Transcriptome and metabolome analyses reveal phenotype formation differences between russet and non-russet apples

Wang, Ziqi; Li, Shasha; Huo, Wenping; Chen, Min; Zhang, Yugang; Jiang, Shenghui

doi:10.3389/fpls.2022.1057226

Cited by 3 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is the first 40 days after full bloom [ 1 , 12 , 20 , 33 – 36 ]. However, most studies of transcriptomes of russeted apple fruit are based on natural russeting assessed at the mature stage [ 24 – 26 , 29 – 32 ]. Furthermore, the beginning of russet induction is precisely defined in moisture-induced russeting.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, investigations at the multispecies level revealed MYB9 and MYB107 to be major regulators of suberin formation in angiosperms [ 29 ]. The majority of transcription factors associated with the later processes of russeting belong to the R2R3-MYB family and, to a minor extent, to the AP2/EREBP, bHLH, C2H2, WRKY, and NAC-domain transcription factor families [ 25 – 27 , 30 – 32 ]. Unfortunately, all of the above analyses were conducted on fruit at the mature stage, while russeting typically occurs during early development.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Time course of changes in the transcriptome during russet induction in apple fruit

Straube,

Suvarna,

Chen

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background Russeting is a major problem in many fruit crops. Russeting is caused by environmental factors such as wounding or moisture exposure of the fruit surface. Despite extensive research, the molecular sequence that triggers russet initiation remains unclear. Here, we present high-resolution transcriptomic data by controlled russet induction at very early stages of fruit development. During Phase I, a patch of the fruit surface is exposed to surface moisture. For Phase II, moisture exposure is terminated, and the formerly exposed surface remains dry. We targeted differentially expressed transcripts as soon as 24 h after russet induction. Results During moisture exposure (Phase I) of ‘Pinova’ apple, transcripts associated with the cell cycle, cell wall, and cuticle synthesis (SHN3) decrease, while those related to abiotic stress increase. NAC35 and MYB17 were the earliest induced genes during Phase I. They are therefore linked to the initial processes of cuticle microcracking. After moisture removal (Phase II), the expression of genes related to meristematic activity increased (WOX4 within 24 h, MYB84 within 48 h). Genes related to lignin synthesis (MYB52) and suberin synthesis (MYB93, WRKY56) were upregulated within 3 d after moisture removal. WOX4 and AP2B3 are the earliest differentially expressed genes induced in Phase II. They are therefore linked to early events in periderm formation. The expression profiles were consistent between two different seasons and mirrored differences in russet susceptibility in a comparison of cultivars. Furthermore, expression profiles during Phase II of moisture induction were largely identical to those following wounding. Conclusions The combination of a unique controlled russet induction technique with high-resolution transcriptomic data allowed for the very first time to analyse the formation of cuticular microcracks and periderm in apple fruit immediately after the onset of triggering factors. This data provides valuable insights into the spatial-temporal dynamics of russeting, including the synthesis of cuticles, dedifferentiation of cells, and impregnation of cell walls with suberin and lignin.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Time course of changes in the transcriptome during russet induction in apple fruit

Straube,

Suvarna,

Chen

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…Although some studies have been conducted on gene expression and phenotype variations in apples 6 – 8 , 11 – 16 . there are several limitations in terms of data quality and completeness.…”

Section: Background and Summarymentioning

confidence: 99%

Phenomics and transcriptomic profiling of fruit development in distinct apple varieties

Zhang,

Han,

Liao

2024

Sci Data

View full text Add to dashboard Cite

Apple is one of the most economically important and popular temperate fruit trees. The domestication of apple has resulted in substantial phenotypic differences, particularly between wild and cultivated varieties. However, the relationship between gene expression and phenotypic variations in apple remains poorly understood. Here, we present a comprehensive dataset featuring five distinct apple varieties, including two wild varieties and three representative cultivated varieties. The dataset comprises of both phenomics data, encompassing twelve fruit quality-related traits continuously measured over two years, and transcriptomic data obtained at different developmental stages with three biological replicates. We performed basic quality control process, gene expression normalization and differential gene expression analysis to demonstrate the utility and reliability of the dataset. Our findings indicate that gene expression strongly related with phenotypic variations in apple. This dataset serves as a valuable resource, encompassing phenomics and transcriptomic data in multiple formats, thereby facilitating further exploration of the relationships between gene expression and phenotypic traits in apple.

show abstract

Influence of the content of biologically active phenolic compounds in the fruits of Malus Mill. on their quality indicators

Feshchenko

2023

Plodovodstvo i âgodovodstvo Rossii

View full text Add to dashboard Cite

The article presents the results of an analytical review of Russian and foreign literature sources devoted to the study of the influence of the composition and functions of phenolic compounds in apple fruits on their quality indicators (appearance, pulp density, nutritional value). Studies of the biochemical composition of apple fruits are currently relevant and are being carried out with the aim of in-depth study of the assortment of fruit crops and assessment of its potential use in breeding to increase the content of biologically active compounds. The prospects of the search for plants that accumulate phenolic compounds in tissues, which have a protective antioxidant function and nutritional value, have been confirmed. The issue of the role of accumulation, the dynamics of the content of individual phenolic compounds and the total content of phenolic compounds in various metabolic processes that determine the properties of apple fruits is considered. Analysis of the results of thematic scientific works on changes in the composition and content of phenolic compounds will allow targeted research leading to the use of fruits as a potential source of phenolic compounds, as well as identifying sources of accumulation of biologically active substances for practical use in breeding. The creation of new varieties of apple trees is necessary to obtain useful secondary phenolic metabolites contained in fruits, while the range of use of fruits includes the production of processed products. The expediency of carrying out breeding work aimed at increasing the content of phenolic compounds in fruits is confirmed by a number of recent publications by Russian and foreign scientists.

show abstract

Transcriptome and metabolome analyses reveal phenotype formation differences between russet and non-russet apples

Cited by 3 publications

References 39 publications

Time course of changes in the transcriptome during russet induction in apple fruit

Time course of changes in the transcriptome during russet induction in apple fruit

Phenomics and transcriptomic profiling of fruit development in distinct apple varieties

Influence of the content of biologically active phenolic compounds in the fruits of Malus Mill. on their quality indicators

Contact Info

Product

Resources

About