanding these processes may facilitate the discovery of new control methods. Therefore, our goal in this study is to determine global developmentally-regulated and stage-specific gene expression in Schistosoma mansoni using Serial Analysis of Gene Expression (SAGE). We present a preliminary analysis of genes expressed during development and sexual differentiation in the mammalian host and during early larval development in the snail host. A number of novel, differentially expressed genes have been identified, both within and between the different developmental stages found in the mammalian and snail hosts.

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“…dergo a number of morphological changes and physiological adaptations in their mammalian definitive hosts, and finally develop into mature adult worms. Several research groups have focused on the schistosomulum transcriptome or proteome with the purpose of identifying key molecules that are differentially expressed at this stage and which may therefore be essential for the survival and development of schistosome (8,(22)(23)(24)(25). These studies have provided valuable information for screening vaccine candidates and new drug targets.…”

Section: Proteomics Analysis Of Schistosoma Japonicummentioning

confidence: 99%

Proteomic Analysis of Schistosoma japonicum Schistosomulum Proteins that are Differentially Expressed Among Hosts Differing in Their Susceptibility to the Infection

Hong

Peng

Jiang

et al. 2011

Molecular & Cellular Proteomics

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Schistosomiasis is a tropical, parasitic disease affecting humans and several animal species. The aim of this study was to identify proteins involved in the growth and survival of the parasitic forms inside a host. Schistosomula of Schistosoma japonicum were isolated from three different hosts: the susceptible BALB/c mice; the Wistar rats, which have a considerably lower susceptibility; and the resistant reed vole, Microtus fortis. Soluble proteins of the schistosomula collected from the above three hosts 10 days postinfection were subjected to two-dimensional difference gel electrophoresis. Comparative proteomic analyses revealed that 39, 21, and 25 protein spots were significantly differentially expressed between schistosomula from mice and rats, mice and reed voles, or rats and reed voles, respectively (ANCOVA, p < 0.05). Further, the protein spots were identified by liquid chromatographytandem MS. Bioinformatics analysis showed that the differentially expressed proteins were essentially those involved in the metabolism of proteins, ribonucleotides, or carbohydrates, or in stress response or cellular movement. This study represents the first attempt at profiling S.

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Transcriptome profiling of lung schistosomula,in vitro cultured schistosomula and adult Schistosoma japonicum

Cited by 71 publications

References 42 publications

Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase is a lung-stage schistosomula surface membrane antigen

Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase is a lung-stage schistosomula surface membrane antigen

Profiling Schistosoma mansoni development using serial analysis of gene expression (SAGE)

Proteomic Analysis of Schistosoma japonicum Schistosomulum Proteins that are Differentially Expressed Among Hosts Differing in Their Susceptibility to the Infection

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