Transcriptome Characterization of the Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook.) and Expression Analysis of Candidate Phosphate Transporter Genes

Li, Ming; Su, Suo-Suo; Wu, Pengfei; Cameron, Kenneth M.; Zhang, Ying; Chen, Wanting; Ma, Xiao

doi:10.3390/f8110420

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…To screen for genes that are constitutively expressed in Chinese fir, the expression profiling was performed using the public the transcriptomic data from public database was downloaded from NCBI (SRX2586190, SRX2586189, SRX2586188, and SRX139598) ( Li et al., 2017 ; Wang et al., 2020 ). These analyses identified 24 genes that were expressed in all tissues at high levels ( Supplemental Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Application of a novel strong promoter from Chinese fir (Cunninghamia lanceolate) in the CRISPR/Cas mediated genome editing of its protoplasts and transgenesis of rice and poplar

Ding

Bai

et al. 2023

Front. Plant Sci.

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Novel constitutive promoters are essential for plant biotechnology. Although in angiosperms, a number of promoters were applied in monocots or dicots genetic engineering, only a few promoters were used in gymnosperm. Here we identified two strong promoters (Cula11 and Cula08) from Chinese fir (C. lanceolate) by screening the transcriptomic data and preliminary promoter activity assays in tobacco. By using the newly established Chinese fir protoplast transient expression technology that enables in vivo molecular biology studies in its homologous system, we compared the activities of Cula11 and Cula08 with that of the commonly used promoters in genetic engineering of monocots or dicots, such as CaM35S, CmYLCV, and ZmUbi, and our results revealed that Cula11 and Cula08 promoters have stronger activities in Chinese fir protoplasts. Furthermore, the vector containing Cas gene driven by Cula11 promoter and sgRNA driven by the newly isolated CulaU6b polyIII promoters were introduced into Chinese fir protoplasts, and CRISPR/Cas mediated gene knock-out event was successfully achieved. More importantly, compared with the commonly used promoters in the genetic engineering in angiosperms, Cula11 promoter has much stronger activity than CaM35S promoter in transgenic poplar, and ZmUbi promoter in transgenic rice, respectively, indicating its potential application in poplar and rice genetic engineering. Overall, the novel putative constitutive gene promoters reported here will have great potential application in gymnosperm and angiosperm biotechnology, and the transient gene expression system established here will serve as a useful tool for the molecular and genetic analyses of Chinese fir genes.

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Section: Resultsmentioning

confidence: 99%

Application of a novel strong promoter from Chinese fir (Cunninghamia lanceolate) in the CRISPR/Cas mediated genome editing of its protoplasts and transgenesis of rice and poplar

Ding

Bai

et al. 2023

Front. Plant Sci.

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“…Physiologically, Chinese fir can respond to Pi starvation with exogenous ethylene to improve the tolerance of seedlings to Pi deficiency [ 17 ]. At the transcriptome level, there have been partial articles on RNA-seq for P stress in Chinese fir, but RNA-seq from the perspective of studying P response from the application of exogenous ethylene has not been implemented [ 7 , 28 , 29 ]. We mined Chinese fir with more consistent growth by RNA-seq for differential genes and transcription factors in Chinese fir regarding the co-interaction of Pi and ethylene.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir

Huang

Zhang

Cai

et al. 2022

Plants

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Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is a widely grown gymnosperm in China. Phosphorus (P) is an indispensable nutrient for the growth of Chinese fir. Inorganic phosphate (Pi) deficiency exists in soils of many Chinese fir planting area regions, and the trees themselves have limited efficiency in utilizing P from the soil. Ethylene is important in regulation responses to nutrient deficiencies. However, little is known about how ethylene signals participate in Pi stress in Chinese fir. A total of six different treatments were performed to reveal the transcript levels of Chinese fir under Pi, ethephon (an ethylene-releasing compound), and CoCl2 (cobalt chloride, an ethylene biosynthesis inhibitor) treatments. We assembled a full-length reference transcriptome containing 22,243 unigenes as a reference for UMI RNA-seq (Digital RNA-seq). There were 586 Differentially Expressed Genes (DEGs) in the Pi starvation (NP) group, while DEGs from additional ethephon or CoCl2 in NP were 708 and 292, respectively. Among the DEGs in each treatment, there were 83 TFs in these treatment groups. MYB (v-myb avian myeloblastosis viral oncogene homolog) family was the most abundant transcription factors (TFs). Three ERF (Ethylene response factor) family genes were identified when only ethylene content was imposed as a variable. Enrichment analysis indicated that the ascorbate and aldarate metabolism pathway plays a key role in resistance to Pi deficiency. This study provides insights for further elucidating the regulatory mechanism of Pi deficiency in Chinese fir.

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“…Wang et al 2007). By the end of the twentieth century, the plantation forests of Chinese fir covered more than 9.11 million ha in China, where it amounted to about 240 million m 3 of forest stock volume and provided approximately 20-25% of the commercial wood used in the entire country (Lei 2005; M. Li and Ritchie 1999).…”

Section: Introductionmentioning

confidence: 99%

Wood Analysis of An Outer Coffin Unearthed From the Qian Zhang Tomb (Ad 1535) In Wuxi of East China

Zhang¹,

Mai²,

Liu³

et al. 2016

Journal of Ethnobiology

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A well-preserved outer coffin, several hundred years old, was discovered under waterlogged conditions in the ancient Qian Zhang Tomb located within Wuxi, an old eastern Chinese city in southern part of Jiangsu Province. Wood anatomy was employed to identify the tree species used to build this ancient outer coffin. The results showed it was constructed of sturdy Chinese fir (Cunninghamia lanceolata). In addition, gas chromatography–mass spectrometry (GC-MS) was undertaken to examine the chemical composition of wood extract. The analysis indicates that cedrol, a sesquiterpene alcohol found in the essential oils, was the major compound of the extract and apparently a key factor responsible for the outstanding durability of this wood. A review of Chinese fir uses recorded in ancient Chinese literature is presented for historical context, along with a list of known archaeological sites that have contained remains of this conifer species in China. A hypothetical explanation for its selection as a coffin construction material by the noble family of Qian Zhang is also discussed.

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Transcriptome Characterization of the Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook.) and Expression Analysis of Candidate Phosphate Transporter Genes

Cited by 7 publications

References 62 publications

Application of a novel strong promoter from Chinese fir (Cunninghamia lanceolate) in the CRISPR/Cas mediated genome editing of its protoplasts and transgenesis of rice and poplar

Application of a novel strong promoter from Chinese fir (Cunninghamia lanceolate) in the CRISPR/Cas mediated genome editing of its protoplasts and transgenesis of rice and poplar

Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir

Wood Analysis of An Outer Coffin Unearthed From the Qian Zhang Tomb (Ad 1535) In Wuxi of East China

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