Transcriptome‐wide isolation and expression of <scp>NF‐Y</scp> gene family in male cone development and hormonal treatment of <scp>Pinus tabuliformis</scp>

Guo, Yang-Dong; Niu, Shihui; El‐Kassaby, Yousry A.; Li, Wei

doi:10.1111/ppl.13183

Cited by 16 publications

(11 citation statements)

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“…WRKY75 could also positively regulate the JA-mediated plant defense against B. cinerea and A. brassicicola by regulating genes such as ORA59 and PDF1.2 in Arabidopsis (Chen et al, 2020). In Pinus tabuliformis, the NF-Ys were shown to respond to SA and JA treatments, which suggests their roles in affecting disease resistance by dynamically regulating SA and JA signaling (Guo et al, 2020). In this study, the PdbNF-Y promoters contain elements related to hormone response (Figure 4), and the protein-protein interaction (PPI) network showed that the PdbNF-Ys may interact with TFs involved in plant hormone signal transduction, such as ERF, MYB, WRKY, and bHLH (Figure 5, Supplementary Table S3).…”

Section: Discussionmentioning

confidence: 99%

“…As a transcription factor (TF) that regulates plant growth and stress resistance, the NF-Y has been identified and studied in multiple plants. There were 36 NF-Ys in A. thaliana (Siefers et al, 2009), 25 NF-Ys in Ricinus communi (Wang et al, 2018), 59 NF-Ys in Solanum lycopersicum , 32 NF-Ys in Vitis vinifera (Ren et al, 2016), 33 NF-Ys in Juglans regia (Quan et al, 2018), 24 NF-Ys in peach (Li et al, 2019), 28 NF-Ys in Pinus tabuliformis (Guo et al, 2020), 52 NF-Ys in P. trichocarpa (Liu et al, 2021), and 46 NF-Ys in P. tomentosa (Li et al, 2022). In this study, a total of 26 NF-Y members were cloned in P. davidiana × P. bollena, including 12 PdbNF-YAs, six PdbNF-YBs, and eight PdbNF-YCs (Table 1, Supplementary Figure S1).…”

Section: Discussionmentioning

confidence: 99%

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Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance

Huang

Ma²,

Wang³

et al. 2022

Front. Bioeng. Biotechnol.

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Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena, including 12 PdbNF-YAs, six PdbNF-YBs, and eight PdbNF-YCs. Their physical and chemical parameters, conserved domains, and phylogeny were subsequently analyzed. The protein–protein interaction (PPI) network showed that the three PdbNF-Y subunits may interact with NF-Y proteins belonging to two other subfamilies and other TFs. Tissue expression analysis revealed that PdbNF-Ys exhibited three distinct expression patterns in three tissues. Cis-elements related to stress-responsiveness were found in the promoters of PdbNF-Ys, and most PdbNF-Ys were shown to be differentially expressed under Alternaria alternata and hormone treatments. Compared with the PdbNF-YB and PdbNF-YC subfamilies, more PdbNF-YAs were significantly induced under the two treatments. Moreover, loss- and gain-of-function analyses showed that PdbNF-YA11 plays a positive role in poplar resistance to A. alternata. Additionally, RT‒qPCR analyses showed that overexpression and silencing PdbNF-YA11 altered the transcript levels of JA-related genes, including LOX, AOS, AOC, COI, JAZ, ORCA, and MYC, suggesting that PdbNF-YA11-mediated disease resistance is related to activation of the JA pathway. Our findings will contribute to functional analysis of NF-Y genes in woody plants, especially their roles in response to biotic stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance

Huang

Ma²,

Wang³

et al. 2022

Front. Bioeng. Biotechnol.

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“…NF‐YB and NF‐YC can form a heterodimer, which then binds to the binding domain on NF‐YA to form heterotrimers (Nardone et al ., 2017). The heterotrimeric transcription factor NF‐Y has been reported to play an important role in plant growth and development and stress response (Mu et al ., 2013; Lian et al ., 2018; Das et al ., 2019; Guo et al ., 2021). As the most important component factor of heterotrimers, the NF‐YA subunit contains two structurally extended α‐helices; one is involved in the NF‐YB/NF‐YC binding domain, and the other is positioned deep into the DNA minor groove, providing exquisite sequence specificity for recognition and binding of the CCAAT box (Nardone et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

miR169o and ZmNF‐YA13 act in concert to coordinate the expression of ZmYUC1 that determines seed size and weight in maize kernels

et al. 2022

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MicroRNAs (miRNAs) play key regulatory roles in seed development and emerge as new key targets for engineering grain size and yield. The Zma-miRNA169 family is highly expressed during maize seed development, but its functional roles in seed development remain elusive.Here, we generated zma-miR169o and ZmNF-YA13 transgenic plants. Phenotypic and genetic analyses were performed on these lines. Seed development and auxins contents were investigated.Overexpression of maize miRNA zma-miR169o increases seed size and weight, whereas the opposite is true when its expression is suppressed. Further studies revealed that zma-miR169 acts by negatively regulating its target gene, a transcription factor ZmNF-YA13 that also plays a key role in determining seed size. We demonstrate that ZmNF-YA13 regulates the expression of the auxin biosynthetic gene ZmYUC1, which modulates auxin levels in the early developing seeds and determines the number of endosperm cells, thereby governing maize seed size and ultimately yield.Overall, our present study has identified zma-miR169o and ZmNF-YA13 that form a functional module regulating auxin accumulation in maize seeds and playing an important role in determining maize seed size and yield, providing a set of novel molecular tools for yield improvement in molecular breeding and genetic engineering.

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“…By contrast, each NF-Y subfamily in plants consists of multiple members. At present, NF-Y family members have been identified in many species, including Arabidopsis [ 12 ], rice [ 13 ], maize [ 14 ], tomato [ 15 ], poplar [ 16 ], and Pinus tabuliformis [ 17 ]. Some species members expanded, resulting in functional redundancy and functional differences, while also helping to form a transcription factor network to regulate plant growth and development [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analyses of Four PtoNF-YC Genes from Populus tomentosa and Impacts on Flowering Timing

Gao

Yang

et al. 2022

IJMS

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Flowering is an important link in the life process of angiosperms, and it is also an important sign of the transformation of plants from vegetative to reproductive growth. Although the flowering regulation network of Arabidopsis is well-understood, there has been little research on the molecular mechanisms of perennial woody plant flower development regulation. Populus tomentosa is a unique Chinese poplar species with fast growth, strong ecological adaptability, and a long lifecycle. However, it has a long juvenile phase, which seriously affects its breeding process. Nuclear factor-Y (NF-Y) is an important type of transcription factor involved in the regulation of plant flowering. However, there are few reports on PtoNF-Y gene flowering regulation, and the members of the PtNF-YC subfamily are unknown. In this study, four key genes were cloned and analyzed for sequence characteristics, gene structure, genetic evolution, expression patterns, and subcellular localization. The plant expression vector was further constructed, and transgenic Arabidopsis and P. tomentosa plants were obtained through genetic transformation and a series of molecular tests. The flowering time and other growth characteristics were analyzed. Finally, the expression level of flowering genes was detected by quantitative PCR, the interaction between PtoNF-YC and PtoCOL proteins was measured using the yeast two-hybrid system to further explain the flowering regulation mechanism, and the molecular mechanisms by which PtNF-YC6 and PtNF-YC8 regulate poplar flowering were discussed. These results lay the foundation for elucidating the molecular regulation mechanism of PtoNF-YC in flowering and furthering the molecular design and breeding of poplar, while providing a reference for other flowering woody plants.

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Transcriptome‐wide isolation and expression of NF‐Y gene family in male cone development and hormonal treatment of Pinus tabuliformis

Cited by 16 publications

References 68 publications

Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance

Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance

miR169o and ZmNF‐YA13 act in concert to coordinate the expression of ZmYUC1 that determines seed size and weight in maize kernels

Comprehensive Analyses of Four PtoNF-YC Genes from Populus tomentosa and Impacts on Flowering Timing

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