“…Additionally, it is bound by transcription factors that establish endometrial stromal cell-type identity and mediate decidualization, including the progesterone receptor (PR), NR2F2 (COUP-TFII), GATA2, FOSL2, FOXO1, as well as polymerase II ( Figure 2C ). The HAND2 promoter loops to several distal enhancers, as assessed by H3K27ac HiChIP data generated from a normal hTERT-immortalized endometrial cell line (E6E7hTERT), including a region bound by PR, NR2F2, GATA2, FOSL2 and FOXO1, that also contains SNPs associated with gestation length in recent GWAS (Warrington et al ., 2019; Sakabe et al ., 2020) ( Figure 2C ). HAND2 was significantly up-regulated by decidualization of human ESFs into DSCs by cAMP/progesterone treatment (Log 2 FC=1.28, P=2.62×10 −26 , FDR=1.16×10 −24 ), and significantly down-regulated by siRNAs targeting PR (Log 2 FC=−0.90, P=7.05×10 −15 , FDR=2.03×10 −13 ) and GATA2 (Log 2 FC=−2.73, P=0.01, FDR=0.19) ( Figure 2D ).…”