Transcriptome data analysis of grass carp ( Ctenopharyngodon idella ) infected by reovirus provides insights into two immune-related genes

Dai, Zao; Li, Jicheng; Hu, Chengyu; Wang, Fang; Wang, Binhua; Shi, Xin; Hou, Qunhao; Huang, Waigen; Lin, Gang

doi:10.1016/j.fsi.2017.03.008

Cited by 26 publications

(8 citation statements)

References 53 publications

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“…The IgM expression levels were higher in the liver, spleen, and kidney in the VP35-VP4 group than in the other two groups. Previous studies have shown a significant increase in the expression of IgM after GCRV infection in the rare minnow, and the "Influenza A pathway" plays a critical role in the defense mechanisms against GCRV in the rare minnow; this is consistent with the results reported in grass carp [16,51]. Ou et al (2019) showed that the expression of GrSRB1 in the main immune tissues of the rare minnow was upregulated after GCRV infection; this suggests that GrSRB1 is associated with GCRV and could bring GCRV to the cells, which facilitates virus attachment and entry [52].…”

Section: Discussionsupporting

confidence: 90%

A Novel Subunit Vaccine Based on Outer Capsid Proteins of Grass Carp Reovirus (GCRV) Provides Protective Immunity against GCRV Infection in Rare Minnow (Gobiocypris rarus)

Vakharia

Zhou

et al. 2020

Pathogens

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The grass carp hemorrhagic disease, caused by the grass carp reovirus (GCRV), has resulted in severe economic losses in the aquaculture industry in China. VP4 and VP35 are outer capsid proteins of GCRV and can induce an immune response in the host. Here, three recombinant baculoviruses, AcMNPV-VP35, AcMNPV-VP4, and AcMNPV-VP35-VP4, were generated to express recombinant VP4 and VP35 proteins from GCRV type II in insect cells by using the Bac-to-Bac baculovirus expression system to create a novel subunit vaccine. The expression of recombinant VP35, VP4, and VP35-VP4 proteins in Sf-9 cells were confirmed by Western blotting and immunofluorescence. Recombinant VP35, VP4, and VP35-VP4 were purified from baculovirus-infected cell lysates and injected intraperitoneally (3 μg/fish) into the model rare minnow, Gobiocypris rarus. After 21 days, the immunized fish were challenged with virulent GCRV. Liver, spleen, and kidney samples were collected at different time intervals to evaluate the protective efficacy of the subunit vaccines. The mRNA expression levels of some immune-related genes detected by using quantitative real-time PCR (qRT-PCR) were significantly upregulated in the liver, spleen, and kidney, with higher expression levels in the VP35-VP4 group. The nonvaccinated fish group showed 100% mortality, whereas the VP35-VP4, VP4, and VP35 groups exhibited 67%, 60%, and 33% survival, respectively. In conclusion, our results revealed that recombinant VP35 and VP4 can induce immunity and protect against GCRV infection, with their combined use providing the best effect. Therefore, VP35 and VP4 proteins can be used as a novel subunit vaccine against GCRV infection.

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Section: Discussionsupporting

confidence: 90%

A Novel Subunit Vaccine Based on Outer Capsid Proteins of Grass Carp Reovirus (GCRV) Provides Protective Immunity against GCRV Infection in Rare Minnow (Gobiocypris rarus)

Vakharia

Zhou

et al. 2020

Pathogens

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“…The most induced genes after NNV infection with the wt virus were VRGs, particularly RTP3, SACS, and TRIM39 [6]. Since betanodaviruses have been proven to cause cell death via mitochondrial targeting, Sacsin could be counteracting the NNV-induced cellular apoptosis [77]. RTP3 plays a role in the Asian sea bass resistance against NNV, and the direct anti-NNV activity of grouper TRIM39 has been demonstrated in over-expression experiments [78,79].…”

Section: Senegalese Sole (Solea Senegalensis)mentioning

confidence: 99%

Transcriptomic Analysis of Fish Hosts Responses to Nervous Necrosis Virus

2022

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Nervous necrosis virus (NNV) has been responsible for mass mortalities in the aquaculture industry worldwide, with great economic and environmental impact. The present review aims to summarize the current knowledge of gene expression responses to nervous necrosis virus infection in different fish species based on transcriptomic analysis data. Four electronic databases, including PubMed, Web of Science, and SCOPUS were searched, and more than 500 publications on the subject were identified. Following the application of the appropriate testing, a total of 24 articles proved eligible for this review. NNV infection of different host species, in different developmental stages and tissues, presented in the eligible publications, are described in detail, revealing and highlighting genes and pathways that are most affected by the viral infection. Those transcriptome studies of NNV infected fish are oriented in elucidating the roles of genes/biomarkers for functions of special interest, depending on each study’s specific emphasis. This review presents a first attempt to provide an overview of universal host reaction mechanisms to viral infections, which will provide us with new perspectives to overcome NNV infection to build healthier and sustainable aquaculture systems.

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“…Similarly, previous RNA-Seq data from the grass carp during GCRV infection was used in order to check the performance of two downstream bioinformatics tools, i.e., DESeq and Cufflinks, which were used to calculate the differential expression of genes during RNA-Seq data analysis. This study provided insights into two immune-related genes, ISG15 an ubiquitin-like protein and sacsin-like gene which is likely to regulate mitochondrial dynamics in the grass carp [ 105 ]. It is clear that RNA-Seq-based transcriptomics analysis is an inevitable tool not only to unveil host–viral interactions in aquatic organisms, but also to help to characterize the viral mechanisms to escape the host defense mechanisms.…”

Section: Fish Immune Response To Viral Pathogensmentioning

confidence: 99%

Transcriptome Analysis Based on RNA-Seq in Understanding Pathogenic Mechanisms of Diseases and the Immune System of Fish: A Comprehensive Review

Sudhagar

Kumar

El-Matbouli

2018

IJMS

121

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In recent years, with the advent of next-generation sequencing along with the development of various bioinformatics tools, RNA sequencing (RNA-Seq)-based transcriptome analysis has become much more affordable in the field of biological research. This technique has even opened up avenues to explore the transcriptome of non-model organisms for which a reference genome is not available. This has made fish health researchers march towards this technology to understand pathogenic processes and immune reactions in fish during the event of infection. Recent studies using this technology have altered and updated the previous understanding of many diseases in fish. RNA-Seq has been employed in the understanding of fish pathogens like bacteria, virus, parasites, and oomycetes. Also, it has been helpful in unraveling the immune mechanisms in fish. Additionally, RNA-Seq technology has made its way for future works, such as genetic linkage mapping, quantitative trait analysis, disease-resistant strain or broodstock selection, and the development of effective vaccines and therapies. Until now, there are no reviews that comprehensively summarize the studies which made use of RNA-Seq to explore the mechanisms of infection of pathogens and the defense strategies of fish hosts. This review aims to summarize the contemporary understanding and findings with regard to infectious pathogens and the immune system of fish that have been achieved through RNA-Seq technology.

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Transcriptome data analysis of grass carp ( Ctenopharyngodon idella ) infected by reovirus provides insights into two immune-related genes

Cited by 26 publications

References 53 publications

A Novel Subunit Vaccine Based on Outer Capsid Proteins of Grass Carp Reovirus (GCRV) Provides Protective Immunity against GCRV Infection in Rare Minnow (Gobiocypris rarus)

A Novel Subunit Vaccine Based on Outer Capsid Proteins of Grass Carp Reovirus (GCRV) Provides Protective Immunity against GCRV Infection in Rare Minnow (Gobiocypris rarus)

Transcriptomic Analysis of Fish Hosts Responses to Nervous Necrosis Virus

Transcriptome Analysis Based on RNA-Seq in Understanding Pathogenic Mechanisms of Diseases and the Immune System of Fish: A Comprehensive Review

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