Transcriptome datasets of neural progenitors and neurons differentiated from induced pluripotent stem cells of healthy donors and Parkinson's disease patients with mutations in the PARK2 gene

Новосадова, Е. В.; Anufrieva, Ksenia S.; Казанцева, Е. А.; Arsenyeva, E. L.; Fedoseyeva, V.B.; Stepanenko, Ekaterina; Poberezhniy, Daniil; Иллариошкин, С.Н.; Novosadova, Lyudmila; Gerasimova, Tatiana; Nenasheva, Valentina V.; Гривенников, И. А.; Lagarkova, Maria A.; Tarantul, V. Z.

doi:10.1016/j.dib.2022.107958

Cited by 7 publications

(10 citation statements)

References 14 publications

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“…At the same time, samples of healthy donors and donors with PD behave similarly without clusters. All mature neurons from set51 [ 36 ] were located in the area of the neuronal progenitors, as well as some PD neurons from set32 [ 37 ]. Perhaps some differences in the expression profile can be explained by the fact that the meaning of the concept of “mature neurons” is somewhat different for different authors.…”

Section: Resultsmentioning

confidence: 99%

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An Efficient 2D Protocol for Differentiation of iPSCs into Mature Postmitotic Dopaminergic Neurons: Application for Modeling Parkinson’s Disease

Lebedeva

Шарова

Grekhnev

et al. 2023

IJMS

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About 15% of patients with parkinsonism have a hereditary form of Parkinson’s disease (PD). Studies on the early stages of PD pathogenesis are challenging due to the lack of relevant models. The most promising ones are models based on dopaminergic neurons (DAns) differentiated from induced pluripotent stem cells (iPSCs) of patients with hereditary forms of PD. This work describes a highly efficient 2D protocol for obtaining DAns from iPSCs. The protocol is rather simple, comparable in efficiency with previously published protocols, and does not require viral vectors. The resulting neurons have a similar transcriptome profile to previously published data for neurons, and have a high level of maturity marker expression. The proportion of sensitive (SOX6+) DAns in the population calculated from the level of gene expression is higher than resistant (CALB+) DAns. Electrophysiological studies of the DAns confirmed their voltage sensitivity and showed that a mutation in the PARK8 gene is associated with enhanced store-operated calcium entry. The study of high-purity DAns differentiated from the iPSCs of patients with hereditary PD using this differentiation protocol will allow for investigators to combine various research methods, from patch clamp to omics technologies, and maximize information about cell function in normal and pathological conditions.

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Section: Resultsmentioning

confidence: 99%

“…According to the expression signature, mature neurons were produced by all protocols except set51 [ 36 ]. Set32 [ 37 ] also demonstrates maturity despite its location on the PCA. The DAn signature is not consistent among the datasets.…”

Section: Resultsmentioning

confidence: 99%

An Efficient 2D Protocol for Differentiation of iPSCs into Mature Postmitotic Dopaminergic Neurons: Application for Modeling Parkinson’s Disease

Lebedeva

Шарова

Grekhnev

et al. 2023

IJMS

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“…In an experiment, neural progenitors and terminally differentiated neurons were derived from iPSCs lines from three healthy donors and three PD patients. At the end, a comparative transcriptome analysis of all patients was conducted to demonstrate how PARK2 can contribute to the pathogenesis (29).…”

Section: Parkinson's Diseasementioning

confidence: 99%

Stem Cells and their Extensive uses in Multiple Medical Fields and Especially in Neurological Diseases

Sarnataro¹

2022

ABCMed

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Since they were first discovered and used in animal models in the 1950th, stem cells are assuming a key role in the research field. These types of cells are undifferentiated cells deriving from a single cell that is able of a clonal proliferation differentiating into different cells and tissues. It is possible to classify them into two main groups considering their main features. Moreover, speaking about stem cells, it is important to mention the key role of some small non-coding RNA molecules also known MiRNAs (microRNAs) in the cell cycle regulation. Generally, stem cells are very useful in cellular therapy helping the regeneration of entire organs. They also allow a deep understanding of development and pathogenesis of many diseases and are useful in drug development. In general, the aim of this article is to talk about stem cells and their uses in multiple medical fields and especially in neurological diseases. A comprehensive explanation of stem cell classification and miRNAs role in the cell cycle regulation of these cells in clinical application as well as gene alterations in some diseases is included. And finally, very interesting stem cell research will be also discussed. Unfortunately, studies on adhesive arachnoiditis, a rare neurological disease mostly associated to Tarlov cysts (TC), are still few. In addition, other associations between arachnoiditis and Chiari malformation, as well as syringomyelia and ACM, have been found. And, finally, association between idiopathic intracerebral hypertension and arachnoiditis granulations (AGs) were also demonstrated.

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“…Studying dysregulated genes in DA neurons is crucial for deepening our understanding of the molecular mechanisms underlying PD. Using powerful tools like high throughput RNA sequencing, attempts have been made to identify dysregulated genes in the disease state by comparing gene expression patterns in healthy versus PD-afflicted DA neurons [4][5][6][7][8][9][10] . Analyzing the postmortem brain tissue from PD patients provides a direct view of gene expression patterns in affected DA neurons [4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

“…This approach offers a unique opportunity to study gene expression patterns in DA neurons specific to PD patients. CRISPR/Cas9 and other gene editing technologies have also enabled researchers to introduce or correct gene mutations associated with PD in cellular or animal models to investigate the direct impact of specific gene dysregulation on DA neuron function and survival 5 . Despite the differences in their genetic basis, some common pathways and mechanisms may contribute to the degeneration of DA neurons in both familial and sporadic PD.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the genes and pathways that are dysregulated in dopaminergic neurons during both familial and sporadic Parkinson’s disease

Padhan,

Verma

2024

Preprint

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Identification of shared dysregulated genes and molecular mechanisms responsible for dopaminergic (DA) neuronal death in familial and sporadic forms of Parkinson’s disease (PD) can offer the potential for common therapies in both types of PD. The gene expression profiles of DA neurons with sporadic and familial PD backgrounds and healthy DA neurons were obtained from the Gene Expression Omnibus database. Overlapping differentially expressed genes and hub genes were identified. These genes were subjected to Gene Ontology and Reactome pathway enrichment analyses. The roles of a select few differentially expressed genes inC. elegansDA neuron health were assessed. 40 genes, including 12 hub genes, were found dysregulated in DA neurons with sporadic and familial PD. Altering the expression of some of these genes in wild-typeCaenorhabditis eleganspromoted DA neuron degeneration and function. The study unveils shared molecular mechanisms in DA neurons for familial and sporadic PD, potentially driving DA neuron degeneration. Understanding their roles may lead to targeted therapies, early detection, and intervention.In vivoexperiments support our hypothesis, suggesting the implication of these genes in PD neurodegeneration and neuroprotection.

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Transcriptome datasets of neural progenitors and neurons differentiated from induced pluripotent stem cells of healthy donors and Parkinson's disease patients with mutations in the PARK2 gene

Cited by 7 publications

References 14 publications

An Efficient 2D Protocol for Differentiation of iPSCs into Mature Postmitotic Dopaminergic Neurons: Application for Modeling Parkinson’s Disease

An Efficient 2D Protocol for Differentiation of iPSCs into Mature Postmitotic Dopaminergic Neurons: Application for Modeling Parkinson’s Disease

Stem Cells and their Extensive uses in Multiple Medical Fields and Especially in Neurological Diseases

Unveiling the genes and pathways that are dysregulated in dopaminergic neurons during both familial and sporadic Parkinson’s disease

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