Transcriptome profiles of pre‐pubertal and adult in vitro matured ovine oocytes obtained from FSH‐stimulated animals

Chen, Ying; Wu, Yihan; Lin, Jianghai; Wang, Liqin; Li, Xin; Liu, Mingjun; Huang, Jianqiang

doi:10.1111/rda.13951

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…PRELID3B has an important role in excessive melanin deposition ( 61 ) and regulates lipid accumulation in mitochondria ( 62 ). In lamb MII oocytes, down-regulation of ATP5E and up-regulation of CUL1 , MARCH7 , and TRIM17 might cause low competence of lamb embryos ( 63 ). Furthermore, it has been reported that ATP5E is a promising candidate biomarker for oocyte viability after IVM ( 64 ), so the gene might be associated with reproductive traits in female animals.…”

Section: Discussionmentioning

confidence: 99%

Whole-genome resequencing of native and imported dairy goat identifies genes associated with productivity and immunity

Zhao,

Mu,

Gong

et al. 2024

Front. Vet. Sci.

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Understanding the differences in genetic variation between local Chinese dairy goat breeds and imported breeds can help germplasm innovation and molecular breeding. However, the research is limited in this area. In this study, whole-genome resequencing data from 134 individuals of both local and imported dairy goat breeds were analyzed, and their differences in genomic genetic variation, genetic diversity, and population structure were subsequently identified. We also screened candidate genes associated with important traits of dairy goats such as milk production (STK3, GHR, PRELID3B), reproduction (ATP5E), growth and development (CTSZ, GHR), and immune function (CTSZ, NELFCD). Furthermore, we examined allele frequency distributions for the genes of interest and found significant differences between the two populations. This study provides valuable resources for the study of genetic diversity in dairy goats and lays the foundation for the selective breeding of dairy goats in the future.

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Section: Discussionmentioning

confidence: 99%

Whole-genome resequencing of native and imported dairy goat identifies genes associated with productivity and immunity

Zhao,

Mu,

Gong

et al. 2024

Front. Vet. Sci.

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“…Accordingly, HUMSCs of preterm infants may be precursor cells of other cell subsets. Nestin expression was detected in C12, C6, C8, and C10, indicating that it plays a crucial role in maintaining HUMSCs in a low differentiation state and is also considered one of the markers of early cell differentiation, [19] as evidenced by the distribution of Nestin (+) cells closer to the starting point on the pseudo-curve(Fig. 4B,4C).…”

Section: Trajectory Inferencementioning

confidence: 93%

“…C1 and C14, which mainly from BMSCs, Were high expressed genes of GNB2L1, ATP5E, and PENK, associate with oxidative phosphorylation, cell growth, migration and differentiation. [19,20]C2 was high expressed of SQSTM1, KRT19, NDUFA13, relate to epithelial cell structure, cell proliferation and mitochondrial respiratory chain, [21][22][23] C3 and C16 share differential factors (NPPB, CRYAB, and ACTG2) involved in cellular ATP release, protects cardiomyocytes, and muscle regulation. [24][25][26] Differential genes in C5 (SAA1, SYNPO2, NEAT1) relate to in ammation and cancer inhibition, [27][28][29] while C6 genes (TOP2A,, MKI67, CENPF) associate with staining structure and cell proliferation.…”

Section: Differential Gene Analysismentioning

confidence: 99%

Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs Obtained from Extremely Premature Infants: Insights from Single-Cell RNA Sequencing

huang,

Qin,

Fan

et al. 2023

Preprint

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Background In our prior study, we discovered that human umbilical cord Wharton’s Jelly derived mesenchymal stem cells (HUMSCs) obtained from extremely preterm infants demonstrated superior characteristics compared to term infants, particularly regarding cell proliferation, pluripotency, and cell damage repair ability. To explore the underlying heterogeneity between these cells further, we utilized single-cell RNA sequencing (scRNA-seq) to examine their transcriptional differences and potential molecular pathways involved in this heterogeneity. Methods We conducted scRNA-seq on HUMSCs obtained from three distinct gestational ages- 22+5 weeks, 28 weeks, and 39 weeks, respectively. To assist in the analysis, we employed the scRNA-seq data from two bone marrow mesenchymal stem cells (BMSCs) samples available in existing literature as reference datasets. Subsequently, we undertook bioinformatics analysis on the obtained transcriptomic data using the R programming language. Results Upon merging the five samples, we were able to identify a total of 17 cell subpopulations with high expression of fibroblast and MSC markers. The expression of CD146 was found to be significantly higher in HUMSCs as compared to BMSCs. Moreover, we observed higher expression of Nestin+ cells in premature HUMSCs. Cell cycle analysis revealed that the majority of HUMSCs were in the G2M phase, while BMSCs were mainly in the G1 phase. Pseudotime analysis showed that HUMSCs had a lower degree of differentiation compared to BMSCs, and this decreased with increasing gestational age. Custom gene set scoring analysis revealed that the cells expressed genes related to osteogenesis, chondrogenesis, adipogenesis, stemness, immunology, and vasculogenesis; with preterm HUMSCs displaying an immunological edge. Differential gene analysis and gene enrichment analysis indicated that CD146+Nestin+ HUMSC subpopulations displayed upregulation in immune regulation, cell proliferation-related gene expression, and gene regulatory pathways. Conclusion scRNA-seq analysis revealed differences between BMSCs and HUMSCs at both preterm and term infant. Specifically, the expression of CD146+ and Nestin+ cells was significantly higher in preterm HUMSCs, which may contribute to their advantages in immune regulation, cell proliferation-related gene expression, and regulatory pathways. These findings hold great significance in advancing our understanding of the molecular mechanisms of HUMSCs and their potential applications in disease treatment, transplantation, and regenerative medicine.

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“…No differences in blastocyst development were found between stimulated and unstimulated lambs (O'Brien et al., 1997 ). Such a reduced developmental potential is related to morphological and functional alterations (Ledda et al., 2001 ; Leoni et al., 2007 ; O'Brien et al., 1996 ; Ying et al., 2021 ). In the perspective of exploiting the large JIVET potential, the availability of predictive markers on good developmental ability has been investigated.…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Embryo biotechnologies in sheep: Achievements and new improvements

Falchi

Ledda

Zedda

2022

Reprod Domestic Animals

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Assisted reproductive technologies (ARTs) play a critical role in increasing the dissemination of superior genotypes, reducing generation interval and enhancing productivity. In small ruminants, ARTs encounter limited commercial application compared with the bovine species, although the interest towards these techniques is constantly increasing. According to the International Embryo Transfer Society (IETS) Data Retrieval Report, the total sheep embryo production both in vivo derived (IVD) and in vitro produced

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Transcriptome profiles of pre‐pubertal and adult in vitro matured ovine oocytes obtained from FSH‐stimulated animals

Cited by 8 publications

References 41 publications

Whole-genome resequencing of native and imported dairy goat identifies genes associated with productivity and immunity

Whole-genome resequencing of native and imported dairy goat identifies genes associated with productivity and immunity

Advantages of Cell Proliferation and Immune Regulation in CD146+NESTIN+ HUMSCs Obtained from Extremely Premature Infants: Insights from Single-Cell RNA Sequencing

Embryo biotechnologies in sheep: Achievements and new improvements

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