Transcriptome identification and characterization of long non-coding RNAs in the ovary of hens at four stages

Li, Qi; Li, Jing; Li, Chong; Wu, Xing; Si, Sujin; Yang, Pengkun; Li, Wenting; Han, Ruili; Li, Guoxi; Liu, Xiaojun; Kang, Xin; Tian, Yadong

doi:10.1080/10495398.2021.2024217

Cited by 4 publications

(4 citation statements)

References 51 publications

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“…ECM-receptor interaction and focal adhesion are involved in the early development of geese and ducks ovaries ( 48 ). lncRNAs regulate the functioning of chicken ovaries through their interaction with ECM-receptor and focal adhesion pathways ( 55 ). In this study, focal adhesion was found to be an enrichment pathway associated with higher and lesser egg production, which is in line with other research findings ( 56 ).…”

Section: Discussionmentioning

confidence: 99%

Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens

Xiong,

Li,

Wang

et al. 2024

Front. Vet. Sci.

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Poultry broodiness can cause ovarian atresia, which has a detrimental impact on egg production. Non-coding RNAs (ncRNAs) have become one of the most talked-about topics in life sciences because of the increasing evidence of their novel biological roles in regulatory systems. However, the molecular mechanisms of ncRNAs functions and processes in chicken ovarian development remain largely unknown. Whole-transcriptome RNA sequencing of the ovaries of broodiness and laying chickens was thus performed to identify the ncRNA regulatory mechanisms associated with ovarian atresia in chickens. Subsequent analysis revealed that the ovaries of laying chickens and those with broodiness had 40 differentially expressed MicroRNA (miRNAs) (15 up-regulated and 25 down-regulated), 379 differentially expressed Long Noncoding RNA (lncRNAs) (213 up-regulated and 166 down-regulated), and 129 differentially expressed circular RNA (circRNAs) (63 up-regulated and 66 down-regulated). The competing endogenous RNAs (ceRNA) network analysis further revealed the involvement of ECM-receptor interaction, AGE-RAGE signaling pathway, focal adhesion, cytokine-cytokine receptor interaction, inflammatory mediator regulation of TRP channels, renin secretion, gap junction, insulin secretion, serotonergic synapse, and IL-17 signaling pathways in broodiness. Upon further analysis, it became evident that THBS1 and MYLK are significant candidate genes implicated in the regulation of broodiness. The expression of these genes is linked to miR-155-x, miR-211-z, miR-1682-z, gga-miR-155, and gga-miR-1682, as well as to the competitive binding of novel_circ_014674 and MSTRG.3306.4. The findings of this study reveal the existence of a regulatory link between non-coding RNAs and their competing mRNAs, which provide a better comprehension of the ncRNA function and processes in chicken ovarian development.

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Section: Discussionmentioning

confidence: 99%

Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens

Xiong,

Li,

Wang

et al. 2024

Front. Vet. Sci.

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“…However, the female germ cells are arrested for a longer time in meiotic prophase I as they enter into meiosis much earlier than the male germ cells. Several genes and non-coding RNA transcripts are exclusively expressed and/or developmentally regulated in order to play a critical role during oocyte meiosis [ 12 , 13 , 14 ]. For instance, with respect to the above convergent genes, CDC45 plays a critical role in DNA replication to ensure that chromosomal DNA is replicated only once per cell cycle [ 68 ].…”

Section: Chicken Oocyte Expressed Genes Convergent With the Human Oocytementioning

confidence: 99%

“…In addition, the oocytes store several RNA transcripts, proteins, and cytoplasmic organelles that are passed to offspring and play a critical role during zygotic genome activation [ 10 , 11 ]. Moreover, it was reported that a large number of genes are differentially expressed and play a critical role during oocyte meiosis [ 12 , 13 , 14 ]. The development of PGCs, oocytes, and ovarian follicles involves highly conserved processes among mammalian species and non-mammalian vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Female Germ Cell Development in Chickens and Humans: The Chicken Oocyte Enriched Genes Convergent and Divergent with the Human Oocyte

Rengaraj

Han

2022

IJMS

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The development of germ cells and other physiological events in the differentiated ovary of humans are highly conserved with several mammalian species, except for the differences in timing. However, comparative knowledge on this topic is very scarce with respect to humans and lower vertebrates, such as chickens. In chickens, female germ cells enter into meiosis around embryonic day (E) 15.5 and are arrested in meiotic prophase I as primary oocytes. The oocytes arrested in meiosis I are accumulated in germ-cell cysts; shortly after hatching, they are enclosed by flattened granulosa cells in order to form primordial follicles. In humans, the process of meiotic recombination in female germ cells begins in the 10–11th week of gestation, and primordial follicles are formed at around week 20. In this review, we comprehensively elucidate both the conservation and the species-specific differences between chickens and humans with respect to germ cell, oocyte, and follicle development. Importantly, we provide functional insights into a set of chicken oocyte enriched genes (from E16 to 1 week post-hatch) that show convergent and divergent expression patterns with respect to the human oocyte (from week 11 to 26).

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“…Li et al compared differences in the expression profiles of lncRNAs in the uteri of older and younger laying hens and found that 12 lncRNAs ( LNC_013443, LNC_001029 and LNC_005713 , etc.) regulated the deterioration of eggshell quality during the later stages of laying ( Li et al, 2023 ). MiRNAs are a class of ncRNA with a length of 18-25 nucleotides, whose main function is to regulate gene expression by binding to the 3′UTR complementary sequence of target mRNAs ( Kumar et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Whole transcriptome sequencing reveals key genes and ceRNA regulatory networks associated with pimpled eggs in hens

Li,

Cao,

et al. 2024

Poultry Science

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Transcriptome identification and characterization of long non-coding RNAs in the ovary of hens at four stages

Cited by 4 publications

References 51 publications

Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens

Whole transcriptome analysis revealed the regulatory network and related pathways of non-coding RNA regulating ovarian atrophy in broody hens

Female Germ Cell Development in Chickens and Humans: The Chicken Oocyte Enriched Genes Convergent and Divergent with the Human Oocyte

Whole transcriptome sequencing reveals key genes and ceRNA regulatory networks associated with pimpled eggs in hens

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