Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae

Shelomi, Matan; Lin, Shih-Shun; Liu, Liyu

doi:10.1186/s12864-019-6352-3

Cited by 32 publications

(36 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A broader study should be performed to discriminate among some potential tissues where components of the insect humoral immune system are known to be expressed [46][47][48][49] , such as fat body, haemocytes, gut, salivary glands, trachea, Malpighian tubules, and integument. Fat bodies have been reported to display high expression level for some AMP types such as defensins 49,50 and attacin 51 . Defensin genes from adult A. stephensi are expressed in fat body, midgut, haemolymph and salivary glands with the high level of expression at fat body 49 .…”

Section: Discussionmentioning

confidence: 99%

Blattella germanica displays a large arsenal of antimicrobial peptide genes

Silva

Muñoz-Benavent

García-Ferris

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Defence systems against microbial pathogens are present in most living beings. The German cockroach Blattella germanica requires these systems to adapt to unhealthy environments with abundance of pathogenic microbes, in addition to potentially control its symbiotic systems. To handle this situation, four antimicrobial gene families (defensins, termicins, drosomycins and attacins) were expanded in its genome. Remarkably, a new gene family (blattellicins) emerged recently after duplication and fast evolution of an attacin gene, which is now encoding larger proteins with the presence of a long stretch of glutamines and glutamic acids. Phylogenetic reconstruction, within Blattellinae, suggests that this duplication took place before the divergence of Blattella and Episymploce genera. The latter harbours a long attacin gene (pre-blattellicin), but the absence of the encoded Glx-region suggests that this element evolved recently in the Blattella lineage. A screening of AMP gene expression in available transcriptomic SR projects of B. germanica showed that, while some AMPs are expressed during almost the whole development, others are restricted to shorter periods. Blattellicins are highly expressed only in adult females. None of the available SR tissue projects could be associated with blattellicins’ expression, suggesting that it takes place in other tissues, maybe the gut.

show abstract

Section: Discussionmentioning

confidence: 99%

Blattella germanica displays a large arsenal of antimicrobial peptide genes

Silva

Muñoz-Benavent

García-Ferris

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The complete mitogenome sequence was initially annotated using the MITOS web server (Bernt et al 2013), and tRNA genes and their secondary structures were cross-analysed using tRNAscan-SE v2.0 (Chan et al, 2019). To further refine the annotation and to examine mitogenome transcription, we used BWA-MEM to align Illumina reads from a transcriptome study of O. rhinoceros larvae (Shelomi, Lin, and Liu 2019), retrieved from the NCBI (SRR9208133). Finally, we manually inspected and compared our annotation to the complete and near complete mitogenome annotations of other related taxa (Table 1) in Geneious (2020.0.4).…”

Section: Genome Assembly Annotation and Analysismentioning

confidence: 99%

The complete mitochondrial genome sequence ofOryctes rhinoceros(Coleoptera: Scarabaeidae) based on long-read nanopore sequencing

Filipović

Hereward

Rašić

et al. 2020

Preprint

View full text Add to dashboard Cite

The coconut rhinoceros beetle (CRB, Oryctes rhinoceros) is a severe and invasive pest of coconut and other palms throughout Asia and the Pacific. The biocontrol agent, Oryctes rhinoceros nudivirus (OrNV), has successfully suppressed O. rhinoceros populations for decades but new CRB invasions started appearing after 2007. A single-SNP variant within the mitochondrial cox1 gene is used to distinguish the recently-invading CRB-G lineage from other haplotypes, but the lack of mitogenome sequence for this species hinders further development of a molecular toolset for biosecurity and management programmes against CRB. Here we report the complete circular sequence and annotation for CRB mitogenome, generated to support such efforts.Sequencing data were generated using long-read Nanopore technology from genomic DNA isolated from a CRB-G female. The mitochondrial genome was assembled with Flye v.2.5, using the short-read Illumina sequences to remove homopolymers with Pilon, and annotated with MITOS. Independently-generated transcriptome data were used to assess the O. rhinoceros mitogenome annotation and transcription. The aligned sequences of 13 protein-coding genes (PCGs) (with degenerate third codon position) from O. rhinoceros, 13 other Scarabaeidae taxa and two outgroup taxa were used for the phylogenetic reconstruction with the Maximum likelihood (ML) approach in IQ-TREE and Bayesian (BI) approach in MrBayes.The complete circular mitochondrial genome of O. rhinoceros is 20,898 bp-long, with a gene content canonical for insects (13 PCGs, 2 rRNA genes, and 22 tRNA genes), as well as one structural variation (rearrangement of trnQ and trnI) and a long control region (6,204 bp). Transcription was detected across all 37 genes, and interestingly, within three domains in the control region. ML and BI phylogenies had the same topology, correctly grouping O. rhinoceros with one other Dynastinae taxon, and recovering the previously reported relationship among lineages in the Scarabaeidae. In silico PCR-RFLP analysis recovered the correct fragment set that is diagnostic for the CRB-G haplogroup. These results validate the high-quality of the CRB mitogenome sequence and annotation.

show abstract

“…A study on important role of Collembola as a decomposer of soil organic matter by Rahardjo, Rachmawati, & Soetjipto (2019) inspiring to study the role of rhinoceros beetle larvae as a decomposer in the oil palm plantations. Shelomi, Lin, & Liu, (2019) reported that the gut microbiota of O. rhinoceros larvae is quite similar to that of the termite gut, as both species feed on decaying wood. Moreover, the beetle larvae are suggested to be used as feed and food (Okaraonye & Ikewuchi, 2009;Omotoso, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Potency of Collecting Larvae of Oryctes rhinoceros L. (Coleoptera:Scarabaeidae) in the Oil Palm Plantation

2020

View full text Add to dashboard Cite

The intensive and continuous collection of rhinoceros beetles, Oryctes rhinoceros L. (Coleoptera: Scarabaeidae), is an extraordinary strategy in IPM (Integrated Pest Management). This study was intended to evaluate the potency of people in collecting larvae and to determine the age structure of the beetle population. The study was conducted in Asahan Regency, North Sumatera Province. Adult men, adult women, adolescent girls, and adolescent boys collected beetles for 2 hours in each breeding site. Results showed that the beetle was more significant abundant in decomposed trunks at plots of immature and mature palms than at heaps of the empty fruit bunches namely 236, 221 and 93 individuals/2 hours. The population age-structure showed significantly predominant of 2 nd and 3 rd instars in all breeding sites, i.e. eggs, 1 st instars, 2 nd instars, and 3 rd instars, pupae, adult males, and adult females were 2.02, 23.29, 21.39, 47.06, 3.17, 0.86, and 2.21%, respectively. Adult men, adult women, and adolescent boys had similar potency in collecting 3 rd instar larvae while the least amount was collected by the adolescent girls viz. 70, 85, 60, and 38 larvae/2 hours, respectively. It is suggested that the study results support future efforts in the sustainable control of rhinoceros beetle through collecting larvae for commercial utilization.

show abstract

Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae

Cited by 32 publications

References 86 publications

Blattella germanica displays a large arsenal of antimicrobial peptide genes

Blattella germanica displays a large arsenal of antimicrobial peptide genes

The complete mitochondrial genome sequence ofOryctes rhinoceros(Coleoptera: Scarabaeidae) based on long-read nanopore sequencing

The Potency of Collecting Larvae of Oryctes rhinoceros L. (Coleoptera:Scarabaeidae) in the Oil Palm Plantation

Contact Info

Product

Resources

About