Transcriptome profiling analysis of uterus during chicken laying periods

Sun, Tiantian; Cong, Xiao; Yang, Zhuliang; Deng, Jixian; Yang, Xiurong

doi:10.1186/s12864-023-09521-z

Cited by 3 publications

(2 citation statements)

References 83 publications

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“…Cluster 6 exhibited high expression of THBS1, DCN [25], FRZB, COL1A2, COL3A1, MGP, GSN, and TNXB. These fibroblasts displayed heightened levels of collagen type I alpha 2 chain, thrombospondin 1, decorin, frizzled-related protein, collagen type III alpha 1 chain, matrix Gla protein, gelsolin, and tenascin XB, all of which are pivotal components of the ECM [26]. Cluster 23, on the other hand, demonstrated elevated expression of POSTN [27] and CLDN2, contributing to the production of periostin and claudin 2, which play significant roles in cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

Cell Heterogeneity Analysis Revealed the Key Role of Fibroblasts in the Magnum Regression of Ducks

Du,

Xu,

Liu

et al. 2024

Animals

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Duck egg production, like that of laying hens, follows a typical low–peak–low cycle, reflecting the dynamics of the reproductive system. Post-peak, some ducks undergo a cessation of egg laying, indicative of a regression process in the oviduct. Notably, the magnum, being the longest segment of the oviduct, plays a crucial role in protein secretion. Despite its significance, few studies have investigated the molecular mechanisms underlying oviduct regression in ducks that have ceased laying eggs. In this study, we conducted single-cell transcriptome sequencing on the magnum tissue of Shaoxing ducks at 467 days of age, utilizing the 10× Genomics platform. This approach allowed us to generate a detailed magnum transcriptome map of both egg-laying and ceased-laying ducks. We collected transcriptome data from 13,708 individual cells, which were then subjected to computational analysis, resulting in the identification of 27 distinct cell clusters. Marker genes were subsequently employed to categorize these clusters into specific cell types. Our analysis revealed notable heterogeneity in magnum cells between the egg-laying and ceased-laying ducks, primarily characterized by variations in cells involved in protein secretion and extracellular matrix (ECM)-producing fibroblasts. Specifically, cells engaged in protein secretion were predominantly observed in the egg-laying ducks, indicative of their role in functional albumen deposition within the magnum, a phenomenon not observed in the ceased-laying ducks. Moreover, the proportion of THY1+ cells within the ECM-producing fibroblasts was found to be significantly higher in the egg-laying ducks (59%) compared to the ceased-laying ducks (24%). Similarly, TIMP4+ fibroblasts constituted a greater proportion of the ECM-producing fibroblasts in the egg-laying ducks (83%) compared to the ceased-laying ducks (58%). These findings suggest a potential correlation between the expression of THY1 and TIMP4 in ECM-producing fibroblasts and oviduct activity during functional reproduction. Our study provides valuable single-cell insights that warrant further investigation into the biological implications of fibroblast subsets in the degeneration of the reproductive tract. Moreover, these insights hold promise for enhancing the production efficiency of laying ducks.

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Section: Discussionmentioning

confidence: 99%

Cell Heterogeneity Analysis Revealed the Key Role of Fibroblasts in the Magnum Regression of Ducks

Du,

Xu,

Liu

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

“…The Wannan Chickens is a local breed of chicken native to China, with a distribution that extends along the river in southern and eastern Anhui. In recent years, transcriptome analysis has been employed extensively applied to study of various species and traits ( Choi, et al, 2020 ; Cassar-Malek, et al, 2022 ; Jin, et al, 2022 ), Furthermore, it has been successfully employed in the study of poultry traits ( Ren, et al, 2021 ; Pham, et al, 2022 ; Sun, et al, 2023 ). Weighted gene co-expression network analysis ( WGCNA ) has also been widely used to identify key modules of traits and important genes in a multitude of species ( Fan, et al, 2023 ; Yu, et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%