Transcriptome-based analysis reveals that the biosynthesis of anthocyanins is more active than that of flavonols and proanthocyanins in the colorful flowers of Lagerstroemia indica

Yu, Chunmei; Lian, Bolin; Fang, Wei; Guo, Anfang; Ke, Yongchao; Jiang, Yuna; Chen, Yanhong; Liu, Guoyuan; Zhong, Fei; Zhang, Jian

doi:10.1007/s42977-021-00094-0

Cited by 6 publications

(9 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For TFs shown in the correlation network diagram ( Figure 5 ), 11 transcription factors were shown to be related to the synthesis of anthocyanins, among which only one of the three MYBs was negatively correlated with the content of malvidin-3,5-di- O -glucoside. Some studies have reported that the MYB transcription factor in L. indica had a higher preference for the regulation of anthocyanins ( Yu et al, 2021 ). However, how MYB regulated the expression of structural genes and thus influenced the synthesis of anthocyanins is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Some research has been conducted on pigment composition and a preliminary molecular investigation. Four types of anthocyanins are detected in the flowers (delphinidin 3- O -glucoside, petunidin 3- O -glucoside, malvidin 3- O -glucoside, and cyanidin 3- O -glucoside) ( Zhang et al, 2008 ) and seven R2R3-MYB transcription factors (TFs) were identified as probable regulators of flower color ( Yu et al, 2021 ). However, neither the metabolic nor molecular foundation for the L. indica color diversity is entirely clear, and the control of the anthocyanin pathway requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decoding the formation of diverse petal colors of Lagerstroemia indica by integrating the data from transcriptome and metabolome

Hong

Wang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Lagerstroemia indica has great economic value due to its ecological, medicinal, and ornamental properties. Because its bloom color is one of the most essential characteristics, research into its color development is a hot topic. In this study, five representative colored cultivars were chosen, each representing a different color, such as white, red, pink, violet, and purple. Fully bloomed flowers were used to detect flavonoids in the petals. Anthocyanin is the main factor for the color formation of L. indica. 14 anthocyanins were discovered among the 299 flavonoids. Among 14 anthocyanins, malvidin-3,5-di-O-glucoside varied greatly among four colored samples and is the main contributor to color diversity. Transcriptome sequencing revealed that compared to white flowers, Anthocyanin pathway genes appear to be more active in colored samples. Analyzing the correlation network between metabolites and differential expressed genes, 53 key structural genes, and 24 TFs were detected that may play an essential role in the formation of color in L. indica flowers. Among these, the differential expression of F3′5′H and F3′H between all samples are contributors to color diversity. These findings lay the foundation for discovering the molecular mechanism of L. indica flower color diversity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Decoding the formation of diverse petal colors of Lagerstroemia indica by integrating the data from transcriptome and metabolome

Hong

Wang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…PCR mixes were made following the protocols of the TB GREEN kit. The expression levels were calculated using 2 –ΔΔ Ct compared to the internal control and CK sample ( Yu et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…It is also a traditional medicinal plant with several effective secondary metabolites ( Yang et al, 2011 ; Labib et al, 2013 ). During the past decade, research has primarily focused on genes determining ornamental traits, such as leaf and flower color ( Wang et al, 2017 ; Qiao et al, 2019 ; Li et al, 2020a ; Yu et al, 2021 ), as well as the dwarf and weeping architecture ( Ye et al, 2015 ; Li et al, 2020b ). However, during its long life, L. indica faces several challenges, including the threat of diseases and soil stress (nutrient and water shortages, high pH, or high salt in some coastal areas).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of calcineurin B-like protein-interacting protein kinase gene family reveals members participating in abiotic stress in the ornamental woody plant Lagerstroemia indica

Ke²,

Qin³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Calcineurin B-like protein-interacting protein kinases (CIPKs) play important roles in plant responses to stress. However, their function in the ornamental woody plant Lagerstroemia indica is remains unclear. In this study, the LiCIPK gene family was analyzed at the whole genome level. A total of 37 LiCIPKs, distributed across 17 chromosomes, were identified. Conserved motif analysis indicated that all LiCIPKs possess a protein kinase motif (S_TKc) and C-terminal regulatory motif (NAF), while seven LiCIPKs lack a protein phosphatase interaction (PPI) motif. 3D structure analysis further revealed that the N-terminal and C-terminal 3D-structure of 27 members are situated near to each other, while 4 members have a looser structure, and 6 members lack intact structures. The intra- and interspecies collinearity analysis, synonymous substitution rate (Ks) peaks of duplicated LiCIPKs, revealed that ∼80% of LiCIPKs were retained by the two whole genome duplication (WGD) events that occurred approximately 56.12–61.16 million year ago (MYA) and 16.24–26.34 MYA ago. The promoter of each LiCIPK contains a number of auxin, abscisic acid, gibberellic acid, salicylic acid, and drought, anaerobic, defense, stress, and wound responsive cis-elements. Of the 21 members that were successfully amplified by qPCR, 18 LiCIPKs exhibited different expression patterns under NaCl, mannitol, PEG8000, and ABA treatments. Given that LiCIPK30, the AtSOS2 ortholog, responded to all four types of stress it was selected for functional verification. LiCIPK30 complements the atsos2 phenotype in vivo. 35S:LiCIPK-overexpressing lines exhibit increased leaf area increment, chlorophyll a and b content, reactive oxygen species scavenging enzyme activity, and expression of ABF3 and RD22, while the degree of membrane lipid oxidation decreases under NaCl treatment compared to WT. The evolutionary history, and potential mechanism by which LiCIPK30 may regulate plant tolerance to salt stress were also discussed. In summary, we identified LiCIPK members involved in abiotic stress and found that LiCIPK30 transgenic Arabidopsis exhibits more salt and osmotic stress tolerance than WT. This research provides a theoretical foundation for further investigation into the function of LiCIPKs, and for mining gene resources to facilitate the cultivation and breeding of new L. indica varieties in coastal saline-alkali soil.

show abstract

“…In L. indica , some of the genes involved in anthocyanin biosynthesis have been identified based on transcriptomic data [ 2 , 3 , 27 ]. A recent study showed that a bZIP TF LfiHY5 and LfiMYB75 activate the anthocyanin biosynthesis in leaves of a crape myrtle varieties ‘Ebony Embers’ [ 28 ]; However, the mechanism of the biosynthesis of the different types of flavonoids in this plant should be elucidated (Fig.…”

Section: Introductionmentioning

confidence: 99%

Genomic and transcriptomic studies on flavonoid biosynthesis in Lagerstroemia indica

Yu,

Liu,

Qin

et al. 2024

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

Background Lagerstroemia indica is a widely cultivated ornamental woody shrub/tree of the family Lythraceae that is used as a traditional medicinal plant in East Asia and Egypt. However, unlike other ornamental woody plants, its genome is not well-investigated, which hindered the discovery of the key genes that regulate important traits and the synthesis of bioactive compounds. Results In this study, the genomic sequences of L. indica were determined using several next-generation sequencing technologies. Altogether, 324.01 Mb sequences were assembled and 98.21% (318.21 Mb) of them were placed in 24 pseudo-chromosomes. The heterozygosity, repeated sequences, and GC residues occupied 1.65%, 29.17%, and 38.64% of the genome, respectively. In addition, 28,811 protein-coding gene models, 327 miRNAs, 552 tRNAs, 214 rRNAs, and 607 snRNAs were identified. The intra- and interspecies synteny and Ks analysis revealed that L. indica exhibits a hexaploidy. The co-expression profiles of the genes involved in the phenylpropanoid (PA) and flavonoid/anthocyanin (ABGs) pathways with the R2R3 MYB genes (137 members) showed that ten R2R3 MYB genes positively regulate flavonoid/anthocyanin biosynthesis. The colors of flowers with white, purple (PB), and deep purplish pink (DPB) petals were found to be determined by the levels of delphinidin-based (Dp) derivatives. However, the substrate specificities of LiDFR and LiOMT probably resulted in the different compositions of flavonoid/anthocyanin. In L. indica, two LiTTG1s (LiTTG1-1 and LiTTG1-2) were found to be the homologs of AtTTG1 (WD40). LiTTG1-1 was found to repress anthocyanin biosynthesis using the tobacco transient transfection assay. Conclusions This study showed that the ancestor L. indica experienced genome triplication approximately 38.5 million years ago and that LiTTG1-1 represses anthocyanin biosynthesis. Furthermore, several genes such as LiDFR, LiOMTs, and R2R3 LiMYBs are related to anthocyanin biosynthesis. Further studies are required to clarify the mechanisms and alleles responsible for flower color development.

show abstract

Transcriptome-based analysis reveals that the biosynthesis of anthocyanins is more active than that of flavonols and proanthocyanins in the colorful flowers of Lagerstroemia indica

Cited by 6 publications

References 51 publications

Decoding the formation of diverse petal colors of Lagerstroemia indica by integrating the data from transcriptome and metabolome

Decoding the formation of diverse petal colors of Lagerstroemia indica by integrating the data from transcriptome and metabolome

Genome-wide identification of calcineurin B-like protein-interacting protein kinase gene family reveals members participating in abiotic stress in the ornamental woody plant Lagerstroemia indica

Genomic and transcriptomic studies on flavonoid biosynthesis in Lagerstroemia indica

Contact Info

Product

Resources

About