Transcriptome profile of Carrizo citrange roots in response toPhytophthora parasiticainfection

Abstract

Phytophthora parasitica is one of the most widespread Phytophthora species, which is known to cause root rot, foot rot/gummosis and brown rot of fruits in citrus. In this study, we have analyzed the transcriptome of a commonly used citrus rootstock Carrizo citrange in response to P. parasitica infection using the RNA-seq technology. In total, we have identified 6692 differentially expressed transcripts (DETs) among P. parasitica-inoculated and mock-treated roots. Of these, 3960 genes were differentially expressed at 24 h post inoculation and 5521 genes were differentially expressed at 48 h post inoculation. Gene ontology analysis of DETs suggested substantial transcriptional reprogramming of diverse cellular processes particularly the biotic stress response pathways in Carrizo citrange roots. Many R genes, transcription factors, and several other genes putatively involved in plant immunity were differentially modulated in citrus roots in response to P. parasitica infection. Analysis reported here lays out a strong foundation for future studies aimed at improving resistance of citrus rootstocks to P. parasitica.

Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group

“…In this study of the transcriptome of StAG cultivar accessions stressed with P. infestans at 48 hai, there were significantly underexpressed genes in the presence of the pathogen, while the secondary metabolite biosynthesis pathways were highly significant for both the underexpressed and overexpressed genes. These results were similar to those observed in a transgenic S. tuberosum transcriptome at 0, 6, and 24 hai in the foliage ( Gao and Bradeen, 2016 ), as well as in transcriptomic research in Citrus stressed with P. parasitica ( Naveed et al., 2019 ). It is important to highlight that there were 103 genes with unknown functions among the 303 DEGs.…”

Dual RNA-seq analysis unveils the molecular interactions between coconut (Cocos nucifera L.) and Phytophthora palmivora, the bud rot pathogen

“…Differential gene expression depends on the hosts' response to infection; hence, it can be presumed that coconut might have a delayed induction of defence machinery. Carrizo citrange roots, too, showed a similar expression pattern in response to P. parasitica infection [49]. DEGs at 12 hpi have been implicated in plants' response to pathogen infections, including those of Phytophthora capsici and Pseudomonas syringae [50], [51], [52].…”

“…4). The role of WRKY in improving the resistance in rice to blast and bacterial blight [81], up-regulation of WRKY in Carrizo citrange following P. parasitica infection [49] and in pepper following P. capsici inoculation [82]reveal its resistance-conferring molecular switch role.…”

Nanoporous Quercetin-Loaded Silicon-Stabilized Hybrid Lipid Nanoparticles Alleviate Salt Stress in Tomato Plants

“…The LRR (leucine-rich repeat) receptor-like protein 13 isoform X1 A0A3Q7HM44 corresponds to Solyc08g016310.2.1, a gene upregulated in tomato after infection with Stemphylium lycopersici . Another LRR receptor-like protein, A0A3Q7F2M2, is annotated as RFK1; an LRR RFK1 gene was upregulated in Carrizo citrange rootstock in response to Phytophthora parasitica . A0A3Q7ILP6 is a glutaredoxin-dependent peroxiredoxin, which is a protein controlling cell redox homeostasis, and it thus plays a role in ensuring that reactive oxygen species (ROS) produced after stress are scavenged …”

“…45 Another LRR receptor-like protein, A0A3Q7F2M2, is annotated as RFK1; an LRR RFK1 gene was upregulated in Carrizo citrange rootstock in response to Phytophthora parasitica. 46 A0A3Q7ILP6 is a glutaredoxin-dependent peroxiredoxin, which is a protein controlling cell redox homeostasis, and it thus plays a role in ensuring that reactive oxygen species (ROS) produced after stress are scavenged. 47 The protein with ID A0A3Q7HWT8 corresponds to a type II phosphoinositide 4-kinase (PIK) γ4 containing an Nterminal UBL (ubiquitin-like) domain.…”