Transcriptome profiling in oral cavity and esophagus tissues from (<i>S</i>)‐N′‐nitrosonornicotine‐treated rats reveals candidate genes involved in human oral cavity and esophageal carcinogenesis

Khammanivong, Ali; Anandharaj, Arunkumar; Qian, Xuemin; Song, Jung Min; Upadhyaya, Pramod; Balbo, Silvia; Bandyopadhyay, Dipankar; Dickerson, Erin B.; Hecht, Stephen S.; Kassie, Fekadu

doi:10.1002/mc.22459

Cited by 6 publications

(6 citation statements)

References 52 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…13 Very recently, AIRE expression was reported in human and mouse keratinocytes and in tumors originating in stratified and pseudostratified epithelia, as head and neck squamous carcinomas. 16,17 Thus, the shared feature of promiscuous expression of some genes by mTECs and cancer cells 18,19 raises the possibility that analogous mechanisms of transcriptional regulation operate in both cell types and that AIRE, as part of or a consequence of such mechanisms, is ectopically activated in some solid human tumors.…”

Section: Introductionmentioning

confidence: 99%

Expression and prognostic significance of the autoimmune regulator gene in breast cancer cells

et al. 2016

View full text Add to dashboard Cite

The autoimmune regulator gene (AIRE) plays a fundamental role in tolerance by promoting the expression of tissue-specific antigens in medullary thymic epithelial cells (mTECs). Recently, AIRE expression was detected also in human keratinocytes and in tumors originating in stratified epithelia. Here, we tested whether AIRE is expressed in cancer cells. We analyzed AIRE expression in cancer cases from The Cancer Genome Atlas (TCGA) RNA-seq dataset and we found association with better outcome. AIRE protein expression was verified by immunohistochemistry in a cohort of 39 human breast cancer specimens and its prognostic relevance was confirmed in microarray-based gene expression data set NKI-295 and KM-Plotter. Both in the RNA-seq and gene expression datasets analyzed, AIRE expression was an independent strong prognostic factor for relapse-free survival (RFS), particularly in estrogen receptor-positive tumors. Enrichment of translation-related pathways was observed in AIRE-expressing tumors by Ingenuity Pathway Analysis and a significant increase of cells in G1 phase and activation of caspase cascades was induced by AIRE transfection in breast cancer luminal cell lines, suggesting that AIRE-induced over-translation of proteins lead to cycle arrest and apoptosis. These data are the first to identify AIRE expression in breast cancer and an association with prognosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression and prognostic significance of the autoimmune regulator gene in breast cancer cells

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Only two patients with stage II or stage III ESCCs that did not have preceding neoadjuvant therapies were enrolled in this study; accordingly, we predicted that the number of enrolled patients might not be enough to meet a statistical significance. Instead, we enrolled patients with stage II or III OSCCs in the study, because they likely had similar TMEs to ESCCs, and they shared the same pathologic diagnoses and carcinogenic pathways as described above [ 26 , 27 , 28 ]. Besides, patients with stage II or III OSCCs are usually treated by surgery alone, without any preceding neoadjuvant therapies.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests the difficulty of enrolling a sufficient number of patients with advanced ESCCs that have been solely treated by surgery as a control group. Instead, we enrolled patients with oral squamous cell carcinoma (OSCC) in this study; OSCC is considered to be comparable to ESCC, because they share similar pathologic diagnoses, squamous cell carcinoma, carcinogenic pathways (such as alcohol drinking and smoking), and molecular features [ 26 , 27 , 28 , 29 ]. Additionally, ESCC is usually treated by neoadjuvant CRT or chemotherapy followed by surgery, as described above, whereas OSCC is rarely treated with neoadjuvant chemotherapy or CRT before surgery.…”

Section: Introductionmentioning

confidence: 99%

Clonal Expansion of Tumor-Infiltrating T Cells and Analysis of the Tumor Microenvironment within Esophageal Squamous Cell Carcinoma Relapsed after Definitive Chemoradiation Therapy

Mori

Kumagai

Nasu

et al. 2021

IJMS

View full text Add to dashboard Cite

(1) Background: Comparable prognoses after definitive chemoradiation therapy (CRT) to surgery alone for esophageal squamous cell carcinoma (ESCC) have been previously reported; however, no robust prognostic markers have been established. The clonality of tumor-infiltrating lymphocytes (TILs) and tumor microenvironments (TMEs) in ESCC relapsed after CRT were examined to explore prognostic markers. (2) Methods: Clonality of TIL and TME were examined in ESCC with and without preceding CRT, as well as oral squamous cell carcinoma (OSCC) and healthy volunteers as controls. The clonality of TIL was assessed by T-cell receptor (TCR) α and β repertoire analyses and evaluated by diversity indices. The TME was assessed by quantitative polymerase chain reaction evaluating PD-L1 and CD8B. (3) Results: The clonal expansion of TIL was significantly induced within ESCCs and OSCCs, when compared to healthy volunteers, and was mostly induced within ESCCs after definitive CRT. Diversity indices of TIL were not associated with the prognosis, but the ratio of PD-L1 mRNA to CD8B mRNA in TME was significantly associated with a poor prognosis after salvage surgery (p = 0.007). (4) Conclusions: The clonal expansion of TIL is induced after definitive CRT for ESCC, and the ratio of PD-L1 mRNA to CD8B mRNA within tumor tissues is a prognostic marker candidate for salvage esophagectomy after CRT.

show abstract

“…FETUB, a liver-derived plasma protein, has recently been reported to influence glucose metabolism (36). FETUB copy number amplification in human esophageal cancer, head and neck squamous cell carcinoma was at least 10–23% (37). FETUB was associated with decreased lung function in patients with chronic obstructive pulmonary disease (COPD), and predicted the occurrence of acute exacerbation or frequent acute exacerbation (38).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics and functional analyses of key genes in smoking‑associated lung adenocarcinoma

et al. 2019

View full text Add to dashboard Cite

Smoking is one of the most important factors associated with the development of lung cancer. However, the signaling pathways and driver genes in smoking-associated lung adenocarcinoma remain unknown. The present study analyzed 433 samples of smoking-associated lung adenocarcinoma and 75 samples of non-smoking lung adenocarcinoma from the Cancer Genome Atlas database. Gene Ontology (GO) analysis was performed using the Database for Annotation, Visualization and Integrated Discovery and the ggplot2 R/Bioconductor package. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed using the R packages RSQLite and org.Hs.eg.db. Multivariate Cox regression analysis was performed to screen factors associated with patient survival. Kaplan-Meier and receiver operating characteristic curves were used to analyze the potential clinical significance of the identified biomarkers as molecular prognostic markers for the five-year overall survival time. A total of 373 differentially expressed genes (DEGs; |log2-fold change|≥2.0 and P<0.01) were identified, of which 71 were downregulated and 302 were upregulated. These DEGs were associated with 28 significant GO functions and 11 significant KEGG pathways (false discovery rate <0.05). Two hundred thirty-eight proteins were associated with the 373 differentially expressed genes, and a protein-protein interaction network was constructed. Multivariate regression analysis revealed that 7 mRNAs, cytochrome P450 family 17 subfamily A member 1, PKHD1 like 1, retinoid isomerohydrolase RPE65, neurotensin receptor 1, fetuin B, insulin-like growth factor binding protein 1 and glucose-6-phosphatase catalytic subunit, significantly distinguished between non-smoking and smoking-associated adenocarcinomas. Kaplan-Meier analysis demonstrated that patients in the 7 mRNAs-high-risk group had a significantly worse prognosis than those of the low-risk group. The data obtained in the current study suggested that these genes may serve as potential novel prognostic biomarkers of smoking-associated lung adenocarcinoma.

show abstract

Transcriptome profiling in oral cavity and esophagus tissues from (S)‐N′‐nitrosonornicotine‐treated rats reveals candidate genes involved in human oral cavity and esophageal carcinogenesis

Cited by 6 publications

References 52 publications

Expression and prognostic significance of the autoimmune regulator gene in breast cancer cells

Expression and prognostic significance of the autoimmune regulator gene in breast cancer cells

Clonal Expansion of Tumor-Infiltrating T Cells and Analysis of the Tumor Microenvironment within Esophageal Squamous Cell Carcinoma Relapsed after Definitive Chemoradiation Therapy

Bioinformatics and functional analyses of key genes in smoking‑associated lung adenocarcinoma

Contact Info

Product

Resources

About