Transcriptome and Metabolome Profiles Revealed Molecular Mechanisms Underlying Tolerance of Portulaca oleracea to Saline Stress

Xing, Jin-Cheng; Zhao, Baoquan; Dong, Jianzhang; Liu, C.; Wen, Zhugui; Zhu, Xiaoming; Ding, Haiping; He, Tengbing; Yang, Haijun; Wang, M. W.; Hong, Lei

doi:10.1134/s1021443720010240

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…Since, the development of high-throughput sequencing technology, transcripts sequencing has become an important means to study gene expression regulation, after the whole-genome sequencing of human were completed in 2004 [ 13 ]. However, due to the read length limitation of the second-generation sequencing in different organisms [ 14 , 15 , 16 , 17 , 18 ], the full-length transcript obtained by splicing is not complete. The third-generation sequencing technology signified by Pacific Biosciences (PacBio, Menlo Park, CA, USA) effectively overcome this problem [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Long-Read Sequencing of Purslane (Portulaca oleracea) and Differential Gene Expression Related with Biosynthesis of Unsaturated Fatty Acids

Zaman

et al. 2021

Plants

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This study aimed to obtain the full-length transcriptome of purslane (Portulaca oleracea); assorted plant samples were used for single-molecule real-time (SMRT) sequencing. Based on SMRT, functional annotation of transcripts, transcript factors (TFs) analysis, simple sequence repeat analysis and long non-coding RNAs (LncRNAs) prediction were accomplished. Total 15.33-GB reads were produced; with 9,350,222 subreads and the average length of subreads, 1640 bp was counted. With 99.99% accuracy, after clustering, 132,536 transcripts and 78,559 genes were detected. All unique SMART transcripts were annotated in seven functional databases. 4180 TFs (including transcript regulators) and 7289 LncRNAs were predicted. The results of RNA-seq were confirmed with qRT–PCR analysis. Illumina sequencing of leaves and roots of two purslane genotypes was carried out. Amounts of differential expression genes and related KEGG pathways were found. The expression profiles of related genes in the biosynthesis of unsaturated fatty acids pathway in leaves and roots of two genotypes of purslane were analyzed. Differential expression of genes in this pathway built the foundation of ω-3 fatty acid accumulation in different organs and genotypes of purslane. The aforementioned results provide sequence information and may be a valuable resource for whole-genome sequencing of purslane in the future.

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Section: Introductionmentioning

confidence: 99%

Single-Molecule Long-Read Sequencing of Purslane (Portulaca oleracea) and Differential Gene Expression Related with Biosynthesis of Unsaturated Fatty Acids

Zaman

et al. 2021

Plants

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“…Besides having an extraordinary nutrient profile, Portulaca shows excellent tolerance towards salinity stress and drought stress. The transcriptome sequencing and metabolome analysis of P. oleracea regarding salinity tolerance were conducted by Xing et al [ 417 ]. They reported that genes of photosynthesis and aquaporins were depressed during salinity treatment which indicates the inhibition of photosynthesis and water uptake during salinity stress.…”

Section: Examples Of Application Of Multi-omics Tools To Traditional Food Plantsmentioning

confidence: 99%

Integrating Omics and Gene Editing Tools for Rapid Improvement of Traditional Food Plants for Diversified and Sustainable Food Security

Kumar

Anju

Kumar³

et al. 2021

IJMS

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Indigenous communities across the globe, especially in rural areas, consume locally available plants known as Traditional Food Plants (TFPs) for their nutritional and health-related needs. Recent research shows that many TFPs are highly nutritious as they contain health beneficial metabolites, vitamins, mineral elements and other nutrients. Excessive reliance on the mainstream staple crops has its own disadvantages. Traditional food plants are nowadays considered important crops of the future and can act as supplementary foods for the burgeoning global population. They can also act as emergency foods in situations such as COVID-19 and in times of other pandemics. The current situation necessitates locally available alternative nutritious TFPs for sustainable food production. To increase the cultivation or improve the traits in TFPs, it is essential to understand the molecular basis of the genes that regulate some important traits such as nutritional components and resilience to biotic and abiotic stresses. The integrated use of modern omics and gene editing technologies provide great opportunities to better understand the genetic and molecular basis of superior nutrient content, climate-resilient traits and adaptation to local agroclimatic zones. Recently, realizing the importance and benefits of TFPs, scientists have shown interest in the prospection and sequencing of TFPs for their improvements, cultivation and mainstreaming. Integrated omics such as genomics, transcriptomics, proteomics, metabolomics and ionomics are successfully used in plants and have provided a comprehensive understanding of gene-protein-metabolite networks. Combined use of omics and editing tools has led to successful editing of beneficial traits in several TFPs. This suggests that there is ample scope for improvement of TFPs for sustainable food production. In this article, we highlight the importance, scope and progress towards improvement of TFPs for valuable traits by integrated use of omics and gene editing techniques.

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“…Since purslane is known for its medicinal and nutritional properties due to the synthesis of important nutrients and phytochemicals such as alkaloids, flavonoids, catecholamines, lignans, terpenoids, betalains, carotenoids, vitamins, and ω-3-fatty acids (Oliveira et al, 2009;Mulla and Swamy, 2010;Singh et al, 2011;Patil et al, 2012;Petropoulos et al, 2016;Verma et al, 2016;Fernández-Poyatos et al, 2021) and tolerance toward several stresses such as drought, temperature, salinity, moisture, and heat (Ichimura and Suto, 1998;Yazici et al, 2007;Alam et al, 2014b;Jin et al, 2016;Borsai et al, 2020;Xing et al, 2020), it is attaining greater attention (Sultana and Raheman, 2013;Uddin et al, 2014). Many plant-derived secondary metabolites have been used as drugs in modern times as these compounds have specific biological activities (Kumar et al, 2019).…”

Section: Genetics Of Phytochemical Nutritional and Stress-resilient Traitsmentioning

confidence: 99%

Improvement of a Traditional Orphan Food Crop, Portulaca oleracea L. (Purslane) Using Genomics for Sustainable Food Security and Climate-Resilient Agriculture

Kumar

Sreedharan

Singh

et al. 2021

Front. Sustain. Food Syst.

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Purslane (Portulaca oleracea L.) is a popular orphan crop used for its nutritional properties in various parts of the world. It is considered one of the richest terrestrial sources of omega-3 and omega-6-fatty acids (ω-3 and 6-FAs) suggesting its importance for human health. This ethnomedicinal plant is also an important part of traditional healing systems among the indigenous people. Many studies have indicated its tolerance against multiple stresses and found that it easily grows in a range of environmental gradients. It has also been considered one of the important biosaline crops for the future. Despite its huge nutritional, economic, and medicinal importance, it remains neglected to date. Most of the studies on purslane were focused on its ethnomedicinal, phytochemical, pharmacological, and stress-tolerance properties. Only a few studies have attempted genetic dissection of the traits governing these traits. Purslane being an important traditional food crop across the globe can be valorized for a sustainable food security in the future. Therefore, this review is an attempt to highlight the distribution, domestication, and cultivation of purslane and its importance as an important stress-tolerant food and a biosaline crop. Furthermore, identification of genes and their functions governing important traits and its potential for improvement using genomics tools for smart and biosaline agriculture has been discussed.

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Transcriptome and Metabolome Profiles Revealed Molecular Mechanisms Underlying Tolerance of Portulaca oleracea to Saline Stress

Cited by 10 publications

References 26 publications

Single-Molecule Long-Read Sequencing of Purslane (Portulaca oleracea) and Differential Gene Expression Related with Biosynthesis of Unsaturated Fatty Acids

Single-Molecule Long-Read Sequencing of Purslane (Portulaca oleracea) and Differential Gene Expression Related with Biosynthesis of Unsaturated Fatty Acids

Integrating Omics and Gene Editing Tools for Rapid Improvement of Traditional Food Plants for Diversified and Sustainable Food Security

Improvement of a Traditional Orphan Food Crop, Portulaca oleracea L. (Purslane) Using Genomics for Sustainable Food Security and Climate-Resilient Agriculture

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