Transcriptome Dataset of Soybean (Glycine max) Grown under Phosphorus-Deficient and -Sufficient Conditions

Abstract

This data descriptor introduces the dataset of the transcriptome of low-phosphorus tolerant soybean (Glycine max) variety NN94-156 under phosphorus-deficient and -sufficient conditions. This data is comprised of the transcriptome datasets (four libraries) acquired from roots and leaves of the soybean plants challenged with low-phosphorus, which allows further analysis whether systemic tolerance response to low phosphorus stress occurred. We describe the detailed procedure of how plants were prepared and treated and how the data were generated and pre-processed. Further analyses of this data would be helpful to improve our understanding of molecular mechanisms of low-phosphorus stress in soybean.Data Set: https://www.ncbi.nlm.nih.gov/sra/?term=SRP097715 Data Set License: There is no specific license.

Physiological and Proteomics Analyses Reveal Low-Phosphorus Stress Affected the Regulation of Photosynthesis in Soybean

“…Our previous RNA-Seq studies have shown that many genes involved in photosynthesis showed a significant change in the expression during low-P treatment in B20 [ 22 ]. These differentially-expressed genes (DEGs) were found to be mainly involved in photosynthesis, Calvin cycle, light reaction, carbon metabolism, photoreceptor, and photoprotection.…”

Legume genetic resources and transcriptome dynamics under abiotic stress conditions

“…NGS technologies have now been applied to many important crop plants to help understand the genetics of complex traits, and to provide insight into genomic variations, such as single nucleotide polymorphisms (SNPs), insertions/deletions, and other structural of variances (O'Rourke, Bolon, Bucciarelli, & Vance, ; Valliyodan et al, ; Yuan, Bayer, Batley, & Edwards, ). These advanced technologies have also facilitated the development of gene expression atlases and increased our understanding of the signalling pathways involved in the responses of plants to environmental stresses (Abdelrahman et al, ; Abdelrahman, Suzumura, et al, ; Abdelrahman, El‐Sayed, Sato, et al, ; Bhattacharjee, Ghangal, Garg, & Jain, ; Nasr Esfahani et al, ; Zhang, Chu, & Zhang, ). However, during domestication, the genomes of many crop species have changed relative to that of their wild progenitors, with increasing numbers of repetitive DNA sequences that can lead to incomplete or misassembled regions when NGS is used for sequencing, as NGS‐read lengths are too short to adequately cover these repeats (Abdelrahman, Suzumura, et al, ; Flint‐Garcia, ; Pavlovich, ; Yuan et al, ).…”

“…In addition, a shift in cultivated land use toward legume production can potentially lower the carbon footprint for the production of proteins needed for human consumption, increase N use efficiency, and enhance soil fertility (Considine et al, ; Foyer et al, ; Song et al, ; Sosa‐Valencia et al, ). However, the sustainability of legume crop production is threatened by the increasing incidence of environmental stresses, including persistent drought, increasing soil salinity, heat waves, and soil nutritional deficiencies (Aranjuelo, Cabrerizo, Aparicio‐Tejo, & Arrese‐Igor, ; Kunert et al, ; Maqbool, Aslam, & Ali, ; Nasr Esfahani et al, ; Valdés‐López et al, ; Zhang et al, ). For these reasons, it is essential to intensify legume genetic enrichment programmes, by using advanced breeding approaches and techniques, to develop legume cultivars that possess genetic resilience to environmental stresses.…”

“…The family Fabaceae (or Leguminosae) consists of approximately 18,000 species within approximately 700 genera with many of these species, including soybean (Glycine max), chickpea (Cicer arietinum), groundnut (Arachis hypogaea), common bean (Phaseolus vulgaris), garden pea (Pisum sativum), pigeon pea (Cajanus cajan), and alfalfa (Medicago sativa), being important dietary components for humans and farm animals (Cao, Takeshima, et al, 2017;Cao, Li, et al, 2017;Considine, Siddique, & Foyer, 2017;Foyer et al, 2016;Song et al, 2016;Sosa-Valencia, Palomar, Covarrubias, & Reyes, 2017;Valliyodan et al, 2016). At present, 33% of the dietary nitrogen (N) requirement for humans is obtained from legumes, and 40% of the world's cooking oil is derived from soybean and groundnut (O'Rourke et al, 2014;Song et al, 2016;Zhang et al, 2017). Therefore, legumes play a vital role in maintaining global food security (Ainsworth, Yendrek, Skoneczka, & Long, 2012;Araújo et al, 2015;Nasr Esfahani et al, 2017;Pandey et al, 2016).…”

“…However, the sustainability of legume crop production is threatened by the increasing incidence of environmental stresses, including persistent drought, increasing soil salinity, heat waves, and soil nutritional deficiencies (Aranjuelo, Cabrerizo, Aparicio-Tejo, & Arrese-Igor, 2014;Kunert et al, 2016;Maqbool, Aslam, & Ali, 2017;Nasr Esfahani et al, 2017;Valdés-López et al, 2016;Zhang et al, 2017). For these reasons, it is essential to intensify legume genetic enrichment programmes, by using advanced breeding approaches and techniques, to develop legume cultivars that possess genetic resilience to environmental stresses.…”