Transcriptome-based identification and characterization of genes commonly responding to five different insecticides in the diamondback moth, Plutella xylostella

Gao, Yue; Kim, Kyungmun; Kwon, Deok Ho; Jeong, In Hong; Clark, J. Marshall; Lee, Si Hyeock

doi:10.1016/j.pestbp.2017.11.007

Cited by 56 publications

(34 citation statements)

References 30 publications

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“…) as previously observed in human body lice (Yoon et al, ). Recently, significant enhancement of tolerance was also reported in the larvae of diamondback moth, P. xylostella , following brief sublethal treatments of five different insecticides (Gao et al, ). Thus, the induction of tolerance following brief exposures to sublethal doses of insecticides seems to commonly occur across different species of insects.…”

Section: Discussionmentioning

confidence: 86%

RNA interference validation of detoxification genes involved in ivermectin tolerance in Drosophila melanogaster

Kim

Moreau

Ali

et al. 2018

Insect Molecular Biology

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Previously, we observed increased transcription levels of specific cytochrome P450 monooxygenase (P450) and adenosine triphosphate binding cassette (ABC) transporter genes in human body lice, Pediculus humanus humanus, following exposure to ivermectin using the non-invasive induction assay, which resulted in tolerance. To confirm the roles of these genes in induction and tolerance, the robust genetic model insect Drosophila melanogaster was chosen. Orthologous genes corresponding to the body louse P450 (Cyp9f2, Cyp6g2 and Cyp9h1) and ABC transporter (Mrp1, GC1824 as an ABCB type and CG3327 as an ABCG type) genes were selected for in vivo bioassay. Following a brief treatment with a sublethal dose of ivermectin, the mortality response was significantly slower, indicating the presence of tolerance. Concurrently, the transcription levels of Cyp9f2 and Mrp1 at 3 h and those of Cyp6g2, Cyp9h1, Mrp1, CG1824 and CG3327 at 6 h post-treatment were upregulated, indicating gene induction. In behavioural bioassay using GAL4/UAS-RNA interference transgenic fly lines, increased susceptibility to ivermectin was observed following heat shock in the Cyp9f2 , Cyp6g2 , Cyp9h1 , Mrp1 or CG3327-knockdown flies. Considering that these five genes are orthologous to those which had the largest over-expression level following ivermectin-induced tolerance in the body louse, the current results suggest that they are also associated with ivermectin detoxification in D. melanogaster and that body lice and D. melanogaster are likely to share, in part, similar mechanisms of tolerance to ivermectin.

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Section: Discussionmentioning

confidence: 86%

RNA interference validation of detoxification genes involved in ivermectin tolerance in Drosophila melanogaster

Kim

Moreau

Ali

et al. 2018

Insect Molecular Biology

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“…As such, cuticular proteins have been reported to play crucial roles in the insecticide resistance and tolerance of a variety of insect species, including M . persicae , Lymantria dispar , Aphis gossypii and Plutella xylostella 13,16–18 . Indeed, cuticle protein genes (larval cuticle protein A2B/A3A, 111039230/111039229; cuticle protein 111027628, 111034584, 111038352; cuticle protein 12.5/65/38/21/7/3, 111042794/111035272/111032404/111039228, 111039055/111039056/111032178; endocuticle structural glycoprotein SgAbd-2/4/8/9, 111031116/111028315, 111031121/111031114/111041497) were down-regulated in the IR strain of M .…”

Section: Discussionmentioning

confidence: 99%

“…Global surveys of transcriptional changes in insecticide-treated insects could help elucidate the metabolic and regulatory mechanisms that underlie insecticide resistance. Currently, powerful next-generation RNA sequencing (RNA-Seq) technology can be used to determine the gene expression profiles and, thereby, helping to elucidate the development of insecticide resistance 16–18 . In this study, high-throughput RNA-Seq was used to determine the transcriptome profiles of imidacloprid-resistant (IR) and imidacloprid-susceptible (IS) M .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-based identification and characterization of genes responding to imidacloprid in Myzus persicae

Meng

Chen

Zhang

2019

Sci Rep

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Myzus persicae is a serious and widespread agricultural pest, against which, imidacloprid remains an effective control measure. However, recent reports indicate that this aphid has evolved and developed resistance to imidacloprid. This study aimed to elucidate the underlying mechanisms and genetic basis of this resistance by conducting comparative transcriptomics studies on both imidacloprid-resistant (IR) and imidacloprid-susceptible (IS) M. persicae. The comparative analysis identified 252 differentially expressed genes (DEGs) among the IR and IS M. persicae transcriptomes. These candidate genes included 160 and 92 genes that were down- and up-regulated, respectively, in the imidacloprid-resistant strain. Using functional classification in the GO and KEGG databases, 187 DEGs were assigned to 303 functional subcategories and 100 DEGs were classified into 45 pathway groups. Moreover, several genes were associated with known insecticide targets, cuticle, metabolic processes, and oxidative phosphorylation. Quantitative real-time PCR of 10 DEGs confirmed the trends observed in the RNA sequencing expression profiles. These findings provide a valuable basis for further investigation into the complicated mechanisms of imidacloprid resistance in M. persicae.

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“…In this study, 16 P450 genes belonging to the CYP4, CYP6, CYP9 and CYP12 sub-families were overexpressed in fluralaner-treated groups (Additional file 14). Similarly, butene-fipronil, phoxim, cycloxaprid and chlorantraniliprole could significantly enhance the expression of CYP304A, CYP49A1, CYP4C1 and CYP9E2 in Drosophila melanogaster Meigen, B. mori, Periplaneta americana (Linnaeus) and Plutella xylostella Linnaeus, respectively [23][24][25][26]. Also, CYP4V2, CYP6A13-like and CYP12A2 were overexpressed in the insecticide resistant populations of Bemisia tabaci (Gennadius), Aphis glycines Matsumura and M. domestica, respectively [27][28][29].…”

Section: Discussionmentioning

confidence: 96%

Identification of transcriptome and fluralaner responsive genes in the common cutworm Spodoptera litura Fabricius, based on RNA-seq

et al. 2020

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Background: Fluralaner is a novel isoxazoline insecticide with a unique action site on the γ-aminobutyric acid receptor (GABAR), shows excellent activity on agricultural pests including the common cutworm Spodoptera litura, and significantly influences the development and fecundity of S. litura at either lethal or sublethal doses. Herein, Illumina HiSeq Xten (IHX) platform was used to explore the transcriptome of S. litura and to identify genes responding to fluralaner exposure. Results: A total of 16,572 genes, including 451 newly identified genes, were observed in the S. litura transcriptome and annotated according to the COG, GO, KEGG and NR databases. These genes included 156 detoxification enzyme genes [107 cytochrome P450 enzymes (P450s), 30 glutathione S-transferases (GSTs) and 19 carboxylesterases (CarEs)] and 24 insecticide-targeted genes [5 ionotropic GABARs, 1 glutamate-gated chloride channel (GluCl), 2 voltage-gated sodium channels (VGSCs), 13 nicotinic acetylcholine receptors (nAChRs), 2 acetylcholinesterases (AChEs) and 1 ryanodine receptor (RyR)]. There were 3275 and 2491 differentially expressed genes (DEGs) in S. litura treated with LC 30 or LC 50 concentrations of fluralaner, respectively. Among the DEGs, 20 related to detoxification [16 P450s, 1 GST and 3 CarEs] and 5 were growth-related genes (1 chitin and 4 juvenile hormone synthesis genes). For 26 randomly selected DEGs, real-time quantitative PCR (RT-qPCR) results showed that the relative expression levels of genes encoding several P450s, GSTs, heat shock protein (HSP) 68, vacuolar protein sorting-associated protein 13 (VPSAP13), sodium-coupled monocarboxylate transporter 1 (SCMT1), pupal cuticle protein (PCP), protein takeout (PT) and low density lipoprotein receptor adapter protein 1-B (LDLRAP1-B) were significantly up-regulated. Conversely, genes encoding esterase, sulfotransferase 1C4, proton-coupled folate transporter, chitinase 10, gelsolin-related protein of 125 kDa (GRP), fibroin heavy chain (FHC), fatty acid synthase and some P450s were significantly down-regulated in response to fluralaner. Conclusions: The transcriptome in this study provides more effective resources for the further study of S. litura whilst the DEGs identified sheds further light on the molecular response to fluralaner.

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Transcriptome-based identification and characterization of genes commonly responding to five different insecticides in the diamondback moth, Plutella xylostella

Cited by 56 publications

References 30 publications

RNA interference validation of detoxification genes involved in ivermectin tolerance in Drosophila melanogaster

RNA interference validation of detoxification genes involved in ivermectin tolerance in Drosophila melanogaster

Transcriptome-based identification and characterization of genes responding to imidacloprid in Myzus persicae

Identification of transcriptome and fluralaner responsive genes in the common cutworm Spodoptera litura Fabricius, based on RNA-seq

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