Transcriptome profiling analysis of rare minnow (Gobiocypris rarus) gills after waterborne cadmium exposure

Wang, Zhijian; Liu, X. H.; Jin, Li; Pu, Deyong; Huang, Jing; Zhang, Yaoguang

doi:10.1016/j.cbd.2016.05.003

Cited by 15 publications

(7 citation statements)

References 76 publications

(76 reference statements)

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“…Although the complete mitochondrial genome has been reported [ 25 – 27 ], available sequences in S . sinensis were still limited compared to other Gobioninae fish species, such as Gobiocypris rarus [ 28 ], Hemibarbus maculates [ 29 ], Coreius heterodon [ 30 ], etc.…”

Section: Introductionmentioning

confidence: 99%

De novo assembly, characterization and annotation for the transcriptome of Sarcocheilichthys sinensis

et al. 2017

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The Chinese lake gudgeon Sarcocheilichthys sinensis is a small cyprinid fish with great aquaculture potential both for its edible and ornamental values. Nevertheless, available genomic and transcriptomic information for this fish is extremely deficient. In this study, a normalized cDNA library was constructed using 13 mixed tissues of an adult male S. sinensis, and was sequenced by the Illumina HiSeq2500 platform. De novo assembly was performed using 38,911,511 obtained clean reads, and a total of 147,282 unigenes with an average length of 900 bp were finally achieved. 96.2% of these unigenes were annotated in 9 public databases, and 16 segments of growth-related genes were identified for future studies. In addition, 28,493 unigenes were assigned to 61 subcategories of Gene Ontology (GO), and 10,483 unigenes were assigned to 25 categories of Cluster of Orthologous Group (COG). Moreover, 14,943 unigenes were classified into 225 pathways of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 30,666 microsatellites were detected from 17,627 unigenes with an average distribution density of 1:2405 bp. This transcriptome data set will be valuable for researches on discovery, expression and evolution on genes of interest. Meanwhile, the identified microsatellites would be useful tools for genetic and genomic studies in S. sinensis.

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Section: Introductionmentioning

confidence: 99%

De novo assembly, characterization and annotation for the transcriptome of Sarcocheilichthys sinensis

et al. 2017

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“…This study intended to address the acute toxicity of CMC-FeS on zebrafish. Therefore, the time resolved response of oxidative stress and other stress conditions, for instance, cell cycle damage, were confined within 96 h. Similar approach was employed by others 44 – 46 . Interestingly, our results indicated that CMC-FeS can cause up-regulation of some biomarker genes for inflammation and oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide Nanoparticles

Zheng

Lü

2018

Sci Rep

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Increasing utilization of stabilized iron sulfides (FeS) nanoparticles implies an elevated release of the materials into the environment. To understand potential impacts and underlying mechanisms of nanoparticle-induced stress, we used the transcriptome sequencing (RNA-seq) technique to characterize the transcriptomes from adult zebrafish exposed to 10 mg/L carboxymethyl cellulose (CMC) stabilized FeS nanoparticles for 96 h, demonstrating striking differences in the gene expression profiles in liver. The exposure caused significant expression alterations in genes related to immune and inflammatory responses, detoxification, oxidative stress and DNA damage/repair. The complement and coagulation cascades Kyoto encyclopedia of genes and genomes (KEGG) pathway was found significantly up-regulated under nanoparticle exposure. The quantitative real-time polymerase chain reaction using twelve genes confirmed the RNA-seq results. We identified several candidate genes commonly regulated in liver, which may serve as gene indicators when exposed to the nanoparticles. Hepatic inflammation was further confirmed by histological observation of pyknotic nuclei, and vacuole formation upon exposure. Tissue accumulation tests showed a 2.2 times higher iron concentration in the fish tissue upon exposure. This study provides preliminary mechanistic insights into potential toxic effects of organic matter stabilized FeS nanoparticles, which will improve our understanding of the genotoxicity caused by stabilized nanoparticles.

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“…Our results suggested that the gills are the most sensitive organ for inflammatory processes. Taking into account the higher bioaccumulation of Cd, Cu, and Zn, this might lead to the induction of inflammation and upregulation of pro-inflammatory TNF-α cytokine [40]. Western blot analysis indicated that the highest TNF-α level was recorded for the carnivorous species (BB), being in agreement with the histopathological damages.…”

Section: Heavy Metal Bioaccumulation Activates Inflammatory Pathways In the Gills Liver And Kidney Of Bb Cn And Scmentioning

confidence: 91%

Correlation between Heavy Metal-Induced Histopathological Changes and Trophic Interactions between Different Fish Species

et al. 2021

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This study assessed the distribution of heavy metals in the gills, kidney, and liver, correlated with the severity of histopathological changes, of three fish species with different feeding habitats (Barbus barbus, Squalius cephalus, and Chondrostoma nasus) from the Crișul Negru river, Romania. The levels of copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb), and zinc (Zn) in fish tissues were measured by atomic absorption spectrophotometry. Histopathology and the expressions of TNF-α and proliferation cell nuclear antigen (PCNA) were investigated by immunohistochemistry and Western blot. Our data suggest a significant correlation between the bioconcentration level of metals and structural changes. The carnivorous species was the most affected compared to the omnivorous and herbivorous ones, and the most affected organ was the kidney. Moreover, the correlation of tissue damage with the PCNA and TNF-α expression levels revealed that the herbivorous species presented less extended lesions, likely due to higher activated repair mechanisms and lower levels of inflammation. In conclusion, our data and the subsequent statistical analysis suggest that feeding behavior could be correlated with the histopathological alterations and might be used for a more profound evaluation of aquatic environment safety and analysis of aquatic ecosystems.

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Transcriptome profiling analysis of rare minnow (Gobiocypris rarus) gills after waterborne cadmium exposure

Cited by 15 publications

References 76 publications

De novo assembly, characterization and annotation for the transcriptome of Sarcocheilichthys sinensis

De novo assembly, characterization and annotation for the transcriptome of Sarcocheilichthys sinensis

Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide Nanoparticles

Correlation between Heavy Metal-Induced Histopathological Changes and Trophic Interactions between Different Fish Species

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