Transcriptome Profiling Data of<i>Botrytis cinerea</i>Infection on Whole Plant<i>Solanum lycopersicum</i>

Srivastava, Dhruv Aditya; Arya, Gulab Chand; Pandaranayaka, Eswari P. J.; Manasherova, Ekaterina; Prusky, Dov; Elad, Y.; Frenkel, Omer; Harel, Amnon

doi:10.1094/mpmi-05-20-0109-a

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…Transcriptional profiling during the B. cinerea infection process has been performed in several angiosperms [38][39][40][48][49][50][51]. However, no information related to the molecular mechanisms used by B. cinerea during moss invasion is available.…”

Section: Discussionmentioning

confidence: 99%

“…All primer combinations showed amplification efficiencies greater than 95%. [38], Lactuca sativa (lettuce) [39], and Solanum lycopersicum (tomato) [40], were obtained from NCBI. These plant hosts were chosen since A. thaliana represents a model plant for angiosperms, and S. lycopersicum and L. sativa are two important crops affected by B. cinerea.…”

Section: Rna Extraction Rna Sequencing Data Processing and Qpcr Anamentioning

confidence: 99%

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Botrytis cinerea Transcriptome during the Infection Process of the Bryophyte Physcomitrium patens and Angiosperms

Reboledo

Agorio

Vignale

et al. 2020

JoF

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Botrytis cinerea is a necrotrophic pathogen that causes grey mold in many plant species, including crops and model plants of angiosperms. B. cinerea also infects and colonizes the bryophyte Physcomitrium patens (previously Physcomitrella patens), which perceives the pathogen and activates defense mechanisms. However, these defenses are not sufficient to stop fungal invasion, leading finally to plant decay. To gain more insights into B. cinerea infection and virulence strategies displayed during moss colonization, we performed genome wide transcriptional profiling of B. cinerea during different infection stages. We show that, in total, 1015 B. cinerea genes were differentially expressed in moss tissues. Expression patterns of upregulated genes and gene ontology enrichment analysis revealed that infection of P. patens tissues by B. cinerea depends on reactive oxygen species generation and detoxification, transporter activities, plant cell wall degradation and modification, toxin production and probable plant defense evasion by effector proteins. Moreover, a comparison with available RNAseq data during angiosperm infection, including Arabidopsis thaliana, Solanum lycopersicum and Lactuca sativa, suggests that B. cinerea has virulence and infection functions used in all hosts, while others are more specific to P. patens or angiosperms.

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Section: Discussionmentioning

confidence: 99%

Section: Rna Extraction Rna Sequencing Data Processing and Qpcr Anamentioning

confidence: 99%

Botrytis cinerea Transcriptome during the Infection Process of the Bryophyte Physcomitrium patens and Angiosperms

Reboledo

Agorio

Vignale

et al. 2020

JoF

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“…The Botrytis cinerea transcriptome in planta was addressed in a few species, such as A. thaliana 6 , cucumber [ 21 ], kiwifruit [ 22 ], tomato, and others [ 23 ]. However, the molecular mechanisms associated with successful B. cinerea infection during the early stages of fruit ripening are unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have been trying to clarify the transcriptome landscape behind B. cinerea virulence in different species, such as cucumber and A. thaliana leaves [ 6 , 21 ], kiwifruit [ 22 ], tomato fruit, and others [ 23 ]. Moreover, the fungus transcriptome during infection of grapes (cv.…”

Section: Introductionmentioning

confidence: 99%

Virulence-related metabolism is activated in Botrytis cinerea mostly in the interaction with tolerant green grapes that remain largely unaffected in contrast with susceptible green grapes

Soares

Pimentel

Erban

et al. 2022

Horticulture Research

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Botrytis cinerea is responsible for the gray mold disease, severely affecting Vitis vinifera grapevine and hundreds of other economically important crops. However, many mechanisms of this fruit-pathogen interaction remain unknown. The combined analysis of the transcriptome and metabolome of green fruits infected with B. cinerea from susceptible and tolerant genotypes was never performed in any fleshy fruit, mostly because green fruits are widely accepted to be resistant to this fungus. In this work, peppercorn-sized fruits were infected in the field or mock-treated, and infected berries were collected at green (EL32) stage from a susceptible (Trincadeira) and a tolerant (Syrah) variety. RNAseq and GC-MS data suggested that Syrah exhibited a pre-activated/basal defense relying on specific signaling pathways, hormonal regulation, namely jasmonate and ethylene metabolisms, and linked to phenylpropanoid metabolism. In addition, putative defensive metabolites such as shikimic, ursolic/ oleanolic, and trans-4-hydroxy cinnamic acids, and epigallocatechin were more abundant in Syrah than Trincadeira before infection. On the other hand, Trincadeira underwent relevant metabolic reprogramming upon infection but was unable to contain disease progression. RNA-seq analysis of the fungus in planta revealed an opposite scenario with higher gene expression activity within B. cinerea during infection of the tolerant cultivar and less activity in infected Trincadeira berries. The results suggested an activated virulence state during interaction with the tolerant cultivar without visible disease symptoms. Together, this study brings novel insights related to early infection strategies of B. cinerea and the green berry defense against necrotrophic fungi.

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“…It has been reported that during the fungal infection process, several transcriptome changes take place, especially in the genes coding secreted proteins involved in pathogenesis [6][7][8]. In order to monitor changes in the gene expression during pathogenesis in a sensitive and specific manner, reverse transcription quantitative polymerase chain reaction (RT-qPCR) can be used.…”

Section: Introductionmentioning

confidence: 99%

Identification of reference genes and their validation for gene expression analysis in phytopathogenic fungus Macrophomina phaseolina

et al. 2022

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Macrophomina phaseolina is a soil-borne pathogenic fungus that infects a wide range of crop species and causes severe yield losses. Although the genome of the fungus has been sequenced, the molecular basis of its virulence has not been determined. Identification of up-regulated genes during fungal infection is important to understand the mechanism involved in its virulence. To ensure reliable quantification, expression of target genes needs to be normalized on the basis of certain reference genes. However, in the case of M. phaseolina, reference genes or their expression analysis have not been reported in the literature. Therefore, the objective of this study was to evaluate 12 candidate reference genes for the expression analysis of M. phaseolina genes by applying three different fungal growth conditions: a) during root and stem infection of soybean, b) in culture media with and without soybean leaf infusion and c) by inoculating a cut-stem. Based on BestKeeper, geNorm and NormFinder algorithms, CYP1 was identified as the best recommended reference gene followed by EF1β for expression analysis of fungal gene during soybean root infection. Besides Mp08158, CYP1 gene was found suitable when M. phaseolina was grown in potato-dextrose broth with leaf infusion. In the case of cut-stem inoculation, Mp08158 and Mp11185 genes were found to be most stable. To validate the selected reference genes, expression analysis of two cutinase genes was performed. In general, the expression patterns were similar when the target genes were normalized against most or least stable gene. However, in some cases different expression pattern can be obtained when least stable gene is used for normalization. We believe that the reference genes identified and validated in this study will be useful for gene expression analysis during host infection with M. phaseolina.

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Transcriptome Profiling Data ofBotrytis cinereaInfection on Whole PlantSolanum lycopersicum

Cited by 7 publications

References 27 publications

Botrytis cinerea Transcriptome during the Infection Process of the Bryophyte Physcomitrium patens and Angiosperms

Botrytis cinerea Transcriptome during the Infection Process of the Bryophyte Physcomitrium patens and Angiosperms

Virulence-related metabolism is activated in Botrytis cinerea mostly in the interaction with tolerant green grapes that remain largely unaffected in contrast with susceptible green grapes

Identification of reference genes and their validation for gene expression analysis in phytopathogenic fungus Macrophomina phaseolina

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