Transcriptome Profiling of Cucumber (Cucumis sativus L.) Early Response to Pseudomonas syringae pv. lachrymans

Słomnicka, Renata; Olczak-Woltman, Helena; Sobczak, Mirosław; Bartoszewski, Grzegorz

doi:10.3390/ijms22084192

Cited by 9 publications

(6 citation statements)

References 67 publications

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“…Both the cytological and transcriptomic responses of cucumber to Pseudomonas syringae pv. lachrymans (Psl) were characterized to elucidate the mechanism underlying cucumber resistance to bacterial angular leaf spot disease [28].…”

Section: Transcriptome Research Of Cucumbermentioning

confidence: 99%

Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement

Han

Ma²,

Zhang

et al. 2022

Plants

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Cucumbers are rich in vitamins and minerals. The cucumber has recently become one of China’s main vegetable crops. More specifically, the adjustment of the Chinese agricultural industry’s structure and rapid economic development have resulted in increases in the planting area allocated to Chinese cucumber varieties and in the number of Chinese cucumber varieties. After complete sequencing of the “Chinese long” genome, the transcriptome, proteome, and metabolome were obtained. Cucumber has a small genome and short growing cycle, and these traits are conducive to the application of molecular breeding techniques for improving fruit quality. Here, we review the developments and applications of molecular markers and genetic maps for cucumber breeding and introduce the functions of gene families from the perspective of genomics, including fruit development and quality, hormone response, resistance to abiotic stress, epitomizing the development of other omics, and relationships among functions.

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Section: Transcriptome Research Of Cucumbermentioning

confidence: 99%

Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement

Han

Ma²,

Zhang

et al. 2022

Plants

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“…For instance, cucumber bacterial angular leaf spot disease (ALS) is caused by Pseudomonas syringae pv. lachrymans ( Psl ), which mainly infects the leaves and results in limited vein and necrotic tissues [ 37 , 38 ]. In addition, cucumber fusarium wilt, one of the major fungal diseases, is a soil-borne disease caused by Fusarium oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Exocyst Complex Subunit Exo70 Gene Family in Cucumber

Liu

Zhang

et al. 2023

IJMS

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Cucumber (Cucumis sativus L.) is an important vegetable worldwide, but its yield is affected by a wide range of pathogens and pests. As the major subunit of the exocyst complex, the roles of Exo70 members have been shown in Arabidopsis and rice, but their function are unknown in cucumber. Here, we identified 18 CsExo70 members in cucumber, which were divided into three groups (Exo70.1–Exo70.3) and nine subgroups (Exo70A–Exo70I) based on the phylogenetic tree. Subsequently, systematical analyses were performed, including collinearity, gene structure, cis-acting elements, conserved motifs, expression patterns, and subcellular localization. Our results showed that CsExo70 genes were generally expressed in all tissues, and CsExo70C1 and CsExo70C2 were highly expressed in the stamen. Moreover, the expression levels of most CsExo70 genes were induced by Pseudomonas syringae pv. lachrymans (Psl) and Fusarium oxysporum f. sp. cucumerinum Owen (Foc), especially CsExo70E2 and CsExo70H3. In addition, these CsExo70s displayed similar location patterns with discrete and punctate signals in the cytoplasm. Together, our results indicate that CsExo70 members may be involved in plant development and resistance, and provide a reference for future in-depth studies of Exo70 genes in cucumber.

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“…Studies have found two major quantitative trait loci psl5.1 and psl5.2 related to disease severity through genetic mapping of the Psl locus, which may be suitable to support selection for angular leaf spot resistance (Słomnicka et al, 2018). Słomnicka et al (2021) found reactive oxygen species (ROS) in managing early defense response of cucumber to Psl , and some genes involved in host‐specific response to the highly virulent Psl strain. Pazarlar et al (2021) reported that exogenous pipecolic acid induced systemic acquired resistance (SAR) in cucumber against Psl .…”

Section: Introductionmentioning

confidence: 99%

Exogenous melatonin improves the resistance to cucumber bacterial angular leaf spot caused by Pseudomonas syringae pv. Lachrymans

Wang

et al. 2022

Physiologia Plantarum

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Pseudomonas syringae pv. Lachrymans (Psl) is a bacterial pathogen that causes cucumber bacterial angular leaf spot (BALS). It is known that melatonin (MT), as a pleiotropic signal molecule, can improve plant stress tolerance, but less information is available about the function of MT on plant resistance to bacteria disease. Here, we investigated the effect of MT on cucumber BALS. Our results show that MT inhibited the bacteria Psl growth significantly in vitro and attenuated cucumber BALS remarkably in vivo. The concentration of bacteria in leaves treated with 0.1 mM MT was approximately 10,000 times reduced at 5 days‐post‐inoculation (dpi), compared to the control without MT. Transcriptomic analysis showed that 225 differentially expressed genes (DEGs) were induced in leaves after just MT treatment for 3 h. The functions of these DEGs were mainly associated with hormone signal transduction, mitogen‐activated protein kinase (MAPK) signaling pathway, and photosynthesis, suggesting that MT could regulate plant growth and induce plant immunity. Moreover, 665 DEGs were induced when leaves were treated with exogenous MT in combination with the bacteria inoculation for 12 h. The functions of these DEGs were much related to plant–pathogen interaction, hormone signal transduction, and amino acids biosynthesis pathways. Many MT‐induced DEGs were involved in some distinct signal transduction pathways, such as calmodulin (CaM), polyamines (PAs), nitric oxide (NO), and salicylic acid (SA). The physiological analysis shows that exogenous MT spray reduced the stomatal aperture and enhanced the activities of antioxidant and defense enzymes, which were in consistent with the results of the transcriptome analysis. In addition, MT may function in regulating the metabolic balance between plant growth and defense. In conclusion, our results demonstrate that MT could alleviate the cucumber BALS via inhibiting propagation and invasion of Psl, activating plant signaling, enhancing antioxidative and defense systems, inducing stress‐related genes expression, and regulating the plant growth‐defense balance.

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Transcriptome Profiling of Cucumber (Cucumis sativus L.) Early Response to Pseudomonas syringae pv. lachrymans

Cited by 9 publications

References 67 publications

Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement

Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement

Genome-Wide Analysis of Exocyst Complex Subunit Exo70 Gene Family in Cucumber

Exogenous melatonin improves the resistance to cucumber bacterial angular leaf spot caused by Pseudomonas syringae pv. Lachrymans

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