Transcriptome-based gene expression profiling of diploid radish (Raphanus sativus L.) and the corresponding autotetraploid

Cheng, Wanwan; Tang, Mingjia; Xie, Yang; Xu, Liang; Wang, Yan; Luo, Xiaobo; Fan, Lu; Liu, Liwang

doi:10.1007/s11033-018-4549-1

Cited by 7 publications

(1 citation statement)

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“…Mainly, in vitro chromosome doubling in crop plants is achieved using haploids (Dhooghe et al 2011). As mitotic polyploidization has shown induction of important morphological and physiological changes in many crops including some Brassica species (Zhang et al 2014, Marfil et al 2018Cheng et al 2019), it is worth studying the effect and efficiency of trifluralin on in vitro cabbage culture morphogenesis and polyploidy induction. To our knowledge, this is the first report on the post effect of trifluralin on cabbage in vitro.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)

Abeyawardana

Viehmannová

Koudela

2021

Journal of Horticultural Research

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Six genotypes of head cabbage (‘Flexima’, ‘Mutsuma’, ‘Septima’, ‘Zeus’, DC6, ‘Ancoma’) have been studied to find an effective method of obtaining polyploids using trifluralin. The effect of various trifluralin concentrations (0.5, 1.0, 1.5, and 2.0 mg·L−1) and treatment conditions (24 °C/24 h, 24 °C/48 h, 30 °C/24 h, and 30 °C/48 h) on organogenesis of organogenic callus, hypocotyl, and seeds was tested. Ploidy level was detected using a flow cytometer. As a result, low survival of hypocotyls and calli was obtained. The shoots induced from calli and hypocotyls failed to develop quality plantlets and most calli and hypocotyls remained recalcitrant for further differentiation. Treated seeds seemed to be promising objects basing on a considerable percentage of plant regeneration. With the increased concentration of trifluralin and increasing press of treatment conditions, the seed germination rate was significantly reduced. Shoot induction from germinated seeds varied among genotypes and treatments. The root induction was independent of treatments but also reduced considerably compared to the control. Under tested conditions, none of the polyploids resulted. Further studies are required to be successful in protocol development.

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