Transcriptome datasets of macrophages infected with different strains of Leptospira spp

Abstract

The datasets reported herein provide information about microarray experiment of macrophage cell line J774A.1 infected with three different strains of Leptospira spp. Transcriptomic profiles were generated using Affymetrix® Mouse Gene 2.1 ST Array Strip. Data was normalized and statically process, p-value < 0.01, FDR < 0.05 and log2 fold change (± 2). The microarray raw data are available in Gene Expression Omnibus (GEO) under accession number GSE105141.

Gene expression is associated with virulence in murine macrophages infected with Leptospira spp

“…Microarray raw data files are available in Gene Expression Omnibus (GEO) and are accessible through GEO series number GSE105141 [22].…”

“…Murine monocyte-macrophage cells ( Mus musculus monocyte-macrophage cell line J774A.1), provided by the Paul Ehrlich cell bank (Rio de Janeiro, Brazil), was used as described [26]. Cells were maintained at 37° C, 5% CO2 in RPMI-1640 media (Sigma, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco, USA), 100 ug/mL streptomycin (Sigma Chemical Co St.Louis, MO), 0.03% L-glutamine solution (Sigma) and 100 UI/mL of penicillin, in 6-well cell culture plates (3cm/well) until confluency [22,29].…”

“…The quantification was performed using a NanoDrop (ND-2000 spectrophotometer, Thermo Scientific, Wilmington, DE, USA) and the samples quality was assessed using capillary electrophoresis (Bioanalyzer 2100 Agilent, Santa Clara, CA, USA). All samples used for microarray analysis had a RIN of 10 (Quality data is available on our data descriptor)[22].…”

Gene expression is associated with virulence in murine macrophages infected withLeptospiraspp

“…Microarray raw data files are available in Gene Expression Omnibus (GEO) and are accessible through GEO series number GSE105141 [26].…”

“…Murine monocyte-macrophage cells ( Mus musculus monocyte-macrophage cell line J774A.1), provided by the Paul Ehrlich cell bank (Rio de Janeiro, Brazil), was used as described [26]. Cells were maintained at 37º C, 5% CO2 in RPMI-1640 media (Sigma, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco, USA), 100 ug/mL streptomycin (Sigma Chemical Co St.Louis, MO), 0.03% L-glutamine solution (Sigma) and 100 UI/mL of penicillin, in 6-well cell culture plates (3cm/well) until confluency [35].…”