Transcriptome profiling disclosed the effect of single and combined drought and heat stress on reprogramming of genes expression in barley flag leaf

Mikołajczak, Krzysztof; Kuczyńska, Anetta; Krajewski, Paweł; Kempa, Michał; Nuc, Maria

doi:10.3389/fpls.2022.1096685

Cited by 9 publications

(26 citation statements)

References 107 publications

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“…It was observed that the number of genes specifically upregulated in flc mutants under the combination of salinity and heat were significantly higher (817) than those found in Wt (547) (Tables S6 and S7). This observation has been extensively reported by other researchers and highlights the importance of studying abiotic stress in combination (Lopez‐Delacalle et al., 2021; Mikołajczak et al., 2022; Rivero et al., 2014; Zandalinas, Rivero, et al., 2016). A GO enrichment analysis based on the biological processes affected showed that in both genotypes, genes related to RNA modification and protein folding were significantly enriched (Table S10).…”

Section: Resultssupporting

confidence: 80%

Specific ABA‐independent tomato transcriptome reprogramming under abiotic stress combination

Pardo‐Hernández,

Arbona,

Simón

et al. 2024

The Plant Journal

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SUMMARYCrops often have to face several abiotic stresses simultaneously, and under these conditions, the plant's response significantly differs from that observed under a single stress. However, up to the present, most of the molecular markers identified for increasing plant stress tolerance have been characterized under single abiotic stresses, which explains the unexpected results found when plants are tested under real field conditions. One important regulator of the plant's responses to abiotic stresses is abscisic acid (ABA). The ABA signaling system engages many stress‐responsive genes, but many others do not respond to ABA treatments. Thus, the ABA‐independent pathway, which is still largely unknown, involves multiple signaling pathways and important molecular components necessary for the plant's adaptation to climate change. In the present study, ABA‐deficient tomato mutants (flacca, flc) were subjected to salinity, heat, or their combination. An in‐depth RNA‐seq analysis revealed that the combination of salinity and heat led to a strong reprogramming of the tomato transcriptome. Thus, of the 685 genes that were specifically regulated under this combination in our flc mutants, 463 genes were regulated by ABA‐independent systems. Among these genes, we identified six transcription factors (TFs) that were significantly regulated, belonging to the R2R3‐MYB family. A protein–protein interaction network showed that the TFs SlMYB50 and SlMYB86 were directly involved in the upregulation of the flavonol biosynthetic pathway‐related genes. One of the most novel findings of the study is the identification of the involvement of some important ABA‐independent TFs in the specific plant response to abiotic stress combination. Considering that ABA levels dramatically change in response to environmental factors, the study of ABA‐independent genes that are specifically regulated under stress combination may provide a remarkable tool for increasing plant resilience to climate change.

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Section: Resultssupporting

confidence: 80%

Specific ABA‐independent tomato transcriptome reprogramming under abiotic stress combination

Pardo‐Hernández,

Arbona,

Simón

et al. 2024

The Plant Journal

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“…However, in the embryonal axis, the expression of this gene and the change in its protein level induced by abiotic stresses has not been investigated so far. Although the function of ns-LTP2 proteins has not been fully described, the results presented in this work, combined with those obtained by others [ 15 , 58 ], may lead to the conclusion that the expression of barley ns-LTP2 genes is subject to tissue-specific regulation. Apart from the genes belonging to ns-LTP2 expressed in the leaf or root, ns-LTP2 .…”

Section: Discussionsupporting

confidence: 63%

“…Barley ns-LTP2 group consists of eight members [ 18 ], but each of them appears to be expressed in different tissues, for example Mikołajczak [ 58 ] identified three ns-LTP2 -like genes ( ns-LTP2 . 4 , ns-LTP2 .…”

Section: Discussionmentioning

confidence: 99%

The impact of multiple abiotic stresses on ns-LTP2.8 gene transcript and ns-LTP2.8 protein accumulation in germinating barley (Hordeum vulgare L.) embryos

Kempa,

Mikołajczak,

Ogrodowicz

et al. 2024

PLoS ONE

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Abiotic stresses occur more often in combination than alone under regular field conditions limiting in more severe way crop production. Stress recognition in plants primarily occurs in the plasma membrane, modification of which is necessary to maintain homeostasis in response to it. It is known that lipid transport proteins (ns-LTPs) participate in modification of the lipidome of cell membranes. Representative of this group, ns-LTP2.8, may be involved in the reaction to abiotic stress of germinating barley plants by mediating the intracellular transport of hydrophobic particles, such as lipids, helping to maintain homeostasis. The ns-LTP2.8 protein was selected for analysis due to its ability to transport not only linear hydrophobic molecules but also compounds with a more complex spatial structure. Moreover, ns-LTP2.8 has been qualified as a member of pathogenesis-related proteins, which makes it particularly important in relation to its high allergenic potential. This paper demonstrates for the first time the influence of various abiotic stresses acting separately as well as in their combinations on the change in the ns-LTP2.8 transcript, ns-LTP2.8 protein and total soluble protein content in the embryonal axes of germinating spring barley genotypes with different ns-LTP2.8 allelic forms and stress tolerance. Tissue localization of ns-LTP2.8 transcript as well as ns-LTP2.8 protein were also examined. Although the impact of abiotic stresses on the regulation of gene transcription and translation processes remains not fully recognized, in this work we managed to demonstrate different impact on applied stresses on the fundamental cellular processes in very little studied tissue of the embryonal axis of barley.

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“…Also, it was clear from our results that the combination of salinity and heat induced a specific transcriptome response, in both Wt and flc mutants, which was completely different from that observed under salinity or heat applied individually, with 992 and 1243 specific DEGs, respectively (Figure 2a; Tables S5-S7). This observation has been extensively reported by other researchers, and highlights the importance of studying abiotic stress in combination (Rivero et al ., 2014; Zandalinas, Rivero, et al ., 2016; Lopez-Delacalle et al ., 2021; Mikołajczak et al ., 2022). However, from the specific DEGs found under the combination of salinity and heat, only 132 genes were common to Wt and flc mutants, which indicated a specific transcriptional reprogramming for ABA-deficient tomato plants, which still showed 1110 DEGs that were specifically regulated under these conditions (Figure 2a; Table S7).…”

Section: Resultsmentioning

confidence: 99%

Specific ABA-independent tomato transcriptome reprogramming under abiotic stress combination

Pardo-Hernández

Martínez-Lorente

Marti-Guillen

et al. 2023

Preprint

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Crops often have to face several abiotic stresses simultaneously, and under these conditions, the plant response significantly differs from that observed under a single stress. Nevertheless, most of the molecular markers identified for increasing plant stress tolerance have been characterized under single abiotic stresses, explaining their unexpected results when they are tested under real field conditions. One important regulator of the plant responses to abiotic stresses is ABA. The ABA signaling system engages many stress-responsive genes, however, many others do not respond to ABA treatments. Thus, the ABA-independent pathway, which is still largely unknown, involve multiple signaling pathways and important molecular components necessary for the plant adaptation to climate change. In the present study, tomato ABA-deficient mutants (flacca, flc) were subjected to salinity, heat, or their combination. A deep RNA-seq analysis revealed that the combination of salinity and heat induced an important reprogramming of the tomato transcriptome, and from the 685 genes that were specifically regulated under this combination in our flc mutants, 463 genes were regulated by ABA-independent systems. Among these genes, we identified 6 transcription factors (TFs) belonging to the R2R3MYB family that were significantly upregulated. A protein-protein interaction network showed that the TFs SlMYB50 and SlMYB86 were directly involved in the upregulation of the flavonol biosynthetic pathway-related genes. This is the first time that some important ABA-independent TFs involved in the specific plant response to abiotic stress combination have been identified. Considering that ABA levels dramatically change in response to environmental factors, the study of ABA-independent genes that are specifically regulated under stress combination may provide a marvelous tool for increasing plant resilience to climate change.

show abstract

Transcriptome profiling disclosed the effect of single and combined drought and heat stress on reprogramming of genes expression in barley flag leaf

Cited by 9 publications

References 107 publications

Specific ABA‐independent tomato transcriptome reprogramming under abiotic stress combination

Specific ABA‐independent tomato transcriptome reprogramming under abiotic stress combination

The impact of multiple abiotic stresses on ns-LTP2.8 gene transcript and ns-LTP2.8 protein accumulation in germinating barley (Hordeum vulgare L.) embryos

Specific ABA-independent tomato transcriptome reprogramming under abiotic stress combination

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