Transcriptome Profiling Analysis of Phosphate-Solubilizing Mechanism of Pseudomonas Strain W134

Abstract

Phosphate-solubilizing bacteria (PSB) can alleviate available phosphorus deficiency without causing environmental pollution, unlike chemical phosphate fertilizers. However, the phosphate solubilization mechanisms of PSB are still unclear. Transcriptome sequencing was used to analyze the expression patterns of differential expressed genes (DEGs) of the phosphate-solubilizing bacterium W134 under the conditions of soluble phosphorus (group A), insoluble phosphorus (group B), and lacking phosphorus (group C). Nine DEGs in three different groups were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Then, high performance liquid chromatography (HPLC) was applied to detect the concentrations and composition of organic acids. Compared with group A, Gene Ontology (GO) annotation showed that the cluster of W134 DEGs in groups B and C were basically the same. Besides, the results of enrichment Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway indicated that genes in the Citrate cycle (TCA cycle) pathway closely related to organic acid production were significantly upregulated. The qRT-PCR results were almost consistent with the expression trends of the transcriptome data. The HPLC results showed that the formic acid, ascorbic acid, acetic acid, citric acid, and succinic acid concentrations were significantly increased in group B and C (p < 0.05), while the contents of lactic acid and malic acid were significantly increased in group B (p < 0.05). The above results provided further validation that the upregulated genes should be related to W134 secretion of organic acids. Our study revealed several potential candidate genes and tried to explain phosphate solubilization mechanisms. This provides a new insight for calcareous reclaimed soil, and it will reduce the need of chemical phosphate fertilizers to promote environmentally friendly agriculture.

Moderate organic fertilizer substitution for partial chemical fertilizer improved soil microbial carbon source utilization and bacterial community composition in rain-fed wheat fields: current year

“…Phosphorus-solubilizing bacteria can transform insoluble phosphorus, which is difficult for plants to absorb and utilize, into effective forms and improve the utilization efficiency of soil phosphorus (Kalayu, 2019 ). Pseudomonas is agricultural soil's main phosphorus-solubilizing bacteria (PSB) (Wang et al, 2022 ). Soil AP content was closely related to soil bacterial community and wheat yield, indicating that soil phosphorus was an important factor driving soil microbial community and wheat yield changes (Chen et al, 2021 ).…”

Effect of ammonium stress on phosphorus solubilization of a novel marine mangrove microorganism Bacillus aryabhattai NM1-A2 as revealed by integrated omics analysis

“…4, the increasing content of acetic acid indicated that the activity of TCA cycle was limited under NH 4 + stress. Recent studies have shown that different P sources in uenced the types of organic acids [51]. Arvind et al found that the generation of formic acid was limited to the dissolution of TCP, and oxalic acid production to the solubilization of MRP, URP and NCRP [52].…”

Effect of ammonium stress on phosphorus solubilization of a novel marine mangrove microorganism Bacillus aryabhattai NM1-A2 as revealed by integrated omics analysis

“…The production of succinic acid was related to PS activity of Bacillus megaterium , while oxalic acid and citric acid were the main organic acids released by Pseudomonas sp [ 3 ]. The secretion of organic acids was also influenced by the types and concentrations of P sources [ 47 ]. Arvind et al found that the generation of formic acid was limited to the dissolution of TCP, and oxalic acid production to the solubilization of Mussoorie rock phosphate, Udaipur rock phosphate, and North Carolina rock phosphate [ 48 ].…”