Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves

Park, Myoung Ryoul; Baek, Seong-Bum; Reyes, Benildo G. de los; Yun, Song Joong; Hasenstein, Karl H.

doi:10.1016/j.jplph.2011.09.002

Cited by 38 publications

(23 citation statements)

References 48 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These included transporters involved in uptake of not only macroelements such as nitrate, potassium, sulfate and calcium, but also microelements such as boron, zinc, iron and copper. It was also proposed that P nutrition could affect sugar metabolisms via likely crosstalk between the two engaged signaling pathways (Müller et al 2007;Park et al 2012). Consistent with this idea, we found that the transcription of five sugar transporters was affected by P deficiency in the Pt seedlings.…”

Section: P Deficiency Affects the Transcription Of Multiple Transporterssupporting

confidence: 88%

Transcriptome responses to phosphate deficiency in Poncirus trifoliata (L.) Raf

Bai

Chen

et al. 2014

Acta Physiol Plant

View full text Add to dashboard Cite

Phosphorus (P) is an essential macronutrient for plant growth and development; however, soil P available for plant absorption is often limited, putting constraints over agricultural sustainability. Understanding the physiological and molecular responses to P deficiency can help design strategies for diagnosis and mitigation of P deficiency in crop plants. The advent of the next-generation sequencing technologies has made it possible to characterize genome-wide molecular responses to P deficiency in plants. However, such research efforts are very limited for woody crops. In this study, RNA-seq was used to investigate P starvation-induced transcriptomic changes in roots of a frequently used citrus rootstock, Poncirus trifoliata (L.) Raf. A total of 1,135 genes showed differential expression in response to P deficiency. The transcriptomic data were further validated by real-time quantitative RT-PCR. Interestingly, at least one or more P-responsive ciselements (P1BS) were found in the promoter regions of 76 differentially expressed genes. Functional annotation revealed that the predicted proteins of 117 of the differentially expressed genes were assigned to the categories of transporters, transcription factors or components involved in plant hormone signal regulation, suggesting that these genes may play important roles in response to P starvation. A comparative analysis of the citrus-and Arabidopsisresponsive transcripts under P deficiency also identified 174 commonly regulated genes, including those involved in P metabolism. Taken together, our transcriptomic data revealed changes of genome-wide gene expression in responses to P starvation in Poncirus, which should provide a solid basis for future identification and characterization of key genes involved in nutritional stress response in citrus rootstocks.

show abstract

Section: P Deficiency Affects the Transcription Of Multiple Transporterssupporting

confidence: 88%

Transcriptome responses to phosphate deficiency in Poncirus trifoliata (L.) Raf

Bai

Chen

et al. 2014

Acta Physiol Plant

View full text Add to dashboard Cite

show abstract

“…Since past one and half decade several transcriptome analysis were performed in different plant species in response to diverse micronutrient deficiency to mine the important genes along with their role in different metabolic pathways (Wasaki et al, 2003, 2006; Li et al, 2010a,b; Cai et al, 2012; Ma et al, 2012; Park et al, 2012; Takehisa et al, 2013, 2015; Yu et al, 2016; Forieri et al, 2017; Gupta et al, 2017). In continuation, the current study reports 398 differentially expressed genes through a comparative transcriptomic study of micronutrient deficient samples grown in soil having environment growth conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The transcriptomic analysis provides genome-wide measurement of messenger RNA (mRNA) expression levels based on microarray technology. Microarray technology is rigorously used to uncover the regulatory, signaling, sensing and adaptation mechanism of transcripts in response to nutrients deficiency (mainly N, P , and K ) in model plants (Wasaki et al, 2003, 2006; Wu et al, 2003; Armengaud et al, 2004; Misson et al, 2005; Lian et al, 2006; Li et al, 2010a,b; Krapp et al, 2011; Cai et al, 2012; Ma et al, 2012; Park et al, 2012). These in-vitro grown customized experiments elucidate that several transcriptional responses occur when plants were subjected to various nutrients deficiency.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Soil Grown T. aestivum cv. Root to Reveal the Changes in Expression of Genes in Response to Multiple Nutrients Deficiency

et al. 2017

View full text Add to dashboard Cite

Deficiency of necessary macronutrients, i.e., Potassium (K), Magnesium (Mg), Nitrogen (N), Phosphorus (P), and Sulfate (S) in the soil leads to a reduction in plant growth and yield, which is a result of changes in expression level of various genes. This study was performed to identify the differentially expressed genes and its associated metabolic pathways occurred in soil grown wheat root samples excavated from the control and treated fields. To identify the difference in gene expression levels due to deficiency of the said nutrients, a transcriptomic, meta-analysis was performed on array expression profile data. A set of 435 statistically significant probes encoding 398 Nutrient Deficiency Response Genes (NRGs) responding at-least one nutrients deficiency (ND) were identified. Out of them 55 NRGs were found to response to minimum two ND. Singular Enrichment Analysis (SEA) predicts ontological based classifications and functional analysis of NRGs in different cellular/molecular pathways involved in root development and growth. Functional annotation and reaction mechanism of differentially expressed genes, proteins/enzymes in the different metabolic pathway through MapMan analysis were explored. Further the meta-analysis was performed to revels the active involvement each NRGs in distinct tissues and their comparative potential expression analysis in different stress conditions. The study results in exploring the role of major acting candidate genes such as Non-specific serine/threonine protein kinase, Xyloglucan endotransglucosylase/hydrolase, Peroxides, Glycerophosphoryl diester phosphodiesterase, S-adenosylmethionine decarboxylase proenzyme, Dehydrin family proteins, Transcription factors, Membrane Proteins, Metal binding proteins, Photosystem proteins, Transporter and Transferase associated in different metabolic pathways. Finally, the differences of transcriptional responses in the soil-grown root of T. aestivum cv. and in-vitro grown model plants under nutrients deficiency were summarized.

show abstract

“…Results showed that the availability of P in soil increased from the beginning of the experiment (16 mg dm -3 ) to P fertilizer applications, promoting changes in the deposition of reducing sugars in the 'FHIA 18' unripe fruits. This outcome may have occurred because carbohydrates synthesis in plants are influenced by P content in plant tissue, which is confirmed by Park et al (2012), who found that the lowest application of P fertilizer decreases glucose biosynthesis. After the first cycle, the lack of response to P is due to the increase in root system, which may have enabled the absorption of P in deeper soil layers, as the cycles progress (J. T. A.…”

Section: Physicochemical Characterization From Unripe Fruits Of Bananasmentioning

confidence: 65%

“…Starch synthesis in plants are highly influenced by phosphorus content in their tissue (Park, Baek, Reyes, Yun, & Hasenstein, 2012;Zhao et al, 2015). Starch is the main source of carbohydrate in unripe bananas, its content varies widely between and within botanical species, even in the same cultivar when grown under different conditions (Mesquita et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characteristics of unripe and ripe banana ‘FHIA 18’ submitted to phosphorus fertilizer over three production cycles

Bolfarini

Souza

Putti

et al. 2020

SCA

View full text Add to dashboard Cite

The banana fruits of 'FHIA 18' have sensory properties like the 'Prata' ones. However, there is no information regarding to phosphorus (P) requirement of this hybrid and its effects on postharvest quality. This study aims to evaluate the physicochemical properties of 'FHIA 18' banana fruits submitted to different phosphorus levels under the edaphoclimatic conditions of the municipality of São Manuel, state of São Paulo, during three successive production cycles. Therefore, different levels of Phosphorus (0 [0%], 20 [50%)], 30 [75%], 40 [100%], 50 [125%] and 60 kg ha -1 year -1 P 2 O 5 [150%]) were applied during three production cycles. A completely randomized design and split plot structure was used, the plots consisted of six phosphorus fertilizers doses and three production cycles (2014, 2015 and 2016) in sub plots. For unripe fruits, we evaluated the contents of dry matter, starch, total sugars, reducing sugars and phosphorus. For ripe fruits, we analyzed firmness, peel colour descriptors, total titratable acidity, total soluble solids, maturity index, total sugars and reducing sugars. Results showed that P fertilizer provided an increase in P content in unripe fruits until the application of 100% of the recommended dose; besides an increase in the reducing sugar contents until the application of 61% over the first production cycle. Results also indicated that applying lower phosphorus doses (˂100% of the recommended dose) promoted lower total titratable acidity and higher maturity index (52-53% of recommendation) in ripe fruits. Regardless of P 2 O 5 dose, the contents of dry matter, starch and phosphorus increased in unripe fruits from first to third crop cycle, as well as the firmness, total soluble solids contents, maturity index and sugar content in ripe fruits. ResumoOs frutos da bananeira 'FHIA 18' possuem características sensoriais semelhantes à banana 'Prata', porém, não existem informações sobre as reais necessidades em fósforo (P) deste híbrido e seus efeitos Semina: Ciências Agrárias, Londrina, v. 41, n. 1, p. 33-48, jan./fev. 2020 Bolfarini, A. C. B. et al.na produção e qualidade pós-colheita. O objetivo deste trabalho foi avaliar o perfil físico-químico dos frutos da bananeira 'FHIA 18' submetida à níveis de adubação fosfatada, em São Manuel-SP, durante três ciclos sucessivos. Os níveis de adubação com P 2 O 5 aplicados foram: sem adubação com P (0%); 20 kg ha -1 ano -1 (50%); 30 kg ha -1 ano -1 (75%); 40 kg ha -1 ano -1 (100%); 50 kg ha -1 ano -1 (125%); e 60 kg ha -1 ano -1 (150%). Utilizou-se o delineamento inteiramente casualizado, em arranjo de parcelas subdivididas, sendo as parcelas compostas pelos seis níveis de adubação com fósforo e as subparcelas pelos três ciclos de produção (2014, 2015 e 2016). Foram avaliados nos frutos verdes os teores de matéria seca, amido, açúcares totais, açúcares redutores e de fósforo. Nos frutos maduros avaliou-se a firmeza, os descritores de coloração da casca, acidez titulável total, sólidos solúveis totais, índice de maturação, açúcares totais e açúcare...

show abstract

Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves

Cited by 38 publications

References 48 publications

Transcriptome responses to phosphate deficiency in Poncirus trifoliata (L.) Raf

Transcriptome responses to phosphate deficiency in Poncirus trifoliata (L.) Raf

Transcriptomic Analysis of Soil Grown T. aestivum cv. Root to Reveal the Changes in Expression of Genes in Response to Multiple Nutrients Deficiency

Physicochemical characteristics of unripe and ripe banana ‘FHIA 18’ submitted to phosphorus fertilizer over three production cycles

Contact Info

Product

Resources

About