Transcriptome profiling analysis reveals involvement of SAM cycle and methionine pathway in low potassium tolerance in barley

Comparative Analysis of Transcriptome Profiles Reveals the Mechanisms in the Difference of Low Potassium Tolerance among Cultivated and Tibetan Wild Barleys

“…Moreover, functional analysis showed that KAT1, KAT2, and GORK were basically expressed in the guard cells, and synergistically mediated the inward or outward potassium ion flow [29][30][31][32]. This result was consistent with our recent finding that GORK was up-regulated in the XZ153′ leaves under LK stress [11]. Moreover, Ca 2+ is an important second messenger responding to various biotic and abiotic stresses in plants, including nutrient deficiency [33][34][35].…”

“…Our previous studies proved that some Tibetan wild barley genotypes had high LK tolerance [8][9][10]. Moreover, we found that XZ153, an LK-tolerant wild genotype, could remobilize more K from the older leaves into the younger ones than ZD9, an LK-sensitive genotype [11]. Additionally, XZ153 can absorb and accumulate more K to maintain its LK tolerance [12].…”

“…At 48 h and 15 d after LK stress treatment, shoots of seedlings in all treatments and replicates [total 36, 3 genotypes × 2 treatments × 2 sampling times × 3 biological replications] were sampled for RNA-Seq. Additionally, the selection of the two sampling time points (48 h and 15 d) was in reference and summary to the team's previous research on the transcriptome of barley in response to LK stress [11,12]. Additionally, total RNA was extracted from the harvested shoots using Trizol reagent (Invitrogen, Carlsbad, CA, USA), then the kit NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB#E7490 (New England Biolabs, Inc., Ipswich, MA, USA), was used to isolate mRNA from 1 µg of total RNA.…”

“…The library was sequenced by using an Illumina HiSeqXten platform with a paired-end sequencing length of 150 bp (PE150) at Biomarker Technologies (Beijing, China). All the above steps were conducted according to Ye et al [11]. Lastly, pure PCR products were obtained, and library quality was further assessed.…”

Omics-driven crop potassium use efficiency breeding

“…In soybeans, the total K accumulation per plant (mg/plant) of the low-K-tolerant variety is 2.36 times that of the low-K-sensitive variety under the K deficiency condition ( Wang et al., 2012 ). In barley, the low-K-tolerant variety has a significantly higher shoot and root K concentration (mg/g dry weight) and K accumulation than the low-K-sensitive variety under the K deficiency condition ( Ye et al., 2021 ). The same phenomena have been observed in other species like rice ( Yang et al., 2004 ), maize ( Cao et al., 2007 ), wheat ( Zhao et al., 2020 ) and cotton ( Tian et al., 2008 ).…”