Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes

Vigone, Giulia; Merico, Valeria; Prigione, Alessandro; Mulas, Francesca; Sacchi, Lucia; Gabetta, Matteo; Bellazzi, Riccardo; Redi, Carlo Alberto; Mazzini, Giuliano; Adjaye, James; Garagna, Silvia; Zuccotti, Maurizio

doi:10.1186/1471-2164-14-380

Cited by 30 publications

(27 citation statements)

References 28 publications

(39 reference statements)

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“…The integration of biomolecular science, biophysics, and non-invasive analytical methods should vastly improve follicle culture systems, make easier the non-invasive assessment of oocyte nuclear and cytoplasmic development in cultured follicles, and lead to the identification of the molecular signature for oocyte competence. 73 …”

Section: Introductionmentioning

confidence: 99%

Fertility preservation in women with cancer

Vos¹,

Smitz²,

2014

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Enhanced long-term survival rates of young women with cancer and advances in reproductive medicine and cryobiology have culminated in an increased interest in fertility preservation methods in girls and young women with cancer. Present data suggest that young patients with cancer should be referred for fertility preservation counselling quickly to help with their coping process. Although the clinical application of novel developments, including oocyte vitrification and oocyte maturation in vitro, has resulted in reasonable success rates in assisted reproduction programmes, experience with these techniques in the setting of fertility preservation is in its infancy. It is hoped that these and other approaches, some of which are still regarded as experimental (eg, ovarian tissue cryopreservation, pharmacological protection against gonadotoxic agents, in-vitro follicle growth, and follicle transplantation) will be optimised and become established within the next decade. Unravelling the complex mechanisms of activation and suppression of follicle growth will not only expand the care of thousands of women diagnosed with cancer, but also inform the care of millions of women confronted with reduced reproductive fitness because of ageing.

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Section: Introductionmentioning

confidence: 99%

Fertility preservation in women with cancer

Vos¹,

Smitz²,

2014

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“…Previous studies have used microarray analysis and real-time polymerase chain reaction (PCR) to focus on gene expression profiles in granulosa cells (GCs) or CCs in an effort to find potential biomarkers of oocyte quality (Assou et al 2006(Assou et al , 2010Patrizio et al 2007;Huang and Wells 2010;Assidi et al 2011;Vigone et al 2013). Using a non-invasive test to assess embryo potential on the basis of gene expression profiles of human CCs, Assou et al (2008) found that there was a correlation between the expression of some genes in CCs and embryo potential or pregnancy outcome, and proposed BCL2 L11, PCK1 and NFIB as biomarkers for predicting pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes

Chian

et al. 2016

Reprod. Fertil. Dev.

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Cumulus cells (CCs) are distinct from other granulosa cells and the mutual communication between CCs and oocytes is essential for the establishment of oocyte competence. In the present study we assessed genomic expression profiles in mouse CCs before and after oocyte maturation in vitro. Microarray analysis revealed significant changes in gene expression in CCs between the germinal vesicle (GV) and metaphase II (MII) stages, with 2615 upregulated and 2808 downregulated genes. Genes related to epidermal growth factor, extracellular matrix (Ptgs2, Ereg, Tnfaip6 and Efemp1), mitochondrial metabolism (Fdx1 and Aifm2), gap junctions and the cell cycle (Gja1, Gja4, Ccnd2, Ccna2 and Ccnb2) were highlighted as being differentially expressed between the two development stages. Real-time polymerase chain reaction confirmed the validity and reproducibility of the results for the selected differentially expressed genes. Similar expression patterns were identified by western blot analysis for some functional proteins, including EFEMP1, FDX1, GJA1 and CCND2, followed by immunofluorescence localisation. These genes may be potential biomarkers for oocyte developmental competence following fertilisation and will be investigated further in future studies.

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“…To this end, the use of integrative bioinformatics tools has recently provided new insights into the transcriptional and epigenomics programmes that act behind oocyte maturation and the process of folliculogenesis. Whole-genome transcriptional profiles are already available for human, mouse and cow oocytes (Adjaye, 2005;Huang andWells, 2010: Assou et al, 2011;Diedrichs et al, 2012;Labreque and Sirard, 2014) and cumulus cells (Assou et al, 2008;van Montfoort et al, 2008;Ouandaogo et al, 2011;Vigone et al, 2013;Fragouli et al, 2014;Xu et al, 2014). Also, the epigenetic signature (i.e., DNA methylation and histone acetylation and methylation) of the female gamete has been described either for specific genome sequences using the traditional bisulfite conversion and immunofluorescence assays (Endo et al, 2005;Yeo et al, 2005;Hou et al, 2008;Kageyama et al, 2007;Liu et al, 2004;Racedo et al, 2009;Zuccotti et al, 2011;Denomme et al, 2012;Qiao et al, 2010;Pan et al, 2012;De La Fuente et al, 2004;Akiyama et al, 2004;Spinaci et al, 2004) or, for the whole genome, employing the reduced representation bisulfite sequencing (RRBS), a technique that couples the classical bisulfite conversion with the next generation sequencing technology (Smallwood et al, 2011: Tomizawa et al, 2012.…”

Section: Discussionmentioning

confidence: 99%

3D culture of ovarian follicles: a system towards their engineering?

et al. 2015

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Infertility in women is a health priority. Designing a robust culture protocol capable of attaining complete follicle growth is an exciting challenge, for its potential clinical applications, but also as a model to observe and closely study the sequence of molecular events that lie behind the intricate relationship existing between the oocyte and surrounding follicle cells. Here, we describe the procedures used to maintain the ovarian follicle 3D architecture employing a variety of in vitro systems and several types of matrices. Collagen and alginate are the matrices that led to better results, including proof-of-concept of full-term development. Pioneer in its kind, these studies underlie the drawbacks encountered and the need for a culture system that allows more quantitative analyses and predictions, projecting the culture of the ovarian follicle into the realm of tissue engineering.

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Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes

Cited by 30 publications

References 28 publications

Fertility preservation in women with cancer

Fertility preservation in women with cancer

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes

3D culture of ovarian follicles: a system towards their engineering?

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