Transcriptome characterization of candidate genes for heat tolerance in perennial ryegrass after exogenous methyl Jasmonate application

Nie, Gang; Zhou, Jie; Jiang, Yiwei; He, Jie; Wang, Yang; Liao, Zongchao; Appiah, Charlotte; Li, Dandan; Feng, Guangyan; Huang, Linkai; Wang, Xia; Zhang, Xinquan

doi:10.1186/s12870-021-03412-9

Cited by 17 publications

(26 citation statements)

References 69 publications

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“…Exogenous MeJA have been proved to have ROS-scavenging and membrane-protecting properties in various plant species, which can enhance thermotolerance by mediating the expression of genes in the antioxidant enzyme system [50,51]. Our results showed exogenous MeJA could maintain the higher activities of antioxidant enzymes under hightemperature stress, and this process could be accompanied with signaling cascades that start with signal perception and result in a variety of stress responses [52]. Carotenoids (Car) are a type of chlorophyll and an antioxidant substance, and high temperatures induced the increase in the Car content of rice leaves.…”

Section: Effects Of Exogenous Meja At Booting Stage On Rice Yield And...mentioning

confidence: 65%

Exogenous Application of Methyl Jasmonate at the Booting Stage Improves Rice’s Heat Tolerance by Enhancing Antioxidant and Photosynthetic Activities

et al. 2022

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With the intensification of global warming, high temperatures during rice’s growth and development could further lead to a deterioration in rice yields. Therefore, it is particularly important to further clarify the response of the rice booting stage to high temperatures, and to explore reasonable countermeasures on this basis to reduce yield losses. Methyl jasmonate (MeJA) is a derivative of jasmonates and is widely used for stress resistance. However, the role of MeJA in alleviating high temperatures during the rice booting stage has not been given enough attention. This study aimed to further evaluate the alleviation effect of methyl jasmonate on high-temperature stress during the key growth period of local conventional japonica rice. The results showed that high temperatures (37.5 °C/27.0 °C) at the booting stage had a significant impact on the antioxidant system of rice and also significantly reduced the photosynthetic capacity of the plant, resulting in a decrease in the final yields. The exogenous spraying of 0.1 mmol/L MeJA at the booting stage could effectively alleviate the influence of high-temperature stress on rice photosynthesis. Exogenous MeJA increased the stomatal conductance (Gs) of rice leaves under high-temperature stress, and correspondingly increased the transpiration rate (Tr) and decreased the organ temperature of rice plants, thereby reducing the damage to the actual photochemical efficiency (ΦPSII) caused by high temperatures. By increasing the carotenoid content (Car) and reducing the malondialdehyde content (MDA), the antioxidant capacity of the plants was restored to a certain extent under exogenous MeJA, and the yield factor showed an increase in the number of grains per panicle and the seed-setting rate of Wuyunjing 24, which alleviated the booting stage yield losses induced by high-temperature stress. In conclusion, the application of exogenous MeJA at the booting stage alleviated the negative consequences of high temperatures by enhancing the plants’ antioxidant and photosynthetic capacity. Therefore, MeJA may have a potential role in mitigating the challenges of global warming in rice production.

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Section: Effects Of Exogenous Meja At Booting Stage On Rice Yield And...mentioning

confidence: 65%

Exogenous Application of Methyl Jasmonate at the Booting Stage Improves Rice’s Heat Tolerance by Enhancing Antioxidant and Photosynthetic Activities

et al. 2022

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“…Similarly, MeJA protected the photosystem system (PS) II in heat-stressed wheat plants by stabilizing the chloroplast D1 protein and increasing the rate of enzymatic antioxidants 10 . MeJA promoted heat tolerance in perennial ryegrass by altering chlorophyll (Chl) production and degradation, the antioxidant enzyme system, the HSF-HSP network, and jasmonic acid (JA) production 11 . It has been shown that exogenous MeJA promoted the accumulation of JA and MeJA content under heat stress by elevation of the transcript levels of genes involved in the JA biosynthesis pathway 11 , 12 .…”

Section: Introductionmentioning

confidence: 99%

“…MeJA promoted heat tolerance in perennial ryegrass by altering chlorophyll (Chl) production and degradation, the antioxidant enzyme system, the HSF-HSP network, and jasmonic acid (JA) production 11 . It has been shown that exogenous MeJA promoted the accumulation of JA and MeJA content under heat stress by elevation of the transcript levels of genes involved in the JA biosynthesis pathway 11 , 12 . MT also protected tomato seedlings from heat-induced damage by regulating polyamine and nitric oxide production and restoring redox homeostasis 13 .…”

Section: Introductionmentioning

confidence: 99%

“…The study of Siddique et al 17 established that MT and S had a synergistic role in mitigating the negative effect of lanthanum toxicity by upregulating the enzymatic antioxidant and mitigating ROS-induced oxidative damage. The studies suggest that there is a strong connection between MeJA and S-metabolism, and through signaling crosstalk with S-assimilation, MeJA can control a variety of plant responses under stressful and optimal situations 11 , 11 .…”

Section: Introductionmentioning

confidence: 99%

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Melatonin influences methyl jasmonate-induced protection of photosynthetic activity in wheat plants against heat stress by regulating ethylene-synthesis genes and antioxidant metabolism

Sehar

Fatma

Khan

et al. 2023

Sci Rep

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Melatonin (MT) and methyl jasmonate (MeJA) play important roles in the adaptation of plants to different stress factors by modulating stress tolerance mechanisms. The present study reports the involvement of MT (100 µM) in MeJA (10 µM)-induced photosynthetic performance and heat stress acclimation through regulation of the antioxidant metabolism and ethylene production in wheat (Triticum aestivum L.) plants. Plants exposed to 40 °C for 6 h per day for 15 days and allowed to retrieve at 28 °C showed enhanced oxidative stress and antioxidant metabolism, increased 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) activity and ethylene production, and decreased photosynthetic performance. In contrast, the exogenously applied MT and MeJA reduced oxidative stress through improved S-assimilation (+ 73.6% S content), antioxidant defense system (+ 70.9% SOD, + 115.8% APX and + 104.2% GR, and + 49.5% GSH), optimized ethylene level to 58.4% resulting in improved photosynthesis by 75%. The use of p-chlorophenyl alanine, a MT biosynthesis inhibitor along with MeJA in the presence of heat stress reduced the photosynthetic performance, ATP-S activity and GSH content, substantiated the requirement of MT in the MeJA-induced photosynthetic response of plants under heat stress. These findings suggest that MeJA evoked the plant’s ability to withstand heat stress by regulating the S-assimilation, antioxidant defense system, and ethylene production, and improving photosynthetic performance was dependent on MT.

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“…Currently, stable genetic transformation of ryegrass takes at least seven months starting from callus initiation to regeneration of putative transgenic plants (Grogg et al 2022). With the accumulated information of potential heatresponsive genes or genetic loci using comparative transcriptome analysis or Genome-Wide Association Studies (GWAS) (Nie et al 2022;Zhang et al 2020), an e cient and time-saving method is in need to rapidly pinpoint out candidate regulatory genes in ryegrass heat tolerance.…”

Section: Introductionmentioning

confidence: 99%

PRIDA, a protoplast-based assay for efficient identification of oxidative- and heat-stress regulatory genes

Lei

Ling-shuang

Jia

et al. 2023

Preprint

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Background: Rapidly identifying candidate genes potentially involved in stress tolerance is crucial for functional genetic studies in non-model plant species. Agrobacterium-based transient assays can be conducted in many plants using fleshy tissues such as leaf/fruit/petal. However, such assays are not feasible in perennial ryegrass (Lolium perenne L.) and most other grass species since these plants have no fleshy tissue to be readily infected. Results: In this study, we developed a Protoplast-based Rapid stress regulatory gene Identification Assay (briefed as ‘PRIDA’) to rapidly identify genes in the regulation of plant heat and oxidative tolerance in perennial ryegrass. Using ryegrass mesophyll protoplasts, vectors harboring target genes or their RNAi hairpin structures driven under the maize ubiquitin promoter were transformed into protoplasts to transiently over-express or suppress the target genes. The transformed protoplasts were then subjected to optimized heat (35 to 37 ℃) or oxidative (50 to 75 mM H2O2) stress, and their viabilities were immediately recorded under a microscope after 0.1% Evans blue dye staining. The validity and reliability of PRIDA were verified by transiently knocking-down LpNOL/LpSGR and over-expressing LpTT3.1/LpTT3.2 that led to significantly compromised or enhanced protoplast viability after heat or H2O2-induced oxidative stresses. Finally, using ‘PRIDA’, we identified three heat-induced differentially expressed E3 ligase genes as potential regulators in plant heat and/or oxidative stress tolerance. Conclusion: This protoplast-based ‘PRIDA’ can be used as a versatile assay to rapidly identify and characterize candidate regulatory genes in heat and oxidative stress that will accelerate molecular genetic studies in perennial ryegrass. This assay can be adapted to other non-model plant species after adjusting abiotic stress parameters for further research and the development of improved cultivars.

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Transcriptome characterization of candidate genes for heat tolerance in perennial ryegrass after exogenous methyl Jasmonate application

Cited by 17 publications

References 69 publications

Exogenous Application of Methyl Jasmonate at the Booting Stage Improves Rice’s Heat Tolerance by Enhancing Antioxidant and Photosynthetic Activities

Exogenous Application of Methyl Jasmonate at the Booting Stage Improves Rice’s Heat Tolerance by Enhancing Antioxidant and Photosynthetic Activities

Melatonin influences methyl jasmonate-induced protection of photosynthetic activity in wheat plants against heat stress by regulating ethylene-synthesis genes and antioxidant metabolism

PRIDA, a protoplast-based assay for efficient identification of oxidative- and heat-stress regulatory genes

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