Transcriptome and Resequencing Analyses Provide Insight into Differences in Organic Acid Accumulation in Two Pear Varieties

Li, Qionghou; Qiao, Xin; Jia, Luting; Zhang, Yuxin; Zhang, Shaoling

doi:10.3390/ijms22179622

Cited by 6 publications

(2 citation statements)

References 70 publications

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“…Here we also found that the malate contents (2.5 mg/g) are higher than the citrate contents (1.5 mg/g) in the pulp of a ripe pear fruit (Figure 1C). In plants, malate regulation was also widely studied among different species of pear fruit [10,39,40]. For example, cMDH and MS are considered important enzymes to produce malic acid, while mMDH and ME are the key enzymes to degrade malate into oxaloacetate and pyruvate, respectively [11].…”

Section: Discussionmentioning

confidence: 99%

Diversity in Acidity between Core and Pulp of Asian Pear Fruit Is Mainly Regulated by the Collaborative Activity of PH8.1 and DIC2 Genes during Fruit Development

et al. 2022

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The pear (Pyrus pyrifolia) is an important accessory fruit in which the pear core is tarter than the pear pulp. However, the reason for the acidic core and diversity in the taste of the same fruit is not clear. In this study, we observed that the citrate contents were three times higher in the core than in the pulp, while the malate content decreased along with fruit development and was significantly lower in the core than in the pulp at 110 days after flowering. Overall transcript levels for citrate-malate synthesis-related genes increased more in the pear core than the pulp at early fruit development, while degradation-related genes activity was nearly similar or non-significant between the core and pulp during fruit development. The lesser malate accumulation in the pear core compared to the pulp at 110 DAF was possibly due to the reduced activity of tDT2 gene. Regarding citrate accumulation, we identified five important p-type H+-ATPase genes in pear and found that the relative expression level of the PH8.1 gene was four-fold higher in the core than in the pulp during fruit development. Moreover, the expression level of di-carboxylate carrier gene 2 (DIC2) was constantly and significantly higher in the core than in the pulp. In addition, correlation analysis signified that the transcript levels of the two genes PH8.1 and DIC2 positively and significantly correlated with the citrate contents. These results suggested that the increased and collaborative activity of PH8.1 and DIC2 played a key role in the higher citrate accumulation in the core than the pulp, thus, with the help of molecular breeding tools, the citrate contents can be optimized in pear fruit for divers and improved fruit flavoring.

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Section: Discussionmentioning

confidence: 99%

Diversity in Acidity between Core and Pulp of Asian Pear Fruit Is Mainly Regulated by the Collaborative Activity of PH8.1 and DIC2 Genes during Fruit Development

et al. 2022

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“…RNA-seq was conducted for ‘Dangshansuli’ pulp collected from different developmental stages (15, 45, 90, 120 and 145 DAF) with three replicates [ 87 ]. Adapter, ploy-N and low-quality reads were removed from the raw reads to produce clean reads.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification, comparative analysis and functional roles in flavonoid biosynthesis of cytochrome P450 superfamily in pear (Pyrus spp.)

Zhang,

Li,

et al. 2023

BMC Genom Data

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Background The cytochrome P450 (CYP) superfamily is the largest enzyme metabolism family in plants identified to date, and it is involved in many biological processes, including secondary metabolite biosynthesis, hormone metabolism and stress resistance. However, the P450 gene superfamily has not been well studied in pear (Pyrus spp.). Results Here, the comprehensive identification and a comparative analysis of P450 superfamily members were conducted in cultivated and wild pear genomes. In total, 338, 299 and 419 P450 genes were identified in Chinese white pear, European pear and the wild pear, respectively. Based on the phylogenetic analyses, pear P450 genes were divided into ten clans, comprising 48 families. The motif and gene structure analyses further supported this classification. The expansion of the pear P450 gene family was attributed to whole-genome and single-gene duplication events. Several P450 gene clusters were detected, which have resulted from tandem and proximal duplications. Purifying selection was the major force imposed on the long-term evolution of P450 genes. Gene dosage balance, subfunctionalization and neofunctionalization jointly drove the retention and functional diversification of P450 gene pairs. Based on the association analysis between transcriptome expression profiles and flavonoid content during fruit development, three candidate genes were identified as being closely associated with the flavonoid biosynthesis, and the expression of one gene was further verified using qRT-PCR and its function was validated through transient transformation in pear fruit. Conclusions The study results provide insights into the evolution and biological functions of P450 genes in pear.

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Specific physiological responses to alkaline carbonate stress in rice (Oryza sativa) seedlings: organic acid metabolism and hormone signalling

Wang,

Xu,

et al. 2024

Funct. Plant Biol.

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In recent years, alkaline soda soil has stimulated numerous biological research on plants under carbonate stress. Here, we explored the difference in physiological regulation of rice seedlings between saline (NaCl) and alkaline carbonate (NaHCO3 and Na2CO3) stress. The rice seedlings were treated with 40 mM NaCl, 40 mM NaHCO3 and 20 mM Na2CO3 for 2 h, 12 h, 24 h and 36 h, their physiological characteristics were determined, and organic acid biosynthesis and metabolism and hormone signalling were identified by transcriptome analysis. The results showed that alkaline stress caused greater damage to their photosynthetic and antioxidant systems and led to greater accumulation of organic acid, membrane damage, proline and soluble sugar but a decreased jasmonic acid content compared with NaCl stress. Jasmonate ZIM-Domain (JAZ), the probable indole-3-acetic acid-amido synthetase GH3s, and the protein phosphatase type 2Cs that related to the hormone signalling pathway especially changed under Na2CO3 stress. Further, the organic acid biosynthesis and metabolism process in rice seedlings were modified by both Na2CO3 and NaHCO3 stresses through the glycolate/glyoxylate and pyruvate metabolism pathways. Collectively, this study provides valuable evidence on carbonate-responsive genes and insights into the different molecular mechanisms of saline and alkaline stresses.

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Transcriptome and Resequencing Analyses Provide Insight into Differences in Organic Acid Accumulation in Two Pear Varieties

Cited by 6 publications

References 70 publications

Diversity in Acidity between Core and Pulp of Asian Pear Fruit Is Mainly Regulated by the Collaborative Activity of PH8.1 and DIC2 Genes during Fruit Development

Diversity in Acidity between Core and Pulp of Asian Pear Fruit Is Mainly Regulated by the Collaborative Activity of PH8.1 and DIC2 Genes during Fruit Development

Genome-wide identification, comparative analysis and functional roles in flavonoid biosynthesis of cytochrome P450 superfamily in pear (Pyrus spp.)

Specific physiological responses to alkaline carbonate stress in rice (Oryza sativa) seedlings: organic acid metabolism and hormone signalling

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